Dihydrotestosterone(DHT)

Catalog No.S4757 Synonyms: androstanolone, stanolone, 5α-DHT

Dihydrotestosterone(DHT) Chemical Structure

Molecular Weight(MW): 290.44

Dihydrotestosterone is an endogenous androgen sex steroid and hormone and a potent agonist of the androgen receptor with 2-3-fold greater androgen receptor affinity than testosterone and 10-fold higher potency of inducing androgen receptor signaling.

Size Price Stock Quantity  
USD 157 In stock
USD 370 In stock
Bulk Discount
Bulk Inquiry

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 1 publication

Purity & Quality Control

Choose Selective Androgen Receptor Inhibitors

Biological Activity

Description Dihydrotestosterone is an endogenous androgen sex steroid and hormone and a potent agonist of the androgen receptor with 2-3-fold greater androgen receptor affinity than testosterone and 10-fold higher potency of inducing androgen receptor signaling.
Targets
Androgen Receptor [1]
()
In vitro

In AR-positive bladder cancer UMUC3 and TCC-SUP cells, dihydrotestosterone (DHT) increased the expression of EGFR and ERBB2 both in mRNA and in protein levels. DHT additionally upregulated the levels of phosphorylation of EGFR (pEGFR) and its downstream proteins AKT (pAKT) and ERK1/2 (pERK), induced by EGF treatment, in AR-positive cells[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CV-1 cell NX\udVBwTnWwY4Tpc44h[XO|YYm= MljaRYdwdmm|dHnjJIFkfGm4aYT5JEhGSzVyKTDh[4FqdnO2IHj1cYFvKGGwZILv[4VvKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDWk0yKGOnbHysJGVEPTB;MD6wNFYh|ryP M17rSFk5PzF3M{S=
MDA-MB-453 cell M{mzZ2Z2dmO2aX;uJIF{e2G7 MU\EbZNxdGGlZX3lcpQhd2ZiW{PIYWRJXCCocn;tJIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBOTEFvTVKtOFU{KGOnbHzzJIJ6KHeqb3zlJINmdGxicnXj[ZB1d3JiYnnu[Ilv\yCjc4PhfUwhU2l;MD6wNFAzKM7:TR?= NHnlU|MyPzB|NEGxOy=>
HepG2 cell Ml;USpVv[3Srb36gZZN{[Xl? MVTBZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKGGwZILv[4VvKHKnY3XweI9zKGW6cILld5Nm\CCrbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[WO2aY\heIlwdixiSVO1NF0xNjBzOEKg{txO NWjlNmVmOTdzNEm4OlY>
MDA453 cell M4DYS2Z2dmO2aX;uJIF{e2G7 MWLEbZNxdGGlZX3lcpQhd2ZiW{PIYWRJXCCocn;tJIh2dWGwIFHSJIlvKE2GQUS1N{Bk\WyuczygT4k:OC5yMEOg{txO MmnDNVcyQDFzNEG=
mouse C2C12 cell MonDSpVv[3Srb36gZZN{[Xl? M3HGW2Fod26rc4SgZYN1cX[rdImgZZQhemG2IFHSJJRz[W6|ZnXjeIVlKGmwIH3veZNmKEN{Q{GyJINmdGy|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIhfHKjboPhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxRTBwMEK4JO69VQ>? MYKxO|E5OTF2MR?=
monkey COS7 cell NFHNXnVHfW6ldHnvckBie3OjeR?= Mn3iRolv\GmwZzDh[oZqdmm2eTD0c{BpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hdW:wa3X5JGNQWzdiY3XscJMh[nlid3jvcIUh[2WubDDibY5lcW6pIHHzd4F6NCCNaU2wMlAxODJizszN MnniNVg1PDJ7MUK=
CHO cell NGHZPFlHfW6ldHnvckBie3OjeR?= M{TUTGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gWGdTPSCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIJ6KGy3Y3nm[ZJie2ViYYPzZZktKEWFNUC9OE41QSEQvF2= NEHJN3cyQDNyN{K5OC=>
COS7 cell NX;JOWNGTnWwY4Tpc44h[XO|YYm= MonQRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDXO|QySyCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOgZZN{\XO|ZXSgZZMhdHWlaX\ldoF{\SCjY4Tpeol1gSCjZoTldkAzPCCqcoOgZpkhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjBzMjFOwG0> M2LieFIzODl2Mke5
COS7 cell M1SyWWZ2dmO2aX;uJIF{e2G7 M2DHfGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4KgWFg4P0FibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJIF{e2W|c3XkJIF{KGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMUWg{txO NIW0RYszOjB7NEK3PS=>
COS7 cell Ml\LSpVv[3Srb36gZZN{[Xl? MXvB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGG|c3Xzd4VlKGG|IHz1Z4ln\XKjc3WgZYN1cX[rdImgZYZ1\XJiMkSgbJJ{KGK7IILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6wNFYzKM7:TR?= MWqyNlA6PDJ5OR?=
LNCaP-hr cell NHj1ZlJHfW6ldHnvckBie3OjeR?= NFnBTIQ{KGSjeYO= M2m1TGFod26rc4SgZYN1cX[rdImgZZQhSW6mcn;n[Y4hemWlZYD0c5IhXDh5N1GgcZV1[W62IHnuJIh2dWGwIFzOR4FRNWi{IHPlcIx{KGG|c3Xzd4VlKGG|IIDyc5N1[XSnIIPw[YNq\mmlIHHueIlo\W5ic3XjdoV1cW:wIH3lZZN2emWmIHHmeIVzKDNiZHH5d{BjgSCnbor5cYUucW2vdX7vZZN{[XluIFXDOVA:OC5yMUWg{txO MVmyN|E6QTR5Nx?=
LNCaP-hr cell MX;GeY5kfGmxbjDhd5NigQ>? NI\sNnI{KGSjeYO= MnHGRYdwdmm|dDDhZ5Rqfmm2eTDheEB4cWymIIT5dIUhSW6mcn;n[Y4hemWlZYD0c5IhcW5iaIXtZY4hVE6FYWCtbJIh[2WubIOgZZN{\XO|ZXSgZZMheHKxc4TheIUhe3CnY3nmbYMh[W62aXflckB{\WO{ZYTpc44hdWWjc4Xy[YQh[W[2ZYKgN{Bl[Xm|IHL5JIVvgnmvZT3pcY12dm:jc4PhfUwhTUN3ME2wMlAxPjJizszN MVqyN|E6QTR5Nx?=
HEK293 cell NGrtbZVHfW6ldHnvckBie3OjeR?= MXHB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvOThibl2= NXrRVoVHOjV|MEW2PFg>

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western blot
Cyclin D1 / Cyclin D2 / Cyclin D3 / Cyclin E2 / Cyclin A1 / AR; 

PubMed: 26372468     


HPr-1AR cells were treated with 10 nM DHT or vehicle control for 24–48 hours (h), and immunoblot analysis of cell lysates reveals that cyclin D1 and D2 protein expression decreased substantially with DHT treatment compared to vehicle.

p-CDK2 / CDK2 / CDK4 / CDK6 / CDKN1A / p-RB / RB ; 

PubMed: 26372468     


HPr-1AR cells were treated with 10 nM DHT or vehicle control for 24–48 hours (h). Immunoblot analysis of cell lysates reveals that CDK4 and CDK6 protein expression decreased and CDKN1A expression increased with DHT treatment compared to vehicle. CDK2 phosphorylation at threonine 160 (T160) decreased nearly 50% with androgen treatment at 24 and 48 hours, albeit not significantly, and total CDK2 protein expression remained unchanged. Relative to total RB expression, the levels of various phosphorylated forms of RB (S780 and S807/811) decreased over time with androgen treatment. 

p27 / p-p27 ; 

PubMed: 22139837     


CWR22Rv1 or CWR22R3 cells were stimulated with 10 nm DHT for 6 h, with the addition of MG132 during the last 2 h before harvesting. Proteins were then immunoblotted.

AR / PP1 ; 

PubMed: 26636645     


HeLa cells were transfected with Flag-PP1α and HA-AR and then incubated for overnight in androgen-depleted medium without or with androgen (10 nM of DHT) as indicated for blotting. 

26372468 22139837 26636645
Immunofluorescence
FOXO1 / p27 ; 

PubMed: 22139837     


CWR22R3 cells cultured in medium with CDS serum were treated without/with DHT (10 nm) for 4 h. The cells were immunostained for FOXO1 and p27 and examined with a confocal microscope. 

AKT(S473) / PKCα(S657) ; 

PubMed: 22139837     


CWR22R3 cells cultured in medium with CDS serum were treated without/with 10 nm DHT (−/+ DHT) for 4 h. The cells were immunostained for AKT or PKCα phosphorylation and examined with a confocal microscope. Bars: 20 μm

22139837
In vivo DHT-treated SOD1-G93A mice demonstrate ameliorated muscle atrophy and increased body weight, which is associated with stronger grip-strength. DHT treatment increases the expression of insulin-like growth factor-1 in muscle, which can exert myotrophic as well as neurotrophic effects through retrograde transport. DHT treatment attenuates neuromuscular junction denervation, and axonal and motoneuron loss. DHT-treated SOD1-G93A mice demonstrates improvement in motor behavior as assessed by rota-rod and gait analyses, and an increased lifespan[2].

Protocol

Cell Research:

[1]
- Collapse
  • Cell lines: Bladder cancer cells
  • Concentrations: 1 nM
  • Incubation Time: 24 h
  • Method:

    Bladder cancer cells at a density of 50-60% confluence in 24-well plates are co-transfected with 250 ng of MMTV-luc reporter plasmid DNA and 2.5 ng of PRL-TK-luc plasmid DNA. After 6 h of transfection, the medium is replaced with another medium supplemented with charcoal-stripped FBS in the presence or absence of ligands (DHT, HF, or both) for 24 h. Cells were harvested, lysed, and assayed for luciferase activity.


    (Only for Reference)
Animal Research:

[2]
- Collapse
  • Animal Models: SOD1-G93A mutant mice with the congenic C57BL/6J background
  • Formulation: saline
  • Dosages: 5 mg
  • Administration: silastic implant
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 58 mg/mL (199.69 mM)
Ethanol 58 mg/mL (199.69 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 290.44
Formula

C19H30O2
CAS No. 521-18-6
Storage powder
in solvent
Synonyms androstanolone, stanolone, 5α-DHT

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04032067 Not yet recruiting Drug: GV1001 placebo|Drug: Proscar placebo Benign Prostatic Hyperplasia (BPH) GemVax & Kael August 28 2019 Phase 3
NCT03361969 Recruiting Drug: Estetrol|Drug: Placebo Oral Tablet Prostatic Neoplasm Pantarhei Oncology B.V. April 16 2018 Phase 2
NCT03314298 Completed Drug: testosterone anastrozole Mammographic Density Havah Therapeutics Pty Ltd August 14 2017 Phase 1
NCT02574910 Recruiting Drug: Abiraterone acetate Congenital Adrenal Hyperplasia University of Texas Southwestern Medical Center|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)|University of Michigan|Children''s Hospital Los Angeles August 1 2017 Phase 1
NCT03085095 Recruiting Drug: Relugolix|Drug: Leuprolide Acetate Prostate Cancer Myovant Sciences GmbH April 18 2017 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field
selleck catalog

Androgen Receptor Signaling Pathway Map

Related Androgen Receptor Products

  • Darolutamide (ODM-201) New

    Darolutamide (ODM-201) is a novel androgen receptor (AR) antagonist that blocks AR nuclear translocation with Ki of 11 nM. Phase 3.

  • Sacubitril/valsartan (LCZ696) New

    Sacubitril/valsartan (LCZ696), consisting of valsartan and sacubitril in 1:1 molar ratio, is an orally bioavailable, dual-acting angiotensin receptor-neprilysin inhibitor (ARNi) for hypertension and heart failure. Phase 3.

  • Endoxifen HCl New

    Endoxifen HCl, the active metabolite of Tamoxifen, ia a potent and selective estrogen receptor antagonist. Phase 2.

  • Enzalutamide (MDV3100)

    Enzalutamide (MDV3100) is an androgen-receptor (AR) antagonist with IC50 of 36 nM in LNCaP cells.

  • Bicalutamide

    Bicalutamide is an androgen receptor (AR) antagonist with IC50 of 0.16 μM in LNCaP/AR(cs)cell line.

  • Apalutamide (ARN-509)

    Apalutamide (ARN-509) is a selective and competitive androgen receptor inhibitor with IC50 of 16 nM in a cell-free assay, useful for prostate cancer treatment. Phase 3.

  • Dehydroepiandrosterone (DHEA)

    Dehydroepiandrosterone is an important endogenous steroid hormone, which is an androgen receptor antagonist and an estrogen receptor agonist.

  • Galeterone

    Galeterone is a selective CYP17 inhibitor and androgen receptor (AR) antagonist with IC50 of 300 nM and 384 nM, respectively, and is a potent inhibitor of human prostate tumor growth. Phase 2.

  • Ostarine (GTx-024, MK-2866 )

    MK-2866 (GTx-024) is a selective androgen receptor modulator (SARM) with Ki of 3.8 nM, and is tissue-selective for anabolic organs. Phase 3.

    Features:The most potent and tissue-selective in vivo activity of SARMs to date, with favorable pharmacokinetic properties.

  • Flutamide

    Flutamide is an antiandrogen drug, with its active metablolite binding at androgen receptor with Ki values of 55 nM, and primarily used to treat prostate cancer.

Tags: buy Dihydrotestosterone(DHT) | Dihydrotestosterone(DHT) supplier | purchase Dihydrotestosterone(DHT) | Dihydrotestosterone(DHT) cost | Dihydrotestosterone(DHT) manufacturer | order Dihydrotestosterone(DHT) | Dihydrotestosterone(DHT) distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID