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Desmin - Cytoskeleton Marker Rabbit mAb

Catalog No.: F1188

Application: Reactivity: Mouse, Rat, Guinea pig, Human

Usage Information

Dilution
WB1:100000 IHC1:2000 IF1:100 - 1:1000 FCM1:70

Application
WB, IHC, IF, FCM

Reactivity
Mouse, Rat, Guinea pig, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
53 kDa 35-50 kDa,53 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Desmin-Cytoskeleton Marker Rabbit mAb detects endogenous levels of total Desmin-Cytoskeleton Marker protein.

Clone
C15C10

Synonym(s)
Desmin, DES

Background
Desmin is a muscle-specific type III intermediate filament protein belonging to the intermediate filament family, essential for maintaining the structural and mechanical integrity of muscle cells. It is composed of three main domains: a conserved α-helical rod domain that forms coiled-coil dimers, a variable non-helical N-terminal head domain important for filament assembly, and a carboxy-terminal tail domain responsible for filament integration and interactions with other proteins and organelles. Desmin filaments form a fibrous network that connects myofibrils at the Z-disks, linking them laterally and anchoring the contractile apparatus to the sarcolemma, nucleus, and mitochondria. This architecture facilitates force transmission during muscle contraction and maintains cellular resilience against mechanical stress. Desmin acts as a critical cytoskeletal marker and mechanosensor, coordinating the spatial organization of organelles and contributing to mitochondrial positioning and respiratory function. Desmin interacts with nuclear lamins at the nuclear envelope and plays a role in transcriptional regulation during cardiac muscle development, including the regulation of the NKX2-5 gene involved in cardiomyogenesis. Its nuclear localization signals and post-translational modifications regulate both its assembly and potential nuclear functions. Mutations in the DES gene cause desminopathies, leading to skeletal and cardiac myopathies characterized by disrupted muscle architecture, impaired mechanotransduction, and progressive muscle degeneration.

Background

Desmin is a muscle-specific type III intermediate filament protein belonging to the intermediate filament family, essential for maintaining the structural and mechanical integrity of muscle cells. It is composed of three main domains: a conserved α-helical rod domain that forms coiled-coil dimers, a variable non-helical N-terminal head domain important for filament assembly, and a carboxy-terminal tail domain responsible for filament integration and interactions with other proteins and organelles. Desmin filaments form a fibrous network that connects myofibrils at the Z-disks, linking them laterally and anchoring the contractile apparatus to the sarcolemma, nucleus, and mitochondria. This architecture facilitates force transmission during muscle contraction and maintains cellular resilience against mechanical stress. Desmin acts as a critical cytoskeletal marker and mechanosensor, coordinating the spatial organization of organelles and contributing to mitochondrial positioning and respiratory function. Desmin interacts with nuclear lamins at the nuclear envelope and plays a role in transcriptional regulation during cardiac muscle development, including the regulation of the NKX2-5 gene involved in cardiomyogenesis. Its nuclear localization signals and post-translational modifications regulate both its assembly and potential nuclear functions. Mutations in the DES gene cause desminopathies, leading to skeletal and cardiac myopathies characterized by disrupted muscle architecture, impaired mechanotransduction, and progressive muscle degeneration.

References
https://pubmed.ncbi.nlm.nih.gov/35068951/ https://pubmed.ncbi.nlm.nih.gov/23143191/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%