Fibronectin/FN1 Rabbit mAb

Catalog No.: F0886

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:100 IHC1:100 - 1:400 IF1:200 - 1:800

Application
WB, IP, IHC, IF

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
300 kDa

Datasheet & SDS

Biological Description

Specificity
Fibronectin/FN1 Rabbit mAb recognizes endogenous levels of total Fibronectin/FN1 protein.

Clone
C12E22

Synonym(s)
Fibronectin, Cold-insoluble globulin (CIG), FN1, FN

Background
Fibronectin (Fn) is a multidomain glycoprotein widely distributed in extracellular fluids and matrices across virtually all vertebrate tissues and organs. It serves as a crucial mediator connecting cells to the extracellular matrix (ECM) and plays essential roles in numerous physiological processes, including cell adhesion, differentiation, growth, and migration. Fn also contributes significantly to embryonic development, wound healing, and blood coagulation. Disruptions in fibronectin expression, degradation, or structural organization are associated with various pathological conditions, such as cancer progression and fibrotic diseases. Despite being encoded by a single gene, alternative splicing of its pre-mRNA generates at least 20 different isoforms in humans. Fibronectin exists in two main forms: a soluble form found in body fluids and an insoluble form incorporated into extracellular matrices and basement membranes. In its soluble state, fibronectin adopts a compact structure due to intramolecular interactions. However, when incorporated into the ECM through a process known as fibronectin assembly, the protein unfolds into an extended conformation. Soluble fibronectin is primarily synthesized by hepatocytes and released into the bloodstream, while fibroblasts and endothelial cells are responsible for producing the insoluble, matrix-associated form.

Background

Fibronectin (Fn) is a multidomain glycoprotein widely distributed in extracellular fluids and matrices across virtually all vertebrate tissues and organs. It serves as a crucial mediator connecting cells to the extracellular matrix (ECM) and plays essential roles in numerous physiological processes, including cell adhesion, differentiation, growth, and migration. Fn also contributes significantly to embryonic development, wound healing, and blood coagulation. Disruptions in fibronectin expression, degradation, or structural organization are associated with various pathological conditions, such as cancer progression and fibrotic diseases. Despite being encoded by a single gene, alternative splicing of its pre-mRNA generates at least 20 different isoforms in humans. Fibronectin exists in two main forms: a soluble form found in body fluids and an insoluble form incorporated into extracellular matrices and basement membranes. In its soluble state, fibronectin adopts a compact structure due to intramolecular interactions. However, when incorporated into the ECM through a process known as fibronectin assembly, the protein unfolds into an extended conformation. Soluble fibronectin is primarily synthesized by hepatocytes and released into the bloodstream, while fibroblasts and endothelial cells are responsible for producing the insoluble, matrix-associated form.

References
https://pubmed.ncbi.nlm.nih.gov/31779172/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%