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eIF4A3 Rabbit mAb

Catalog No.: F3433

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 - 1:2000 IP1:30 IHC1:100 IF1:500 FCM1:160

Application
WB, IP, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
47 kDa 46 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
eIF4A3 Rabbit mAb detects endogenous levels of total eIF4A3 protein.

Clone
F13B8

Synonym(s)
DDX48, KIAA0111, EIF4A3, Eukaryotic initiation factor 4A-III, eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix protein 265, NMP 265, hNMP 265

Background
Eukaryotic initiation factor 4A-3 (EIF4A3), also known as hnRNP265 or DDX48, is a member of the DEAD-box helicase protein family, ubiquitously expressed in humans and widely distributed across eukaryotes. EIF4A3 is a nucleocytoplasmic shuttling protein present in both the nucleus and cytoplasm, entering the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. As a core component of the EJC, EIF4A3 plays a critical role in post-transcriptional gene regulation. It participates in splicing-dependent multiprotein EJC assembly to control precursor mRNA splicing, monitor mRNA quality before translation, and regulate RNA metabolism. This regulation influences nonsense-mediated mRNA decay and ensures proper RNA processing. EIF4A3 also maintains the expression of key selenoproteins, including phospholipid hydroperoxide glutathione peroxidase and thioredoxin reductase 1. EIF4A3 interacts with multiple proteins, including other core EJC components, and can be recruited by long non-coding RNAs (lncRNAs) to regulate protein expression. In cancer biology, EIF4A3 is frequently overexpressed and contributes to tumorigenesis by stabilizing specific proteins. Elevated levels of EIF4A3 have been reported in several human cancers, including glioblastoma, hepatocellular carcinoma, pancreatic cancer, and ovarian cancer, where it promotes tumor growth.

Background

Eukaryotic initiation factor 4A-3 (EIF4A3), also known as hnRNP265 or DDX48, is a member of the DEAD-box helicase protein family, ubiquitously expressed in humans and widely distributed across eukaryotes. EIF4A3 is a nucleocytoplasmic shuttling protein present in both the nucleus and cytoplasm, entering the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. As a core component of the EJC, EIF4A3 plays a critical role in post-transcriptional gene regulation. It participates in splicing-dependent multiprotein EJC assembly to control precursor mRNA splicing, monitor mRNA quality before translation, and regulate RNA metabolism. This regulation influences nonsense-mediated mRNA decay and ensures proper RNA processing. EIF4A3 also maintains the expression of key selenoproteins, including phospholipid hydroperoxide glutathione peroxidase and thioredoxin reductase 1. EIF4A3 interacts with multiple proteins, including other core EJC components, and can be recruited by long non-coding RNAs (lncRNAs) to regulate protein expression. In cancer biology, EIF4A3 is frequently overexpressed and contributes to tumorigenesis by stabilizing specific proteins. Elevated levels of EIF4A3 have been reported in several human cancers, including glioblastoma, hepatocellular carcinoma, pancreatic cancer, and ovarian cancer, where it promotes tumor growth.

References
https://pubmed.ncbi.nlm.nih.gov/34458150/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%