Choose Your Country or Region

EPF Rabbit mAb

Catalog No.: F3797

Application: Reactivity: Mouse, Human

Usage Information

Dilution
WB1:10000 - 1:50000 IHC1:250 - 1:500 IF1:100 - 1:250

Application
WB, IHC, IF

Reactivity
Mouse, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
11 kDa 11 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
EPF Rabbit mAb detects endogenous levels of total EPF protein.

Clone
A15M14

Synonym(s)
Hsp10, 10 kDa chaperonin, Chaperonin 10, Early-pregnancy factor, Heat shock protein family E member 1, CPN10, EPF, HSPE1

Background
Chaperonin 10 (cpn10) is a member of the heat shock protein (hsp) family that functions as a mitochondrial molecular chaperone and contributes to cellular protection under stress conditions. Early pregnancy factor (EPF), detectable in the serum of all pregnant mammals shortly after conception, possesses both immunomodulatory and growth-promoting properties and was later identified as homologous to cpn10. Loss of mitochondrial integrity and function activates the mitochondrial unfolded protein response (UPRmt), a transcriptional program that restores proteostasis. The 10 kDa mitochondrial heat shock protein (HSP10), also known as heat shock protein family E member 1 (HSPE1), serves as a mitochondria-specific chaperone and plays a key role in this pathway. Experimental studies have shown that depletion of Akt3 in primary endothelial cells induces mitochondrial stress, which results in increased expression of HSP10 along with HSP60, another essential mitochondrial chaperone. Importantly, HSP10 and HSP60 are among the most abundant proteins within mitochondria. Beyond its mitochondrial functions, EPF/cpn10 exerts immunomodulatory effects, enhances cell survival, and suppresses toll-like receptor activation by lipopolysaccharide.

Background

Chaperonin 10 (cpn10) is a member of the heat shock protein (hsp) family that functions as a mitochondrial molecular chaperone and contributes to cellular protection under stress conditions. Early pregnancy factor (EPF), detectable in the serum of all pregnant mammals shortly after conception, possesses both immunomodulatory and growth-promoting properties and was later identified as homologous to cpn10. Loss of mitochondrial integrity and function activates the mitochondrial unfolded protein response (UPRmt), a transcriptional program that restores proteostasis. The 10 kDa mitochondrial heat shock protein (HSP10), also known as heat shock protein family E member 1 (HSPE1), serves as a mitochondria-specific chaperone and plays a key role in this pathway. Experimental studies have shown that depletion of Akt3 in primary endothelial cells induces mitochondrial stress, which results in increased expression of HSP10 along with HSP60, another essential mitochondrial chaperone. Importantly, HSP10 and HSP60 are among the most abundant proteins within mitochondria. Beyond its mitochondrial functions, EPF/cpn10 exerts immunomodulatory effects, enhances cell survival, and suppresses toll-like receptor activation by lipopolysaccharide.

References
https://pubmed.ncbi.nlm.nih.gov/18465204/ https://pubmed.ncbi.nlm.nih.gov/33087445/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%