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Anti-VGluT1 Rabbit Antibody [E6H10]

Catalog No.: F3458

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:2000 IF1:1000

Application
WB, IP, IHC, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
62 kDa 62 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human cerebral cortex; Human brain; Mouse cerebrum; Mouse brain; Rat brain; Rat cerebral cortex; Hippocampal rat neurons/glia
Negative Control	Human liver; Mouse liver; Rat liver

Datasheet & SDS

Biological Description

Specificity
VGluT1 Rabbit mAb detects endogenous levels of total VGLUT1 protein.

Uniprot ID
Q9P2U7

Clone
E6H10

Synonym(s)
BNPI, VGLUT1, SLC17A7, Vesicular glutamate transporter 1, VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7

Background
VGLUT1 (Vesicular glutamate transporter 1) is a membrane protein belonging to the SLC17 family that mediates the ATP-dependent uptake of glutamate into presynaptic synaptic vesicles, enabling excitatory neurotransmission. Structurally, VGLUT1 is an integral membrane glycoprotein with 12 predicted transmembrane domains, localized to glutamatergic synaptic vesicles. In mammals, VGLUT1 is strongly expressed in the neocortex, cerebellum, and hippocampus, with species-specific variations—rodents show little subcortical expression, whereas primates, including prosimian galagos, display broader expression in both cortical and selected subcortical visual areas such as the lateral geniculate nucleus, pulvinar complex, and superior colliculus. Functionally, VGLUT1 supports intercortical and corticothalamic projections, facilitates precise laminar organization within cortical and subcortical visual structures, and complements VGLUT2—which predominates in thalamocortical and subcortical pathways—thus playing a key role in maintaining circuit-specific glutamatergic signaling.

Background

VGLUT1 (Vesicular glutamate transporter 1) is a membrane protein belonging to the SLC17 family that mediates the ATP-dependent uptake of glutamate into presynaptic synaptic vesicles, enabling excitatory neurotransmission. Structurally, VGLUT1 is an integral membrane glycoprotein with 12 predicted transmembrane domains, localized to glutamatergic synaptic vesicles. In mammals, VGLUT1 is strongly expressed in the neocortex, cerebellum, and hippocampus, with species-specific variations—rodents show little subcortical expression, whereas primates, including prosimian galagos, display broader expression in both cortical and selected subcortical visual areas such as the lateral geniculate nucleus, pulvinar complex, and superior colliculus. Functionally, VGLUT1 supports intercortical and corticothalamic projections, facilitates precise laminar organization within cortical and subcortical visual structures, and complements VGLUT2—which predominates in thalamocortical and subcortical pathways—thus playing a key role in maintaining circuit-specific glutamatergic signaling.

References
https://pubmed.ncbi.nlm.nih.gov/22912561/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%