Compstatin

Catalog No.S8522

For research use only.

Compstatin binds to complement component C3 and inhibits complement activation with IC50 of 12 μM.

Compstatin Chemical Structure

CAS No. 206645-99-0

Selleck's Compstatin has been cited by 2 Publications

Purity & Quality Control

Choose Selective Complement System Inhibitors

Biological Activity

Description Compstatin binds to complement component C3 and inhibits complement activation with IC50 of 12 μM.
Targets
complement component C3 [4]
12 μM
In vitro

Compstatin binds to native C3 and inhibits its cleavage by C3 convertase. Binding of Compstatin to native C3 is a multistep reaction that is preferred over its binding to the C3 fragments C3b and C3c. N-acetylation of Compstatin provides stability against enzymatic degradation[1]. Compstatin features a high selectivity for human and primate C3. While binding to C3 from baboons and other primates shows comparable interaction profiles as its human counterpart, no binding is detected for C3 from lower mammalian species such as mice, rats, guinea pigs, rabbits, or pigs[3].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RPE cells MVXGeY5kfGmxbjDhd5NigQ>? MlXvOVAhfU1? MXzJcohq[mm2aX;uJI9nKGOxbYDs[Y1mdnRiYXP0bZZifGmxbjDpckBpfW2jbjDm[ZRidCCUUFWgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKEN3Yj25JIZwem2jdHnvckBifCB3MDD1UUBjgSCobIXvdoV{[2WwY3WgZZN{[XliaX6gdJJme2WwY3Wgc4Yh[2:vcHzlcYVvfC2lb33w[ZRidnRiaIXtZY4he2W{dX2= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR7NEC0NEc,OjV2OUSwOFA9N2F-
fetal RPE cells MlzxSpVv[3Srb36gZZN{[Xl? NEnTcZMyPSCvaX7z MV7Jcohq[mm2aX;uJI9nKGOxbYDs[Y1mdnRiYXP0bZZifGmxbjDpckBpfW2jbjDm[ZRidCCUUFWgZ4VtdHNiYYPz[ZN{\WRiYYOgbIVud2y7c3nzJI9nKHOncoXtMZRz\WG2ZXSgdoFj[mm2IHXyfZRpem:leYTld{Bkd22yb4Xu[EBxemWrbnP1ZoF1\WRid3n0bEB{\XK3bTDmc5IhOTVibXnud{Bu\WG|dYLl[EAzOCCvaX7zJIFnfGW{IHXyfZRpem:leYTlJIFl\Gm2aX;uJIJ6KGinbX;sfZRq[yCjc4PhfS=> MmTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2OUSwOFAoRjJ3NEm0NFQxRC:jPh?=
In vivo Compstatin completely inhibits in vivo heparin/protamine-induced complement activation without adverse effects on heart rate or systemic arterial, central venous, and pulmonary arterial pressures. Compstatin is a safe and effective complement inhibitor that has the potential to prevent complement activation during and after clinical cardiac surgery. Furthermore, Compstatin can serve as the prototype for designing an orally administrated drug. In the extracorporeal circulation model, Compstatin inhibits the generation of C3a and C5a, the formation of the membrane attack complex, and CD11b expression on neutrophils (PMNs). The peptide also inhibits binding of C3/C3 fragments, PMNs, and monocytes to the polymer surface[2].

Protocol (from reference)

Animal Research:

[2]

  • Animal Models: Juvenile baboons (P. anubis)
  • Dosages: 17, 21, 34, 38.5, 50, 63.5 mg/kg
  • Administration: by bolus injection, infusion, or both

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 1550.77
Formula

C66H99N23O17S2

CAS No. 206645-99-0
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC(C)C(C(=O)NC1CSSCC(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC1=O)C(C)C)C(C)C)CCC(=O)N)CC(=O)O)CC2=CNC3=CC=CC=C32)CC4=CN=CN4)CC5=CN=CN5)CCCN=C(N)N)C(=O)NC(C(C)O)C(=O)N)N

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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