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DLST (G11E23) Rabbit mAb

Catalog No.: F3701

Application: Reactivity: Human

Usage Information

Dilution
WB1:10000 - 1:50000 IP1:10 - 1:100 IHC1:100 - 1:250

Application
WB, IP, IHC

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
49 kDa

Datasheet & SDS

Biological Description

Specificity
DLST (G11E23) Rabbit mAb detects endogenous levels of total DLST protein.

Clone
G11E23

Synonym(s)
DLTS, DLST, 2-oxoglutarate dehydrogenase complex component E2, Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, E2K, OGDC-E2

Background
DLST (Dihydrolipoamide S-succinyltransferase) is the E2 core enzyme of the mitochondrial 2-oxoglutarate dehydrogenase complex (OGDHC), a key multienzyme complex in the tricarboxylic acid (TCA) cycle. DLST forms the multimeric scaffold that organizes the OGDHC components, including the E1 (2-oxoglutarate dehydrogenase) and E3 (dihydrolipoamide dehydrogenase) subunits. Each DLST monomer contains a lipoyl domain with a covalently attached lipoate group at lysine 110, enabling a swinging-arm mechanism that channels substrates between active sites. DLST catalyzes the transfer of a succinyl group from the lipoyl cofactor to coenzyme A, generating succinyl-CoA, an essential TCA intermediate critical for energy production and substrate-level phosphorylation. By facilitating oxidative decarboxylation of 2-oxoglutarate, DLST supports mitochondrial bioenergetics and links carbon metabolism to ATP synthesis. It also influences mitochondrial redox balance and reactive oxygen species (ROS) regulation. Disruption of DLST, due to mutations or altered expression, impairs OGDHC function and contributes to mitochondrial dysfunction observed in neurodegenerative diseases like Alzheimer's disease. Additionally, aberrant DLST activity contributes to metabolic reprogramming in cancer, promoting tumor growth and survival.

Background

DLST (Dihydrolipoamide S-succinyltransferase) is the E2 core enzyme of the mitochondrial 2-oxoglutarate dehydrogenase complex (OGDHC), a key multienzyme complex in the tricarboxylic acid (TCA) cycle. DLST forms the multimeric scaffold that organizes the OGDHC components, including the E1 (2-oxoglutarate dehydrogenase) and E3 (dihydrolipoamide dehydrogenase) subunits. Each DLST monomer contains a lipoyl domain with a covalently attached lipoate group at lysine 110, enabling a swinging-arm mechanism that channels substrates between active sites. DLST catalyzes the transfer of a succinyl group from the lipoyl cofactor to coenzyme A, generating succinyl-CoA, an essential TCA intermediate critical for energy production and substrate-level phosphorylation. By facilitating oxidative decarboxylation of 2-oxoglutarate, DLST supports mitochondrial bioenergetics and links carbon metabolism to ATP synthesis. It also influences mitochondrial redox balance and reactive oxygen species (ROS) regulation. Disruption of DLST, due to mutations or altered expression, impairs OGDHC function and contributes to mitochondrial dysfunction observed in neurodegenerative diseases like Alzheimer's disease. Additionally, aberrant DLST activity contributes to metabolic reprogramming in cancer, promoting tumor growth and survival.

References
https://pubmed.ncbi.nlm.nih.gov/8076640/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%