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Anti-S100A4 Rabbit Antibody [L7M10]

Catalog No.: F4023

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000-1:10000 IP1:10 - 1:100 IHC1:250 - 1:500 IF1:100 - 1:250 FCM1:80 - 1:800

Application
WB, IP, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
12 kDa 11 kDa, 12 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human tonsil; Human small intestine; Human lung; HeLa; A549; A-375; Jurkat
Negative Control

Datasheet & SDS

Biological Description

Specificity
S100A4 (L7M10) Rabbit mAb detects endogenous levels of total S100A4 protein.

Clone
L7M10

Synonym(s)
CAPL, MTS1, S100A4, Protein S100-A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1, S100 calcium-binding protein A4

Background
S100A4, also known as fibroblast-specific protein-1 (FSP1) or metastasin-1 (MTS1), is a highly conserved 101-amino-acid calcium-binding protein of the S100 family, characterized by two EF-hand motifs—an N-terminal pseudo-EF-hand and a C-terminal canonical EF-hand—that undergo calcium-dependent conformational changes enabling dimerization or oligomerization. Encoded on chromosome 1q21, S100A4 is widely expressed in diverse cell types, including fibroblasts, immune cells, endothelial cells, and neural cells, and is localized in the cytoplasm, nucleus, and extracellular space. Intracellularly, it regulates cytoskeletal organization, cell motility, invasion, apoptosis, and transcription factor activity, notably through interactions with proteins such as non-muscle myosin IIA, tropomyosin, and p53. Extracellularly, S100A4 functions as a cytokine-like damage-associated molecular pattern (DAMP), binding receptors such as RAGE, TLR4, and IL-10R to activate MAPK, NF-κB, and JAK/STAT pathways, thereby promoting inflammation, fibrosis, angiogenesis, and tissue remodeling. Its expression is associated with physiological roles in neurogenesis, cell differentiation, and immune regulation, as well as pathological processes including cancer metastasis, organ fibrosis, neurodegeneration, and chronic inflammatory diseases.

Background

S100A4, also known as fibroblast-specific protein-1 (FSP1) or metastasin-1 (MTS1), is a highly conserved 101-amino-acid calcium-binding protein of the S100 family, characterized by two EF-hand motifs—an N-terminal pseudo-EF-hand and a C-terminal canonical EF-hand—that undergo calcium-dependent conformational changes enabling dimerization or oligomerization. Encoded on chromosome 1q21, S100A4 is widely expressed in diverse cell types, including fibroblasts, immune cells, endothelial cells, and neural cells, and is localized in the cytoplasm, nucleus, and extracellular space. Intracellularly, it regulates cytoskeletal organization, cell motility, invasion, apoptosis, and transcription factor activity, notably through interactions with proteins such as non-muscle myosin IIA, tropomyosin, and p53. Extracellularly, S100A4 functions as a cytokine-like damage-associated molecular pattern (DAMP), binding receptors such as RAGE, TLR4, and IL-10R to activate MAPK, NF-κB, and JAK/STAT pathways, thereby promoting inflammation, fibrosis, angiogenesis, and tissue remodeling. Its expression is associated with physiological roles in neurogenesis, cell differentiation, and immune regulation, as well as pathological processes including cancer metastasis, organ fibrosis, neurodegeneration, and chronic inflammatory diseases.

References
https://pubmed.ncbi.nlm.nih.gov/33918416/ https://pubmed.ncbi.nlm.nih.gov/40078995/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%