research use only

FDI-6 FOXM1 inhibitor

Name: FDI-6
Brand: Selleck Chemicals
SKU: S9689
Availability: InStock
Rating: 5 (4 reviews)

Cat.No.S9689

FDI-6 (NCGC00099374) is an inhibitor of the Forkhead box protein M1 transcription factor (FOXM1) with IC50 of 22.5 μM.

Chemical Structure

Molecular Weight: 437.43

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Quality Control

Batch: Purity: 100.00%

100.00

Related Targets	CDK HSP K-Ras PD-1/PD-L1 ROCK Wee1 DNA/RNA Synthesis Microtubule Associated Ras Aurora Kinase
Other FOXM1 Products	RCM-1 FoxM1 Antibody [K19K15]

Solubility

In vitro
Batch:

DMSO : 87 mg/mL (198.88 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.420mg/ml (0.96mM)	Taking the 1 mL working solution as an example, add 50 μL 8.3 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	437.43	Formula	C₁₉H₁₁F₄N₃OS₂	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	313380-27-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NCGC00099374			Smiles	NC1=C(SC2=NC(=CC(=C12)C(F)(F)F)C3=CC=CS3)C(=O)NC4=CC=C(F)C=C4

Mechanism of Action

Targets/IC₅₀/Ki	FOXM1 (Cell-free assay) 22.5 μM
In vitro	FDI-6 (NCGC00099374) is characterized in depth and is shown to bind directly to FOXM1 protein, to displace this compound from genomic targets in MCF-7 breast cancer cells, and induces concomitant transcriptional down-regulation. Global transcript profiling of MCF-7 cells by RNA-seq shows that this chemical specifically down-regulates FOXM1-activated genes with FOXM1 occupancy confirmed by ChIP-seq.
In vivo	Inhibiting FOXM1 with FDI-6 inhibits liver cancer cell growth in vivo.
References	[1] https://pubmed.ncbi.nlm.nih.gov/25387393/ [2] https://pubmed.ncbi.nlm.nih.gov/32080887/

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Handling Instructions

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