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Anti-Stra8 Rabbit Antibody [F15D8]

Catalog No.: F3746

Application: Reactivity: Rat, Mouse

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:2000 IF1:100

Application
WB, IP, IHC, IF

Reactivity
Rat, Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
37 kDa 55 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Mouse testis tissue; Rat testis tissue; Mouse spleen tissue; Rat spleen tissue; F9 (retinoic acid, 1 µM, 24 h)
Negative Control	Mouse spleen tissue; Rat spleen tissue; F9

Datasheet & SDS

Biological Description

Specificity
Stra8 Rabbit mAb detects endogenous levels of total Stra8 protein.

Clone
F15D8

Synonym(s)
Stimulated by retinoic acid gene 8 protein, Stra8

Background
Stra8 (Stimulated by Retinoic Acid gene 8) is a key transcription factor belonging to the family of genes activated by retinoic acid that plays a pivotal role in the initiation of meiosis during germ cell development in vertebrates. It is characterized by a helix-loop-helix (HLH)-like domain that facilitates DNA-binding and transcriptional regulatory functions, enabling it to act primarily as a transcriptional activator. Stra8 is activated downstream of retinoic acid signaling pathways, acting as a master regulator that triggers the mitosis-to-meiosis transition in both male and female germ cells by directly or indirectly promoting the expression of meiotic genes such as Rec8, Dmc1, and Spo11. It is crucial for pre-meiotic DNA replication and subsequent recombination events, facilitating chromosomal synapsis and homologous recombination critical to gametogenesis. Stra8 deficiency results in the failure to enter meiosis, leading to sterility and infertility in model organisms, underscoring its indispensable role. It also influences germ cell proliferation and survival, integrating meiotic cues with cell cycle control mechanisms. Aberrations in Stra8 expression or regulation are linked to reproductive disorders and impaired fertility.

Background

Stra8 (Stimulated by Retinoic Acid gene 8) is a key transcription factor belonging to the family of genes activated by retinoic acid that plays a pivotal role in the initiation of meiosis during germ cell development in vertebrates. It is characterized by a helix-loop-helix (HLH)-like domain that facilitates DNA-binding and transcriptional regulatory functions, enabling it to act primarily as a transcriptional activator. Stra8 is activated downstream of retinoic acid signaling pathways, acting as a master regulator that triggers the mitosis-to-meiosis transition in both male and female germ cells by directly or indirectly promoting the expression of meiotic genes such as Rec8, Dmc1, and Spo11. It is crucial for pre-meiotic DNA replication and subsequent recombination events, facilitating chromosomal synapsis and homologous recombination critical to gametogenesis. Stra8 deficiency results in the failure to enter meiosis, leading to sterility and infertility in model organisms, underscoring its indispensable role. It also influences germ cell proliferation and survival, integrating meiotic cues with cell cycle control mechanisms. Aberrations in Stra8 expression or regulation are linked to reproductive disorders and impaired fertility.

References
https://pubmed.ncbi.nlm.nih.gov/16461896/ https://pubmed.ncbi.nlm.nih.gov/26378784/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%