FNIP1 Rabbit mAb

Catalog No.: F3977

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:50 - 1:100

Application
WB, IHC

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
131 kDa

Datasheet & SDS

Biological Description

Specificity
FNIP1 Rabbit mAb detects endogenous levels of total FNIP1 protein.

Clone
B16M7

Synonym(s)
KIAA1961, FNIP1, Folliculin-interacting protein 1

Background
FNIP1 (Folliculin-interacting protein 1) is a highly conserved cytoplasmic adaptor protein that regulates cellular metabolism, immune homeostasis, and organelle function by integrating key signaling pathways such as mTORC1 and AMPK. Structurally, FNIP1 contains longin, degron, and DENN-like domains, along with multiple phosphorylation, glycosylation, and ubiquitination sites that dynamically regulate its stability and function. FNIP1 is widely expressed, with particularly high levels in immune-related tissues (thymus, spleen, bone marrow) and metabolically active organs (liver, kidney, heart). Functionally, it interacts with folliculin (FLCN), AMPK, and HSP90 to coordinate nutrient sensing, mitochondrial and lysosomal biogenesis, proteostasis, and immune cell development. FNIP1 deficiency, an autosomal recessive inborn error of immunity, leads to profound B-cell defects, immunodysregulation, metabolic abnormalities, and multisystem involvement, highlighting its critical role in linking metabolic signaling with immune and tissue homeostasis.

Background

FNIP1 (Folliculin-interacting protein 1) is a highly conserved cytoplasmic adaptor protein that regulates cellular metabolism, immune homeostasis, and organelle function by integrating key signaling pathways such as mTORC1 and AMPK. Structurally, FNIP1 contains longin, degron, and DENN-like domains, along with multiple phosphorylation, glycosylation, and ubiquitination sites that dynamically regulate its stability and function. FNIP1 is widely expressed, with particularly high levels in immune-related tissues (thymus, spleen, bone marrow) and metabolically active organs (liver, kidney, heart). Functionally, it interacts with folliculin (FLCN), AMPK, and HSP90 to coordinate nutrient sensing, mitochondrial and lysosomal biogenesis, proteostasis, and immune cell development. FNIP1 deficiency, an autosomal recessive inborn error of immunity, leads to profound B-cell defects, immunodysregulation, metabolic abnormalities, and multisystem involvement, highlighting its critical role in linking metabolic signaling with immune and tissue homeostasis.

References
https://pubmed.ncbi.nlm.nih.gov/40699689/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%