Shipping services continue despite COVID-19 outbreak ! Click here to view COVID-19 related products

Dihydrotestosterone (DHT)

For research use only.

Catalog No.S4757 Synonyms: androstanolone, stanolone, 5α-DHT

5 publications

Dihydrotestosterone (DHT) Chemical Structure

CAS No. 521-18-6

Dihydrotestosterone (DHT, androstanolone, stanolone, 5α-DHT) is an endogenous androgen sex steroid and hormone and a potent agonist of the androgen receptor with 2-3-fold greater androgen receptor affinity than testosterone and 10-fold higher potency of inducing androgen receptor signaling.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 99 In stock
USD 157 In stock
USD 370 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Dihydrotestosterone (DHT) has been cited by 5 publications

Purity & Quality Control

Choose Selective Androgen Receptor Inhibitors

Biological Activity

Description Dihydrotestosterone (DHT, androstanolone, stanolone, 5α-DHT) is an endogenous androgen sex steroid and hormone and a potent agonist of the androgen receptor with 2-3-fold greater androgen receptor affinity than testosterone and 10-fold higher potency of inducing androgen receptor signaling.
Targets
Androgen Receptor [1]
()
In vitro

In AR-positive bladder cancer UMUC3 and TCC-SUP cells, dihydrotestosterone (DHT) increased the expression of EGFR and ERBB2 both in mRNA and in protein levels. DHT additionally upregulated the levels of phosphorylation of EGFR (pEGFR) and its downstream proteins AKT (pAKT) and ERK1/2 (pERK), induced by EGF treatment, in AR-positive cells[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CV-1 cell NEHyVXlHfW6ldHnvckBie3OjeR?= MmjSRYdwdmm|dHnjJIFkfGm4aYT5JEhGSzVyKTDh[4FqdnO2IHj1cYFvKGGwZILv[4VvKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDWk0yKGOnbHysJGVEPTB;MD6wNFYh|ryP MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QDdzNUO0K|46QDdzNUO0QE9iRg>?
MDA-MB-453 cell NWrLbYtPTnWwY4Tpc44h[XO|YYm= M{HlWWRqe3CuYXPlcYVvfCCxZjDbN2heTEiWIH\yc40hcHWvYX6gZY5lem:pZX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKE2GQT3NRk01PTNiY3XscJMh[nlid3jvcIUh[2WubDDy[YNmeHSxcjDibY5lcW6pIHHzd4F6NCCNaU2wMlAxODJizszN NFXxTG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{CzOFEyPyd-MUewN|QyOTd:L3G+
HepG2 cell NXv2bYxFTnWwY4Tpc44h[XO|YYm= MYnBZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKGGwZILv[4VvKHKnY3XweI9zKGW6cILld5Nm\CCrbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[WO2aY\heIlwdixiSVO1NF0xNjBzOEKg{txO M3vJblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUS5PFY3Lz5zN{G0PVg3PjxxYU6=
MDA453 cell M1PDdGZ2dmO2aX;uJIF{e2G7 MVrEbZNxdGGlZX3lcpQhd2ZiW{PIYWRJXCCocn;tJIh2dWGwIFHSJIlvKE2GQUS1N{Bk\WyuczygT4k:OC5yMEOg{txO MnfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzOEGxOFEoRjF5MUixNVQyRC:jPh?=
mouse C2C12 cell NWLidoZmTnWwY4Tpc44h[XO|YYm= NXTWfFdCSWexbnnzeEBi[3Srdnn0fUBifCC{YYSgRXIhfHKjboPm[YN1\WRiaX6gcY92e2ViQ{LDNVIh[2WubIOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGVEPTB;MD6wNlgh|ryP NUfFbIVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexPFEyPDFpPkG3NVgyOTRzPD;hQi=>
monkey COS7 cell MVLGeY5kfGmxbjDhd5NigQ>? NWT3NIhwSmmwZHnu[{Bi\m[rbnn0fUB1dyCqdX3hckBidmS{b3flckBz\WOncITvdkBmgHC{ZYPz[YQhcW5ibX;ub4V6KEORU{egZ4VtdHNiYomge4hwdGViY3XscEBjcW6maX7nJIF{e2G7LDDLbV0xNjByMEKg{txO MkXMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2NEK5NVIoRjF6NESyPVEzRC:jPh?=
CHO cell NGDabnVHfW6ldHnvckBie3OjeR?= MX7B[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIGTHVlUh\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDifUBtfWOrZnXyZZNmKGG|c3H5MEBGSzVyPUSuOFkh|ryP MnHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|MEeyPVQoRjF6M{C3Nlk1RC:jPh?=
COS7 cell NXfOdpZiTnWwY4Tpc44h[XO|YYm= MmfqRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDXO|QySyCvdYThcpQh\XiycnXzd4VlKGmwIFPPV|ch[2WubIOgZZN{\XO|ZXSgZZMhdHWlaX\ldoF{\SCjY4Tpeol1gSCjZoTldkAzPCCqcoOgZpkhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0xNjBzMjFOwG0> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB7NEK3PUc,OjJyOUSyO|k9N2F-
COS7 cell MlHQSpVv[3Srb36gZZN{[Xl? MVrB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IGS4O|dCKG23dHHueEBmgHC{ZYPz[YQhcW5iQ1;TO{Bk\WyuczDhd5Nme3OnZDDhd{BtfWOrZnXyZZNmKGGldHn2bZR6KGGodHXyJFI1KGi{czDifUBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVAvODF3IN88US=> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB7NEK3PUc,OjJyOUSyO|k9N2F-
COS7 cell NVftcHc3TnWwY4Tpc44h[XO|YYm= MUPB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEV1N5IHPlcIx{KGG|c3Xzd4VlKGG|IHz1Z4ln\XKjc3WgZYN1cX[rdImgZYZ1\XJiMkSgbJJ{KGK7IILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;MD6wNFYzKM7:TR?= NXjhOGk5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwPVQzPzlpPkKyNFk1Ojd7PD;hQi=>
LNCaP-hr cell NGG3R5JHfW6ldHnvckBie3OjeR?= Ml3QN{Bl[Xm| Mnj4RYdwdmm|dDDhZ5Rqfmm2eTDheEBCdmS{b3flckBz\WOncITvdkBVQDd5QTDteZRidnRiaX6gbJVu[W5iTF7DZXAucHJiY3XscJMh[XO|ZYPz[YQh[XNicILvd5RifGVic4DlZ4lncWNiYX70bYdmdiC|ZXPy[ZRqd25ibXXhd5Vz\WRiYX\0[ZIhOyCmYYnzJIJ6KGWweont[U1qdW23bn;hd5NigSxiRVO1NF0xNjBzNTFOwG0> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF7OUS3O{c,OjNzOUm0O|c9N2F-
LNCaP-hr cell M37UbGZ2dmO2aX;uJIF{e2G7 MX:zJIRigXN? NHP3ZXFC\2:waYP0JIFkfGm4aYT5JIF1KHerbHSgeJlx\SCDbnTyc4dmdiC{ZXPldJRweiCrbjDoeY1idiCOTlPhVE1peiClZXzsd{Bie3Onc4Pl[EBieyCycn;zeIF1\SC|cHXjbYZq[yCjboTp[4VvKHOnY4LleIlwdiCvZXHzeZJm\CCjZoTldkA{KGSjeYOgZpkh\W68eX3lMYludXWwb3Hzd4F6NCCHQ{WwQVAvODB4MjFOwG0> NH3Gd|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G5PVQ4Pyd-MkOxPVk1Pzd:L3G+
HEK293 cell M2fyZmZ2dmO2aX;uJIF{e2G7 M4r4TmFod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OC5zODDuUS=> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTNyNU[4PEc,OjV|MEW2PFg9N2F-
COS MXrGeY5kfGmxbjDhd5NigQ>? NVvoSlZISmmwZHnu[{Bi\m[rbnn0fUBi\2GrboP0JGFv\HKxZ3XuJJJm[2WydH;yJIV5eHKnc4Pl[EBEV1NiY3XscJMtKEurIE2gNE4xODJizszNMi=> M2HXfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl6N{G1N|QoRjl6N{G1N|Q9N2F-
COS-1 NXXUSIlGTnWwY4Tpc44h[XO|YYm= NYO1N4lISmmwZHnu[{Bi\m[rbnn0fUBnd3JiaIXtZY4hSW6mcn;n[Y4hemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPPV{0yKGOnbHzzMEBMcSB;IECuNFA{KM7:TT6= Mli5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTl{NUeyOUc,QTl{NUeyOVww[T5?
CV-1 MXvGeY5kfGmxbjDhd5NigQ>? MYPB[49vcXO2IHHjeIl3cXS7IHHnZYlve3RiSIXtZY4hSW6mcn;n[Y4hemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPWMVEh[2WubIOsJGVEPTBiPTCwMlAxPSEQvF2u MorqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTl{NUeyOUc,QTl{NUeyOVww[T5?
COS NWrZVo5{TnWwY4Tpc44h[XO|YYm= M3LUc2lvKH[rdILvJIJqdmSrbnegZYZncW6rdImgZZQhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4KgeJJidnOoZXP0[YQhcW62bzDDU3Mh[2WubIOuMEBKSzVyIE2gNE4xODRizszNMi=> NWK1ToRMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCyN|A3OjhpPkGwNlMxPjJ6PD;hQi=>
CV-1 MXTGeY5kfGmxbjDhd5NigQ>? MX\JckB3cXS{bzDh[49vcXO2aXOgZYN1cX[rdImgZYdicW6|dDDoeY1idiCjbnTyc4dmdiC{ZXPldJRweiC3c3nu[{Bkd3S{YX7z[oVkfGmxbjDhd5NigSCrbjDDWk0yKGOnbHzzMkwhTUN3MDC9JFAvODB4IN88UU4> M4rnfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMkOwOlI5Lz5zMEKzNFYzQDxxYU6=
CV-1 NFfiSW1HfW6ldHnvckBie3OjeR?= NXzKRm9qSWexbnnzeEBi[3Srdnn0fUB1dyC2aHWgbJVu[W5iYX7kdo9o\W5icnXj[ZB1d3JiKHjBVkkhcW5iQ2[tNUBk\WyuczygSWM2OCB;IECuNFA3KM7:TT6= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODN2ME[yOEc,OTB|NEC2NlQ9N2F-
CV1 NXLLd2l5TnWwY4Tpc44h[XO|YYm= MYXB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHnuJGNXOSClZXzsd{Bu\WG|dYLl[EBieyC|dHnteYxifGmxbjDv[kBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZYN1cX[rdImgZpkh[2:2cnHud4Zm[3Srb36gZZN{[XluIFXDOVAhRSByLkCwOVEh|ryPLh?= NIXwWpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{CzOFEyPyd-MUewN|QyOTd:L3G+
CV1 NWrpeohrTnWwY4Tpc44h[XO|YYm= MkTxRolv\GmwZzDh[oZqdmm2eTD0c{BpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS1ZzIHPlcIx{NCCNaTC9JFAvODByMjFOwG0v M1jYeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MkW3PFM5Lz5zN{K1O|g{QDxxYU6=
CV1 MmniSpVv[3Srb36gZZN{[Xl? Mmj5RYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS1ZzIHPlcIx{NCCHQ{WwJF0hOC5yMEWxJO69VS5? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzJ3N{izPEc,OTd{NUe4N|g9N2F-
MDA-MB-453 NXnHTlF1TnWwY4Tpc44h[XO|YYm= NXPPUJhOTGm|cHzhZ4Vu\W62IH;mJHs{UF2GSGSg[pJwdSCqdX3hckBCWiCneIDy[ZN{\WRiaX6gUWRCNU2ELUS1N{Bk\WyuczygT4khRSByLkCwNFMh|ryPLh?= NV7ybmZlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0N|kyOTJpPkG3OFM6OTF{PD;hQi=>
CV1 MmLSSpVv[3Srb36gZZN{[Xl? MYXB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEXjFiY3XscJMh[nlidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYYPzZZktKEWFNUCgQUAxNjByNUeg{txONg>? M2S3ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEO5NVEzLz5zN{SzPVEyOjxxYU6=
CV1 MV7GeY5kfGmxbjDhd5NigQ>? NV\KbGlpSWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBidmS{b3flckBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ2[xJINmdGy|IHL5JJRz[W6|Y4LpdJRqd26jbDDhZ5RqfmG2aX;uJIF{e2G7LDDFR|UxKD1iMD6wNFU4KM7:TT6= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzdyM{mzPEc,OTd5MEO5N|g9N2F-
MDA-MB-453 M3jy[GZ2dmO2aX;uJIF{e2G7 M1Xw[GRqe3CuYXPlcYVvfCCxZjDbN2heTEiWIH\yc40hcHWvYX6gRY5lem:pZX6gdoVk\XC2b4KgbY4hcHWvYX6gUWRCNU2ELUS1N{Bk\WyuczygT4khRSByLkCwNFkh|ryPLh?= NFzjbW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOESwNFQ6QSd-MUi0NFA1QTl:L3G+
CV1 M4ixNWZ2dmO2aX;uJIF{e2G7 MXHB[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIHHu[JJw\2WwIILlZ4VxfG:{IHnuJGNXOSClZXzsd{BjgSC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCjc4PhfUwhTUN3MDC9JFAvODB3NzFOwG0v MnOzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2MEC0PVkoRjF6NECwOFk6RC:jPh?=
Saos2 M1;jRWZ2dmO2aX;uJIF{e2G7 M{LWc2FkfGm4aYT5JIF1KGGwZILv[4VvKHKnY3XweI9zKGmwIHj1cYFvKFOjb4OyJINmdGy|IHHzd4V{e2WmIHHzJGlNPiC{ZYDy[ZN{cW:wLDDJR|UxKD1iMD6wNFAxPSEQvF2u MnzQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2NEK5NVIoRjF6NESyPVEzRC:jPh?=
CV1 NF\VdItHfW6ldHnvckBie3OjeR?= NYLwO293SWexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBidmS{b3flckBz\WOncITvdkBi\nKrY3HuJIdz\WWwIH3vcotmgSCFVkGgZ4VtdHNiYomgZ491emGwc3\lZ5Rqd25iYYPzZZktKEWFNUCgQUAxNjByNUGg{txONg>? MnfYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2NEK5NVIoRjF6NESyPVEzRC:jPh?=
MDA-kb2 NFjxS3pHfW6ldHnvckBie3OjeR?= NF7XNFhC\2:waYP0JIFkfGm4aYT5JIF1KGGwZILv[4VvKHKnY3XweI9zKGmwIHj1cYFvKE2GQT3rZlIh[2WubIOgZZN{\XO|ZXSgZZMhe3SrbYXsZZRqd25ib3[gcJVkcW[ncnHz[UBi[3Srdnn0fUBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEOTVyIE2gNE4xODB3IN88UU4> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV7MkK0OUc,OTl3OUKyOFU9N2F-
MDA-kb2 NHLvTnBHfW6ldHnvckBie3OjeR?= MX;B[49vcXO2IHHjeIl3cXS7IHH0JIdtfWOxY3;yeIlkd2mmIILlZ4VxfG:{IHnuJIh2dWGwIF3ERU1s[jJiY3XscJMh[XO|ZYPz[YQh[XNic4TpcZVt[XSrb36gc4YhdHWlaX\ldoF{\SCjY4Tpeol1gSCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFMUWwJF0hOC5yMEC1JO69VS5? NITmS|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW5NlI1PSd-MUm1PVIzPDV:L3G+
MDA-MB-453 MUTGeY5kfGmxbjDhd5NigQ>? NUW5[pVMTGm|cHzhZ4Vu\W62IH;mJHs{UF2vZYTofYx1emmnbn;sc45mKG[{b32gZY5lem:pZX6gdoVk\XC2b4KgbY4hcHWvYX6gUWRCNU2ELUS1N{Bk\WyuczygTWM2OCB;IECuNFAyKM7:TT6= M1zCXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NkC2PFcxLz5zOU[wOlg4ODxxYU6=
MDA-MB-453 M2rCTmZ2dmO2aX;uJIF{e2G7 MmfoRYdwdmm|dDDhZ5Rqfmm2eTDheEBidmS{b3flckBz\WOncITvdkBqdiCqdX3hckBOTEFvTVKtOFU{KGOnbHzzJJRz[W6|ZnXjeIVlKHerdHigUW1VXi2OVVOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIF3NWHYuVFSUL4Dyc41wfGW{IHzpcotm\CCOVVOg[4Vv\SCkeTDseYNq\mW{YYPlJJRz[W6|YXP0bZZifGmxbjDhd5NigSxiRVO1NEA:KDBwMECyJO69VS5? NUXSUHJwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm2NFY5PzBpPkG5OlA3QDdyPD;hQi=>
NIH3T3 NWj1T5RlTnWwY4Tpc44h[XO|YYm= NXe0RZNjSWexbnnzeEBi[3Srdnn0fUBifCCjbnTyc4dmdiC{ZXPldJRweiCOQlSgWFg4P0FibYX0ZY51KGmwIH3veZNmKE6LSEPUN{Bk\WyuczD0doFve2mnboTsfUB1emGwc3\lZ5Rm\CC5aYToJIJmfGFvZ3HsZYN1d3OrZHHz[UBz\XCxcoTldkBo\W6nIHHzd4V{e2WmIHHzJINmdGy3bHHyJJRz[W6|Zn;ycYF1cW:wIHL5JHIuW0GWIHHzd4F6NCCHQ{WwJF0hOC5yMEGyOVkh|ryPLh?= NEjxfWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUi1OlkzOSd-MUm4OVY6OjF:L3G+
NIH3T3 MX3GeY5kfGmxbjDhd5NigQ>? MnvsRYdwdmm|dDDhZ5Rqfmm2eTDheEBidmS{b3flckBz\WOncITvdkBqdiCvb4Xz[UBPUUh|VEOgZ4VtdHNidILhcpNq\W62bImgeJJidnOoZXP0[YQhf2m2aDDi[ZRiNWejbHHjeI9{cWSjc3WgdoVxd3K2ZYKg[4Vv\SCjc4Pld5Nm\CCjczDj[YxtfWyjcjD0doFve2[xcn3heIlwdiCkeTDSMXNCXCCjc4PhfUwhTUN3MDC9JFAvODB|OUixJO69VS5? NVXDcHBZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4OVY6OjFpPkG5PFU3QTJzPD;hQi=>
COS7 MXrGeY5kfGmxbjDhd5NigQ>? MVLB[49vcXO2IHHjeIl3cXS7IHH0JIFv\HKxZ3XuJJJm[2WydH;yJIxq\2GwZDDibY5lcW6pIHTvcYFqdiCneIDy[ZN{\WRiaX6gZYZzcWOjbjDndoVmdiCvb37r[ZkhS0:VNzDj[YxteyClbz30doFve2[nY4Tl[EB4cXSqIFfhcFQuTEKGIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwMEKg{txONg>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh4M{C4N{c,OTl6NkOwPFM9N2F-
Freestyle293F NFTmOHNHfW6ldHnvckBie3OjeR?= NYW2NlNIOyCqcoO= NX7oVYZ1TGm|cHzhZ4Vu\W62IH;mJHsyPy2jbIDoZU1u\XSqeXytN2heNW2rYn;s[ZJwdmViZoLvcUBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hUEWNMkmzJIRmemm4ZXSgSpJm\VO2eXzlNlk{TiClZXzsd{Bi\nSncjCzJIhzeyxiSVO1NEA:KDBwMECyNUDPxE1w MkTsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyOUSyO|koRjJ{MEm0Nlc6RC:jPh?=
Freestyle293F NX\WXYwyTnWwY4Tpc44h[XO|YYm= NEHMUWw{KGi{cx?= NVTLZYQyTGm|cHzhZ4Vu\W62IH;mJHsyPy2jbIDoZU1u\XSqeXytN2heNW2rYn;s[ZJwdmViZoLvcUBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjDUPFc4SSCvdYThcpQh\XiycnXzd4VlKGmwIFjFT|I6OyCmZYLpeoVlKE[{ZXXTeJlt\TJ7M1[gZ4VtdHNiYX\0[ZIhOyCqcoOsJGlEPTBiPTCwMlAxOjZizszNMi=> NFKy[3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkC5OFI4QSd-MkKwPVQzPzl:L3G+
HeLa NYHhUnVITnWwY4Tpc44h[XO|YYm= M3mxN3Rz[W6|YXP0bZZifGmxbjDv[kBpfW2jbjDhcoRzd2enbjDy[YNmeHSxcjD0doFve2[nY4Tl[EBqdiCqdX3hckBJ\UyjIHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhSWO2aY\peJkhRSByLkCwPEDPxE1w NUO0U2dQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4PVc3OTFpPkKyPFk4PjFzPD;hQi=>
HeLa M1e4V2Z2dmO2aX;uJIF{e2G7 NVfZdIRWWGG{dHnhcEBi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJIFv\HKxZ3XuJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKZVzhJINmdGy|IHPvMYV5eHKnc4PpcochSVKHIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDiZZNm\CC2cnHud4FkfGm4YYTpc44h[XO|YYmsJGFkfGm4aYT5JF0hOC5yMEig{txONg>? Ml\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7NUe5OFcoRjJ{OUW3PVQ4RC:jPh?=
Freestyle293F NW\XNYlpTnWwY4Tpc44h[XO|YYm= MYHJcohq[mm2aX;uJI9nKHerbHSgeJlx\SCDbnTyc4dmdiC{ZXPldJRweiBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDGdoVme3S7bHWyPVNHKGOnbHzzMEBKSzVyIE2gNE4xODJzIN88UU4> M4LjTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MUm5OFc4Lz5{M{G5PVQ4PzxxYU6=
Freestyle293F M3ixOGZ2dmO2aX;uJIF{e2G7 M3PTPWlvcGmkaYTpc44hd2ZiQX7kdo9o\W5icnXj[ZB1d3JiVEi3O2EhdXW2YX70JEh2dmuwb4fuJI9zcWerbjmg[ZhxemW|c3XkJIlvKE[{ZXXzeJlt\TJ7M1[gZ4VtdHNuIFnDOVAhRSByLkCwNlYh|ryPLh?= NHLlOY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G5PVQ4Pyd-MkOxPVk1Pzd:L3G+
COS7 MXzGeY5kfGmxbjDhd5NigQ>? NEiyNGkzKGi{cx?= NW\wZllqTGm|cHzhZ4Vu\W62IH;mJHs{UF1iXUXhcJBp[S2GSGSg[pJwdSCvb4Xz[UBidmS{b3flckBz\WOncITvdkBmgHC{ZYPz[YQhcW5ibX;ub4V6KEORU{egZ4VtdHNiYX\0[ZIhOiCqcoOgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzDhcoFtgXOrczygTWM2OCB;IECuNFAyKM7:TT6= M2HkPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK3PFE3Lz5{M{WyO|gyPjxxYU6=
C2C12 NInMTolHfW6ldHnvckBie3OjeR?= MYi1JIRigXN? M{nNU2Fod26rc4SgZYN1cX[rdImgZZQh[W6mcn;n[Y4hemWlZYD0c5Ih\XiycnXzd4VlKGmwIH3veZNmKEN{Q{GyJINmdGy|IHHzd4V{e2WmIHHzJI9{fGWxYnzhd5Qh\GmoZnXy[Y51cWG2aX;uJIFnfGW{IEWg[IF6eyxiRVO1NEA:KDBwMECwNFUh|ryPLh?= MlnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MECyPVAoRjJ2OUCwNlkxRC:jPh?=
C2C12 M{fPT2Z2dmO2aX;uJIF{e2G7 M{DIW2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gZY5lem:pZX6gdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKG2xdYPlJGMzSzF{IHPlcIx{KGK7IHHu[JJw\2WwLYPw[YNq\mmlIILld5BwdnOnIHXs[Y1mdnRvZILpeoVvKGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFAxOTNizszNMi=> Ml\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkG5OlQoRjJ3MUKxPVY1RC:jPh?=
COS7 MkDySpVv[3Srb36gZZN{[Xl? NXqwdZB{OjRiaILz Ml;lRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDBVkBmgHC{ZYPz[YQhcW5iQX\ybYNidiCpcnXlckBud26tZYmgR29UPyClZXzsd{Bi\nSncjCyOEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5yMEOg{txONg>? NXG2cGpmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0OVQ5PDlpPkK4OFU1QDR7PD;hQi=>
Shionogi NF\TOWpHfW6ldHnvckBie3OjeR?= MnLjNVAheE1? MXnJcoR2[3Srb36gc4YheHKxbHnm[ZJifGmxbjDv[kBud3W|ZTDTbIlwdm:paTDj[YxteyCjdDCxNEBxVQ>? M3fGU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{GxPFU2Lz5zN{exNVg2PTxxYU6=
MCF7 MXHGeY5kfGmxbjDhd5NigQ>? NWTxT3VUOzBidV2= NW\TWW1lOjRiaILz MnzaRY51[WexbnnzeEBi[3Srdnn0fUBifCCDUjDpckBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDBVkBt\X[nbDDheEA{OCC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nzJJJmdGG2aY\lJJRwKGOxboTyc4w> MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjBzNEe1NUc,OjJyMUS3OVE9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cyclin D1 / Cyclin D2 / Cyclin D3 / Cyclin E2 / Cyclin A1 / AR; 

PubMed: 26372468     


HPr-1AR cells were treated with 10 nM DHT or vehicle control for 24–48 hours (h), and immunoblot analysis of cell lysates reveals that cyclin D1 and D2 protein expression decreased substantially with DHT treatment compared to vehicle.

p-CDK2 / CDK2 / CDK4 / CDK6 / CDKN1A / p-RB / RB ; 

PubMed: 26372468     


HPr-1AR cells were treated with 10 nM DHT or vehicle control for 24–48 hours (h). Immunoblot analysis of cell lysates reveals that CDK4 and CDK6 protein expression decreased and CDKN1A expression increased with DHT treatment compared to vehicle. CDK2 phosphorylation at threonine 160 (T160) decreased nearly 50% with androgen treatment at 24 and 48 hours, albeit not significantly, and total CDK2 protein expression remained unchanged. Relative to total RB expression, the levels of various phosphorylated forms of RB (S780 and S807/811) decreased over time with androgen treatment. 

p27 / p-p27 ; 

PubMed: 22139837     


CWR22Rv1 or CWR22R3 cells were stimulated with 10 nm DHT for 6 h, with the addition of MG132 during the last 2 h before harvesting. Proteins were then immunoblotted.

AR / PP1 ; 

PubMed: 26636645     


HeLa cells were transfected with Flag-PP1α and HA-AR and then incubated for overnight in androgen-depleted medium without or with androgen (10 nM of DHT) as indicated for blotting. 

26372468 22139837 26636645
Immunofluorescence
FOXO1 / p27 ; 

PubMed: 22139837     


CWR22R3 cells cultured in medium with CDS serum were treated without/with DHT (10 nm) for 4 h. The cells were immunostained for FOXO1 and p27 and examined with a confocal microscope. 

AKT(S473) / PKCα(S657) ; 

PubMed: 22139837     


CWR22R3 cells cultured in medium with CDS serum were treated without/with 10 nm DHT (−/+ DHT) for 4 h. The cells were immunostained for AKT or PKCα phosphorylation and examined with a confocal microscope. Bars: 20 μm

22139837
In vivo DHT-treated SOD1-G93A mice demonstrate ameliorated muscle atrophy and increased body weight, which is associated with stronger grip-strength. DHT treatment increases the expression of insulin-like growth factor-1 in muscle, which can exert myotrophic as well as neurotrophic effects through retrograde transport. DHT treatment attenuates neuromuscular junction denervation, and axonal and motoneuron loss. DHT-treated SOD1-G93A mice demonstrates improvement in motor behavior as assessed by rota-rod and gait analyses, and an increased lifespan[2].

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: Bladder cancer cells
  • Concentrations: 1 nM
  • Incubation Time: 24 h
  • Method:

    Bladder cancer cells at a density of 50-60% confluence in 24-well plates are co-transfected with 250 ng of MMTV-luc reporter plasmid DNA and 2.5 ng of PRL-TK-luc plasmid DNA. After 6 h of transfection, the medium is replaced with another medium supplemented with charcoal-stripped FBS in the presence or absence of ligands (DHT, HF, or both) for 24 h. Cells were harvested, lysed, and assayed for luciferase activity.


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: SOD1-G93A mutant mice with the congenic C57BL/6J background
  • Dosages: 5 mg
  • Administration: silastic implant
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 58 mg/mL (199.69 mM)
Ethanol 58 mg/mL (199.69 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 290.44
Formula

C19H30O2

CAS No. 521-18-6
Storage powder
in solvent
Synonyms androstanolone, stanolone, 5α-DHT
Smiles CC12CCC(=O)CC1CCC3C2CCC4(C3CCC4O)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04032067 Not yet recruiting Drug: GV1001 placebo|Drug: Proscar placebo Benign Prostatic Hyperplasia (BPH) GemVax & Kael August 28 2019 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Androgen Receptor Signaling Pathway Map

Related Androgen Receptor Products

Tags: buy Dihydrotestosterone (DHT) | Dihydrotestosterone (DHT) supplier | purchase Dihydrotestosterone (DHT) | Dihydrotestosterone (DHT) cost | Dihydrotestosterone (DHT) manufacturer | order Dihydrotestosterone (DHT) | Dihydrotestosterone (DHT) distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID