Choose Your Country or Region

Anti-RIP Rabbit Antibody [N10M19]

Catalog No.: F4149

Application: Reactivity: Human, Monkey

Usage Information

Dilution
WB1:1000 IP1:200 IHC1:300 - 1:1200 IF1:800

Application
WB, IP, IHC, IF

Reactivity
Human, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
78 kDa

Positive Control	Human lung adenocarcinoma; Human ductal breast carcinoma; Human non-Hodgkin lymphoma; Human ductal gastric adenocarcinoma; Human colon carcinoma; Squamous cell lung carcinoma; Esophageal carcinoma; Human ovarian carcinoma; Human ovarian clear cell carcinoma; HT-29; PC-3; SK-OV-3; HeLa; HCC1 187; PANC-1; Jurkat; MCF7
Negative Control

Datasheet & SDS

Biological Description

Specificity
RIP (N10M19) Rabbit mA detects endogenous levels of full-length RIP protein.

Clone
N10M19

Synonym(s)
Receptor-interacting serine/threonine-protein kinase 1, Cell death protein RIP, Receptor-interacting protein 1, RIPK1, RIP 1, RIP1

Background
RIP (Receptor-interacting protein) kinases are a family of serine/threonine kinases defined by a conserved N-terminal kinase domain and variable C-terminal regulatory domains, such as the death domain (RIP1), caspase activation and recruitment domain (RIP2), RIP homotypic interaction motif (RIP1/3), ankyrin repeats (RIP4/5/6), leucine-rich repeats and Roc/COR domains (RIP6/7), and a WD40 motif (RIP7), which dictate their specific interactions and functions. These kinases are widely expressed and act as critical sensors of intracellular and extracellular stress signals, including cytokine stimulation, pathogen infection, DNA damage, and inflammation. Functionally, RIP kinases regulate diverse cellular processes, most prominently the NF-κB pathway, apoptosis, and necroptosis, thereby integrating immune responses, inflammation, and cell death decisions. Among them, RIP1 is central to death receptor signaling, RIP2 mediates innate immune responses, RIP3 is essential for necroptosis, and RIP6/7 have emerging roles in neurodegeneration such as Parkinson’s disease. Collectively, RIP kinases are key modulators of cell fate and immune regulation, with significant therapeutic potential in inflammatory, ischemic, and neurodegenerative disorders.

Background

RIP (Receptor-interacting protein) kinases are a family of serine/threonine kinases defined by a conserved N-terminal kinase domain and variable C-terminal regulatory domains, such as the death domain (RIP1), caspase activation and recruitment domain (RIP2), RIP homotypic interaction motif (RIP1/3), ankyrin repeats (RIP4/5/6), leucine-rich repeats and Roc/COR domains (RIP6/7), and a WD40 motif (RIP7), which dictate their specific interactions and functions. These kinases are widely expressed and act as critical sensors of intracellular and extracellular stress signals, including cytokine stimulation, pathogen infection, DNA damage, and inflammation. Functionally, RIP kinases regulate diverse cellular processes, most prominently the NF-κB pathway, apoptosis, and necroptosis, thereby integrating immune responses, inflammation, and cell death decisions. Among them, RIP1 is central to death receptor signaling, RIP2 mediates innate immune responses, RIP3 is essential for necroptosis, and RIP6/7 have emerging roles in neurodegeneration such as Parkinson’s disease. Collectively, RIP kinases are key modulators of cell fate and immune regulation, with significant therapeutic potential in inflammatory, ischemic, and neurodegenerative disorders.

References
https://pubmed.ncbi.nlm.nih.gov/20383176/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%