Home Cell Cycle FOXM1 inhibitor FDI-6 For research use only.

FDI-6

Synonyms: NCGC00099374

FDI-6 (NCGC00099374) is an inhibitor of the Forkhead box protein M1 transcription factor (FOXM1) with IC50 of 22.5 μM.

FDI-6 Chemical Structure

FDI-6 Chemical Structure

CAS: 313380-27-7

Selleck's FDI-6 has been cited by 2 publications

Purity & Quality Control

Batch: S968901 DMSO] 87 mg/mL] false] Water] Insoluble] false] Ethanol] Insoluble] false Purity: 99.75%
99.75

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Biological Activity

Description FDI-6 (NCGC00099374) is an inhibitor of the Forkhead box protein M1 transcription factor (FOXM1) with IC50 of 22.5 μM.
Targets
FOXM1 [1]
(Cell-free assay)
22.5 μM
In vitro
In vitro

FDI-6 (NCGC00099374) is characterized in depth and is shown to bind directly to FOXM1 protein, to displace FOXM1 from genomic targets in MCF-7 breast cancer cells, and induces concomitant transcriptional down-regulation. Global transcript profiling of MCF-7 cells by RNA-seq shows that FDI-6 specifically down-regulates FOXM1-activated genes with FOXM1 occupancy confirmed by ChIP-seq.[1]
Cell Research Cell lines MCF-7 cells
Concentrations 20 μM, 40 μM
Incubation Time 6 h
Method

MCF-7 cells are grown in 15 cm2 dishes to 70% confluence prior to FDI-6 or DMSO control treatment for 6h. Cells are washed with PBS, cross-linked with 1% formaldehyde at RT for 10 min and quenched with 1/20 volume glycine (2.5 M). Cells are harvested by scraping into cold PBS containing protease inhibitors and cell pellets stored at −80 ℃.
In Vivo
In vivo

Inhibiting FOXM1 with FDI-6 inhibits liver cancer cell growth in vivo.[2]
Animal Research Animal Models 4-week old male nude mice
Dosages 25 mg/kg
Administration intratumoral injection

Chemical Information & Solubility

Molecular Weight 437.43 Formula

C19H11F4N3OS2
CAS No. 313380-27-7 SDF --
Smiles NC1=C(SC2=NC(=CC(=C12)C(F)(F)F)C3=CC=CS3)C(=O)NC4=CC=C(F)C=C4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 87 mg/mL ( (198.88 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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