E2F6 Rabbit mAb

Catalog No.: F3633

    Application: Reactivity:

    Usage Information

    Dilution
    1:1000
    1:30
    1:5000
    1:100
    1:500
    Application
    WB, IP, IHC, IF, FCM
    Reactivity
    Human, Mouse, Rat
    Source
    Rabbit
    Storage Buffer
    PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3
    Storage (from the date of receipt)
    -20°C (avoid freeze-thaw cycles), 2 years
    Predicted MW Observed MW
    32 kDa 35 kDa
    *Why do the predicted and actual molecular weights differ?
    The following reasons may explain differences between the predicted and actual protein molecular weight.

    Datasheet & SDS

    Biological Description

    Specificity
    E2F6 Rabbit mAb detects endogenous levels of total E2F6 protein.
    Clone
    P14C24
    Synonym(s)
    Transcription factor E2F6, E2F-6, E2F6
    Background
    E2F6 (E2F transcription factor 6) is an atypical member of the E2F transcription factor family that primarily acts as a transcriptional repressor rather than an activator. Unlike other E2F proteins, E2F6 lacks a transactivation domain and does not bind the retinoblastoma protein (pRB), making it functionally distinct. It contains a conserved DNA-binding domain and a dimerization domain that allow it to bind E2F target gene promoters, but its N-terminal region is unique and lacks activation sequences. E2F6 contains a repression domain within the marked-box region (residues 129–240) that recruits polycomb group proteins, including RYBP, Bmi1, and Ring1, forming repressive chromatin-modifying complexes. Through these interactions, E2F6 silences E2F-responsive genes involved in cell cycle progression, particularly at the G1/S phase transition. It also represses genes related to differentiation and development, contributing to chromatin state regulation and maintenance of cellular identity. E2F6 plays important roles in controlling proliferation, apoptosis, and developmental gene regulation. Its dysregulation can disrupt normal cell cycle control and is associated with developmental abnormalities and cancer.
    References
    • https://pubmed.ncbi.nlm.nih.gov/18366140/
    • https://pubmed.ncbi.nlm.nih.gov/11171983/

    Tech Support

    Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

    Handling Instructions

    Tel: +1-832-582-8158 Ext:3
    If you have any other enquiries, please leave a message.

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