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BACE1 Rabbit mAb

Catalog No.: F3242

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IP1:50 IHC1:50

Application
WB, IP, IHC

Source
Rabbit

Reactivity
Human, Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
56 kDa

Positive Control	Rat cerebrum tissue; Mouse hippocampus ; Mouse cerebrum tissue; Mouse liver tissue; Rat cerebrum tissue; Rat hippocampus; Rat brain; Mouse brain; SH-SY5Y cell; HAP1 cell; Neuro-2a cell
Negative Control	Mouse ovary lysate; Neuro-2a; C6 cell; SH-SY5Y cell

Datasheet & SDS

Biological Description

Specificity
BACE1 Rabbit mAb recognizes endogenous levels of total BACE1 protein.

Uniprot ID
P56817

Clone
L19L22

Synonym(s)
BACE; KIAA1149; BACE1; Beta-secretase 1; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2; ASP2; Asp 2; Beta-site APP cleaving enzyme 1

Background
BACE1 (β-site APP-cleaving enzyme 1), also known as β-secretase, is a membrane-associated aspartyl protease crucial for the initiation of amyloid-β (Aβ) peptide production, a hallmark of Alzheimer's disease (AD) pathology. It cleaves amyloid precursor protein (APP) at the β-site to generate a C-terminal fragment (C99), which is subsequently processed by γ-secretase to produce Aβ peptides, particularly the pathogenic Aβ42 variant. Structurally, BACE1 is a type I transmembrane protein with a luminal catalytic domain containing two conserved aspartate residues (Asp32 and Asp228) essential for its enzymatic activity, along with a transmembrane domain and a short cytoplasmic tail. It also contains characteristic motifs like the DTG and DSG sequences within its active site. BACE1 is predominantly expressed in neurons and localized in acidic compartments such as endosomes, where APP processing occurs. Beyond APP cleavage, BACE1 has physiological substrates including neuregulin-1, voltage-gated sodium channel β-subunits, and others involved in myelination, synaptic function, and axon guidance, indicating its broader role in neurodevelopment and function. Elevated BACE1 activity and expression are observed in AD brains, and its genetic deletion in animal models leads to reduced Aβ levels and amyloid plaque formation.

Background

BACE1 (β-site APP-cleaving enzyme 1), also known as β-secretase, is a membrane-associated aspartyl protease crucial for the initiation of amyloid-β (Aβ) peptide production, a hallmark of Alzheimer's disease (AD) pathology. It cleaves amyloid precursor protein (APP) at the β-site to generate a C-terminal fragment (C99), which is subsequently processed by γ-secretase to produce Aβ peptides, particularly the pathogenic Aβ42 variant. Structurally, BACE1 is a type I transmembrane protein with a luminal catalytic domain containing two conserved aspartate residues (Asp32 and Asp228) essential for its enzymatic activity, along with a transmembrane domain and a short cytoplasmic tail. It also contains characteristic motifs like the DTG and DSG sequences within its active site. BACE1 is predominantly expressed in neurons and localized in acidic compartments such as endosomes, where APP processing occurs. Beyond APP cleavage, BACE1 has physiological substrates including neuregulin-1, voltage-gated sodium channel β-subunits, and others involved in myelination, synaptic function, and axon guidance, indicating its broader role in neurodevelopment and function. Elevated BACE1 activity and expression are observed in AD brains, and its genetic deletion in animal models leads to reduced Aβ levels and amyloid plaque formation.

References
[1] Yan R. Front Mol Neurosci. 2017 Apr 19;10:97. [2] Cole SL, et al. Curr Alzheimer Res. 2008 Apr;5(2):100-20.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%