Dihydrotestosterone (DHT)

For research use only.

Catalog No.S4757 Synonyms: androstanolone, stanolone, 5α-DHT

6 publications

Dihydrotestosterone (DHT) Chemical Structure

CAS No. 521-18-6

Dihydrotestosterone (DHT, androstanolone, stanolone, 5α-DHT) is an endogenous androgen sex steroid and hormone and a potent agonist of the androgen receptor with 2-3-fold greater androgen receptor affinity than testosterone and 10-fold higher potency of inducing androgen receptor signaling.

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10mM (1mL in DMSO) RMB 1367.81 In stock
RMB 1286.81 In stock
RMB 3030.86 In stock
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Selleck's Dihydrotestosterone (DHT) has been cited by 6 publications

Purity & Quality Control

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Biological Activity

Description Dihydrotestosterone (DHT, androstanolone, stanolone, 5α-DHT) is an endogenous androgen sex steroid and hormone and a potent agonist of the androgen receptor with 2-3-fold greater androgen receptor affinity than testosterone and 10-fold higher potency of inducing androgen receptor signaling.
Targets
Androgen Receptor [1]
()
In vitro

In AR-positive bladder cancer UMUC3 and TCC-SUP cells, dihydrotestosterone (DHT) increased the expression of EGFR and ERBB2 both in mRNA and in protein levels. DHT additionally upregulated the levels of phosphorylation of EGFR (pEGFR) and its downstream proteins AKT (pAKT) and ERK1/2 (pERK), induced by EGF treatment, in AR-positive cells[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CV-1 cell NXrNWXpPTnWwY4Tpc44h[XO|YYm= NUXzPXl{SWexbnnzeIlkKGGldHn2bZR6KCiHQ{WwLUBi\2GrboP0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEXi1zIHPlcIwtKEWFNUC9NE4xODZizszN M{HOSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl6N{G1N|QoRjl6N{G1N|Q9N2F-
MDA-MB-453 cell MXfGeY5kfGmxbjDhd5NigQ>? NE\XdpNFcXOybHHj[Y1mdnRib3[gX|NJZUSKVDDmdo9uKGi3bXHuJIFv\HKxZ3XuJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCPRFGtUWIuPDV|IHPlcIx{KGK7IIfoc4xmKGOnbHygdoVk\XC2b4KgZolv\GmwZzDhd5NigSxiS3m9NE4xODB{IN88US=> Ml;sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyM{SxNVcoRjF5MEO0NVE4RC:jPh?=
HepG2 cell NWriW3VFTnWwY4Tpc44h[XO|YYm= NF7peVdC[3Srdnn0fUBi\2GrboP0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJ\XCJMjDj[YxteyCjc4Pld5Nm\CCjczDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYXP0bZZifGmxbjygTWM2OD1yLkCxPFIh|ryP MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF2OUi2Okc,OTdzNEm4OlY9N2F-
MDA453 cell MXrGeY5kfGmxbjDhd5NigQ>? M37WT2Rqe3CuYXPlcYVvfCCxZjDbN2heTEiWIH\yc40hcHWvYX6gRXIhcW5iTVTBOFU{KGOnbHzzMEBMcT1yLkCwN{DPxE1? MmC0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzOEGxOFEoRjF5MUixNVQyRC:jPh?=
mouse C2C12 cell MYnGeY5kfGmxbjDhd5NigQ>? MUnB[49vcXO2IHHjeIl3cXS7IHH0JJJifCCDUjD0doFve2[nY4Tl[EBqdiCvb4Xz[UBEOkNzMjDj[YxteyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NF0xNjB{ODFOwG0> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF6MUG0NUc,OTdzOEGxOFE9N2F-
monkey COS7 cell NIPZW|NHfW6ldHnvckBie3OjeR?= M3jPdWJqdmSrbnegZYZncW6rdImgeI8hcHWvYX6gZY5lem:pZX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKG2xbnvlfUBEV1N5IHPlcIx{KGK7IIfoc4xmKGOnbHygZolv\GmwZzDhd5NigSxiS3m9NE4xODB{IN88US=> NW\PZoNWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0OFI6OTJpPkG4OFQzQTF{PD;hQi=>
CHO cell M2OzO2Z2dmO2aX;uJIF{e2G7 MnT0RYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDUS3I2KGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZpkhdHWlaX\ldoF{\SCjc4PhfUwhTUN3ME20MlQ6KM7:TR?= M4f5O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{C3Nlk1Lz5zOEOwO|I6PDxxYU6=
COS7 cell NFPBRY1HfW6ldHnvckBie3OjeR?= Mn\XRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDXO|QySyCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOgZZN{\XO|ZXSgZZMhdHWlaX\ldoF{\SCjY4Tpeol1gSCjZoTldkAzPCCqcoOgZpkhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjBzMjFOwG0> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB7NEK3PUc,OjJyOUSyO|k9N2F-
COS7 cell MY\GeY5kfGmxbjDhd5NigQ>? M{XCbGFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4KgWFg4P0FibYX0ZY51KGW6cILld5Nm\CCrbjDDU3M4KGOnbHzzJIF{e2W|c3XkJIF{KGy3Y3nm[ZJie2ViYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5yMUWg{txO NIrXfoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC5OFI4QSd-MkKwPVQzPzl:L3G+
COS7 cell NUnVZ4xtTnWwY4Tpc44h[XO|YYm= NGjSZo9C\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJIFv\HKxZ3XuJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFT2O3JINmdGy|IHHzd4V{e2WmIHHzJIx2[2moZYLhd4Uh[WO2aY\peJkh[W[2ZYKgNlQhcHK|IHL5JJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9NE4xODZ{IN88US=> NUS1TVNLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwPVQzPzlpPkKyNFk1Ojd7PD;hQi=>
LNCaP-hr cell MWPGeY5kfGmxbjDhd5NigQ>? NFTTWZE{KGSjeYO= NIntWGlC\2:waYP0JIFkfGm4aYT5JIF1KEGwZILv[4VvKHKnY3XweI9zKFR6N{fBJI12fGGwdDDpckBpfW2jbjDMUmNiWC2qcjDj[YxteyCjc4Pld5Nm\CCjczDwdo9{fGG2ZTDzdIVkcW[rYzDhcpRq\2WwIIPlZ5JmfGmxbjDt[YF{fXKnZDDh[pRmeiB|IHThfZMh[nliZX76fY1mNWmvbYXuc4F{e2G7LDDFR|UxRTBwMEG1JO69VQ>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF7OUS3O{c,OjNzOUm0O|c9N2F-
LNCaP-hr cell NGnsS5lHfW6ldHnvckBie3OjeR?= NFn2OXc{KGSjeYO= M4PY[mFod26rc4SgZYN1cX[rdImgZZQhf2muZDD0fZBmKEGwZILv[4VvKHKnY3XweI9zKGmwIHj1cYFvKEyQQ3HQMYhzKGOnbHzzJIF{e2W|c3XkJIF{KHC{b4P0ZZRmKHOyZXPp[olkKGGwdHnn[Y4he2WlcnX0bY9vKG2nYYP1doVlKGGodHXyJFMh\GG7czDifUBmdnq7bXWtbY1ufW6xYYPzZZktKEWFNUC9NE4xODZ{IN88US=> NHLJb5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G5PVQ4Pyd-MkOxPVk1Pzd:L3G+
HEK293 cell MXLGeY5kfGmxbjDhd5NigQ>? M4jiNmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5zODDuUS=> MmDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|MEW2PFgoRjJ3M{C1Olg5RC:jPh?=
COS MoHHSpVv[3Srb36gZZN{[Xl? M{jLXGJqdmSrbnegZYZncW6rdImgZYdicW6|dDDBcoRzd2enbjDy[YNmeHSxcjDlfJBz\XO|ZXSgR29UKGOnbHzzMEBMcSB;IECuNFAzKM7:TT6= M2L6RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl6N{G1N|QoRjl6N{G1N|Q9N2F-
COS-1 MYjGeY5kfGmxbjDhd5NigQ>? NUWyXpVzSmmwZHnu[{Bi\m[rbnn0fUBnd3JiaIXtZY4hSW6mcn;n[Y4hemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPPV{0yKGOnbHzzMEBMcSB;IECuNFA{KM7:TT6= NGnQUGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97OUK1O|I2Lz57OUK1O|I2RC:jPh?=
CV-1 MnjCSpVv[3Srb36gZZN{[Xl? MlewRYdwdmm|dDDhZ5Rqfmm2eTDh[4FqdnO2IFj1cYFvKEGwZILv[4VvKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDWk0yKGOnbHzzMEBGSzVyIE2gNE4xODVizszNMi=> MorKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTl{NUeyOUc,QTl{NUeyOVww[T5?
COS M165eGZ2dmO2aX;uJIF{e2G7 NHHPcFJKdiC4aYTyc{BjcW6maX7nJIFn\mmwaYT5JIF1KGi3bXHuJIFv\HKxZ3XuJJJm[2WydH;yJJRz[W6|ZnXjeIVlKGmwdH:gR29UKGOnbHzzMkwhUUN3MDC9JFAvODB2IN88UU4> MmX4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB{M{C2NlgoRjFyMkOwOlI5RC:jPh?=
CV-1 MkP0SpVv[3Srb36gZZN{[Xl? NX\CNpFDUW5idnn0do8h[WexbnnzeIlkKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4KgeZNqdmdiY3;0doFve2[nY4Tpc44h[XO|YYmgbY4hS1ZvMTDj[Yxtey5uIFXDOVAhRSByLkCwOkDPxE1w NE\PcGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEKzNFYzQCd-MUCyN|A3Ojh:L3G+
CV-1 NEH1dW9HfW6ldHnvckBie3OjeR?= Ml3KRYdwdmm|dDDhZ5Rqfmm2eTD0c{B1cGViaIXtZY4h[W6mcn;n[Y4hemWlZYD0c5IhMGiDUjmgbY4hS1ZvMTDj[YxteyxiRVO1NEA:KDBwMEC2JO69VS5? NGO1Vno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEO0NFYzPCd-MUCzOFA3OjR:L3G+
CV1 NVnSZWRKTnWwY4Tpc44h[XO|YYm= MmDVRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDpckBEXjFiY3XscJMhdWWjc4Xy[YQh[XNic4TpcZVt[XSrb36gc4YhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIFkfGm4aYT5JIJ6KGOxdILhcpNn\WO2aX;uJIF{e2G7LDDFR|UxKD1iMD6wNFUyKM7:TT6= MorqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdyM{SxNVcoRjF5MEO0NVE4RC:jPh?=
CV1 M2PROmZ2dmO2aX;uJIF{e2G7 MUnCbY5lcW6pIHHm[olvcXS7IITvJIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEXjFiY3XscJMtKEurIE2gNE4xODB{IN88UU4> NHzPPY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{K1O|g{QCd-MUeyOVc5Ozh:L3G+
CV1 MnzoSpVv[3Srb36gZZN{[Xl? MVLB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEXjFiY3XscJMtKEWFNUCgQUAxNjByNUGg{txONg>? M3;yOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MkW3PFM5Lz5zN{K1O|g{QDxxYU6=
MDA-MB-453 MWLGeY5kfGmxbjDhd5NigQ>? NX32[GRETGm|cHzhZ4Vu\W62IH;mJHs{UF2GSGSg[pJwdSCqdX3hckBCWiCneIDy[ZN{\WRiaX6gUWRCNU2ELUS1N{Bk\WyuczygT4khRSByLkCwNFMh|ryPLh?= M{PUNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEO5NVEzLz5zN{SzPVEyOjxxYU6=
CV1 NFewOolHfW6ldHnvckBie3OjeR?= MXTB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEXjFiY3XscJMh[nlidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjByNUeg{txONg>? NV6wRpdlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0N|kyOTJpPkG3OFM6OTF{PD;hQi=>
CV1 NUm2TJBZTnWwY4Tpc44h[XO|YYm= NH;x[JpC\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJIFv\HKxZ3XuJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFVkGgZ4VtdHNiYomgeJJidnOlcnnweIlwdmGuIHHjeIl3[XSrb36gZZN{[XluIFXDOVAhRSByLkCwOVch|ryPLh?= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzdyM{mzPEc,OTd5MEO5N|g9N2F-
MDA-MB-453 M3LZd2Z2dmO2aX;uJIF{e2G7 M{iwW2Rqe3CuYXPlcYVvfCCxZjDbN2heTEiWIH\yc40hcHWvYX6gRY5lem:pZX6gdoVk\XC2b4KgbY4hcHWvYX6gUWRCNU2ELUS1N{Bk\WyuczygT4khRSByLkCwNFkh|ryPLh?= NWTydId7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0NFA1QTlpPkG4OFAxPDl7PD;hQi=>
CV1 M1HuOWZ2dmO2aX;uJIF{e2G7 MlK2RYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDpckBEXjFiY3XscJMh[nlidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjByNUeg{txONg>? NH;ycpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOESwNFQ6QSd-MUi0NFA1QTl:L3G+
Saos2 M3W0dWZ2dmO2aX;uJIF{e2G7 M4T2NWFkfGm4aYT5JIF1KGGwZILv[4VvKHKnY3XweI9zKGmwIHj1cYFvKFOjb4OyJINmdGy|IHHzd4V{e2WmIHHzJGlNPiC{ZYDy[ZN{cW:wLDDJR|UxKD1iMD6wNFAxPSEQvF2u MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR2MkmxNkc,OTh2NEK5NVI9N2F-
CV1 MWXGeY5kfGmxbjDhd5NigQ>? Moq5RYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDh[pJq[2GwIHfy[YVvKG2xbnvlfUBEXjFiY3XscJMh[nliY3;0doFve2[nY4Tpc44h[XO|YYmsJGVEPTBiPTCwMlAxPTFizszNMi=> M3rkclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NESyPVEzLz5zOES0NlkyOjxxYU6=
MDA-kb2 M3nTPGZ2dmO2aX;uJIF{e2G7 NXTJZ2pSSWexbnnzeEBi[3Srdnn0fUBifCCjbnTyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCPRFGtb4IzKGOnbHzzJIF{e2W|c3XkJIF{KHO2aX31cIF1cW:wIH;mJIx2[2moZYLhd4Uh[WO2aY\peJkh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzF3MDC9JFAvODByNTFOwG0v NEHxOlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW5NlI1PSd-MUm1PVIzPDV:L3G+
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cyclin D1 / Cyclin D2 / Cyclin D3 / Cyclin E2 / Cyclin A1 / AR; 

PubMed: 26372468     


HPr-1AR cells were treated with 10 nM DHT or vehicle control for 24–48 hours (h), and immunoblot analysis of cell lysates reveals that cyclin D1 and D2 protein expression decreased substantially with DHT treatment compared to vehicle.

p-CDK2 / CDK2 / CDK4 / CDK6 / CDKN1A / p-RB / RB ; 

PubMed: 26372468     


HPr-1AR cells were treated with 10 nM DHT or vehicle control for 24–48 hours (h). Immunoblot analysis of cell lysates reveals that CDK4 and CDK6 protein expression decreased and CDKN1A expression increased with DHT treatment compared to vehicle. CDK2 phosphorylation at threonine 160 (T160) decreased nearly 50% with androgen treatment at 24 and 48 hours, albeit not significantly, and total CDK2 protein expression remained unchanged. Relative to total RB expression, the levels of various phosphorylated forms of RB (S780 and S807/811) decreased over time with androgen treatment. 

p27 / p-p27 ; 

PubMed: 22139837     


CWR22Rv1 or CWR22R3 cells were stimulated with 10 nm DHT for 6 h, with the addition of MG132 during the last 2 h before harvesting. Proteins were then immunoblotted.

AR / PP1 ; 

PubMed: 26636645     


HeLa cells were transfected with Flag-PP1α and HA-AR and then incubated for overnight in androgen-depleted medium without or with androgen (10 nM of DHT) as indicated for blotting. 

26372468 22139837 26636645
Immunofluorescence
FOXO1 / p27 ; 

PubMed: 22139837     


CWR22R3 cells cultured in medium with CDS serum were treated without/with DHT (10 nm) for 4 h. The cells were immunostained for FOXO1 and p27 and examined with a confocal microscope. 

AKT(S473) / PKCα(S657) ; 

PubMed: 22139837     


CWR22R3 cells cultured in medium with CDS serum were treated without/with 10 nm DHT (−/+ DHT) for 4 h. The cells were immunostained for AKT or PKCα phosphorylation and examined with a confocal microscope. Bars: 20 μm

22139837
In vivo DHT-treated SOD1-G93A mice demonstrate ameliorated muscle atrophy and increased body weight, which is associated with stronger grip-strength. DHT treatment increases the expression of insulin-like growth factor-1 in muscle, which can exert myotrophic as well as neurotrophic effects through retrograde transport. DHT treatment attenuates neuromuscular junction denervation, and axonal and motoneuron loss. DHT-treated SOD1-G93A mice demonstrates improvement in motor behavior as assessed by rota-rod and gait analyses, and an increased lifespan[2].

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: Bladder cancer cells
  • Concentrations: 1 nM
  • Incubation Time: 24 h
  • Method:

    Bladder cancer cells at a density of 50-60% confluence in 24-well plates are co-transfected with 250 ng of MMTV-luc reporter plasmid DNA and 2.5 ng of PRL-TK-luc plasmid DNA. After 6 h of transfection, the medium is replaced with another medium supplemented with charcoal-stripped FBS in the presence or absence of ligands (DHT, HF, or both) for 24 h. Cells were harvested, lysed, and assayed for luciferase activity.


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: SOD1-G93A mutant mice with the congenic C57BL/6J background
  • Dosages: 5 mg
  • Administration: silastic implant
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 58 mg/mL (199.69 mM)
Ethanol 58 mg/mL (199.69 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 290.44
Formula

C19H30O2

CAS No. 521-18-6
Storage powder
in solvent
Synonyms androstanolone, stanolone, 5α-DHT
Smiles CC12CCC(=O)CC1CCC3C2CCC4(C3CCC4O)C

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Bio Calculators

Molarity Calculator

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Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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Dilution Calculator

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01724619 Terminated Drug: [F-18] fluorinated dihydrotestosterone (FDHT) Prostate Cancer Wake Forest University Health Sciences November 2012 Early Phase 1
NCT00490022 Completed Drug: Dihydrotestosterone (DHT) gel (0.7%)|Drug: Placebo gel Healthy University of Washington|National Institute on Aging (NIA)|ASCEND Therapeutics June 2007 Phase 1|Phase 2
NCT00062790 Completed Drug: Dutasteride Prostatic Hyperplasia GlaxoSmithKline October 2003 Phase 4

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Androgen Receptor Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID