FDI-6

For research use only.

Catalog No.S9689 Synonyms: NCGC00099374

1 publication

FDI-6 Chemical Structure

CAS No. 313380-27-7

FDI-6 (NCGC00099374) is an inhibitor of the Forkhead box protein M1 transcription factor (FOXM1) with IC50 of 22.5 μM.

Selleck's FDI-6 has been cited by 1 publication

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Biological Activity

Description FDI-6 (NCGC00099374) is an inhibitor of the Forkhead box protein M1 transcription factor (FOXM1) with IC50 of 22.5 μM.
Targets
FOXM1 [1]
(Cell-free assay)
22.5 μM
In vitro

FDI-6 (NCGC00099374) is characterized in depth and is shown to bind directly to FOXM1 protein, to displace FOXM1 from genomic targets in MCF-7 breast cancer cells, and induces concomitant transcriptional down-regulation. Global transcript profiling of MCF-7 cells by RNA-seq shows that FDI-6 specifically down-regulates FOXM1-activated genes with FOXM1 occupancy confirmed by ChIP-seq.[1]

In vivo

Inhibiting FOXM1 with FDI-6 inhibits liver cancer cell growth in vivo.[2]

Protocol

Cell Research:

[1]

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  • Cell lines: MCF-7 cells
  • Concentrations: 20 μM, 40 μM
  • Incubation Time: 6 h
  • Method:

    MCF-7 cells are grown in 15 cm2 dishes to 70% confluence prior to FDI-6 or DMSO control treatment for 6h. Cells are washed with PBS, cross-linked with 1% formaldehyde at RT for 10 min and quenched with 1/20 volume glycine (2.5 M). Cells are harvested by scraping into cold PBS containing protease inhibitors and cell pellets stored at −80 ℃.


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: 4-week old male nude mice
  • Dosages: 25 mg/kg
  • Administration: intratumoral injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 87 mg/mL (198.88 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 437.43
Formula

C19H11F4N3OS2

CAS No. 313380-27-7
Storage powder
in solvent
Synonyms NCGC00099374

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID