ATP-binding cassette sub-family A member 3 Mouse mAb

Catalog No.: F2545

Application: Reactivity: Mouse, Rat

Usage Information

Dilution
IHC1:10

Application
IHC

Source
Mouse

Reactivity
Mouse, Rat

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

Biological Description

Specificity
ATP-binding cassette sub-family A member 3 Mouse mAb detects endogenous levels of total ATP-binding cassette sub-family A member 3 protein.

Clone
F19C21

Synonym(s)
Phospholipid-transporting ATPase ABCA3, ATP-binding cassette sub-family A member 3, Xenobiotic-transporting ATPase ABCA3, Abca3

Background
ATP-binding cassette sub-family A member 3 (ABCA3) is a highly conserved, multi-pass transmembrane glycoprotein in the ABC transporter superfamily that uses ATP hydrolysis to transport phospholipids into lamellar bodies (LBs) of alveolar type II cells, where pulmonary surfactant is assembled and stored. Structurally, ABCA3 consists of two similar halves, each containing six transmembrane domains and a cytosolic nucleotide-binding domain (NBD) with Walker A, Walker B, and ABCA-specific signature motifs, plus two essential N-linked glycosylation sites at Asn124 and Asn140 required for proper folding, stability, and LB targeting. Although expressed in multiple tissues, ABCA3 is highly enriched in the lung, where it is critical for LB biogenesis and surfactant homeostasis, particularly in transporting phosphatidylcholine, phosphatidylglycerol, and cholesterol. Mutations in ABCA3 can cause trafficking or functional defects, leading to neonatal surfactant deficiency, interstitial lung disease, and fatal respiratory distress.

Background

ATP-binding cassette sub-family A member 3 (ABCA3) is a highly conserved, multi-pass transmembrane glycoprotein in the ABC transporter superfamily that uses ATP hydrolysis to transport phospholipids into lamellar bodies (LBs) of alveolar type II cells, where pulmonary surfactant is assembled and stored. Structurally, ABCA3 consists of two similar halves, each containing six transmembrane domains and a cytosolic nucleotide-binding domain (NBD) with Walker A, Walker B, and ABCA-specific signature motifs, plus two essential N-linked glycosylation sites at Asn124 and Asn140 required for proper folding, stability, and LB targeting. Although expressed in multiple tissues, ABCA3 is highly enriched in the lung, where it is critical for LB biogenesis and surfactant homeostasis, particularly in transporting phosphatidylcholine, phosphatidylglycerol, and cholesterol. Mutations in ABCA3 can cause trafficking or functional defects, leading to neonatal surfactant deficiency, interstitial lung disease, and fatal respiratory distress.

References
[1] Beers MF, et al. Cell Tissue Res. 2017 Mar;367(3):481-493.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%