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Anti-Ret Rabbit Antibody [P19D19]

Catalog No.: F3758

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000 IP1:10 - 1:100 IHC1:50 IF1:50 - 1:100

Application
WB, IP, IHC, IF, ELISA

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
124 kDa 155 kDa,175 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Mouse colon tissue; Human thyroid gland carcinoma tissue; Human gastric carcinoma tissue; Human colon tissue; Mouse brain tissue; Rat brain tissue; Human Medullary thyroid carcinoma from GTCAC haplotype carriers; Neuro-2a; TT (human thyroid carcinoma); SH-SY5Y
Negative Control	Human Medullary thyroid carcinoma from GTCAC haplotype non-carriers

Datasheet & SDS

Biological Description

Specificity
Ret (P19D19) Rabbit mAb detects endogenous levels of total Ret protein.

Clone
P19D19

Synonym(s)
CDHF12, CDHR16, PTC, RET, RET51, Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12, Proto-oncogene c-Ret

Background
RET (Ret proto-oncogene) is a receptor tyrosine kinase belonging to the cadherin superfamily that transduces extracellular signals into intracellular responses critical for development and tissue homeostasis. Its extracellular domain contains four cadherin-like repeats and a cysteine-rich region, both of which are involved in calcium binding and essential for receptor activation. RET spans the membrane via a single transmembrane helix and has an intracellular tyrosine kinase domain with multiple conserved phosphorylation sites. Activation of RET occurs upon binding of GDNF family ligands (e.g., GDNF, neurturin) in complex with GFRα co-receptors, triggering receptor dimerization and autophosphorylation at specific tyrosines such as Tyr1062 and Tyr1015. These phosphorylated tyrosines serve as docking sites for adaptor proteins and initiate downstream signaling through the RAS/MAPK, PI3K/AKT, PLCγ/PKC, JAK/STAT, and Src kinase pathways. These cascades control key cellular processes such as proliferation, differentiation, survival, migration, and morphogenesis. RET signaling plays an essential role in the development of neural crest-derived tissues, including the enteric nervous system, peripheral neurons, adrenal medulla, and kidneys. Alternative splicing of the RET gene generates isoforms with different C-terminal tails that modulate the specificity and strength of downstream signaling. In adult tissues, RET maintains neurotrophic signaling and contributes to renal function and metabolic regulation, including body weight control via the GDF15-GFRAL-RET axis. Mutations or dysregulation of RET signaling are implicated in developmental disorders such as Hirschsprung disease and medullary thyroid carcinoma.

Background

RET (Ret proto-oncogene) is a receptor tyrosine kinase belonging to the cadherin superfamily that transduces extracellular signals into intracellular responses critical for development and tissue homeostasis. Its extracellular domain contains four cadherin-like repeats and a cysteine-rich region, both of which are involved in calcium binding and essential for receptor activation. RET spans the membrane via a single transmembrane helix and has an intracellular tyrosine kinase domain with multiple conserved phosphorylation sites. Activation of RET occurs upon binding of GDNF family ligands (e.g., GDNF, neurturin) in complex with GFRα co-receptors, triggering receptor dimerization and autophosphorylation at specific tyrosines such as Tyr1062 and Tyr1015. These phosphorylated tyrosines serve as docking sites for adaptor proteins and initiate downstream signaling through the RAS/MAPK, PI3K/AKT, PLCγ/PKC, JAK/STAT, and Src kinase pathways. These cascades control key cellular processes such as proliferation, differentiation, survival, migration, and morphogenesis. RET signaling plays an essential role in the development of neural crest-derived tissues, including the enteric nervous system, peripheral neurons, adrenal medulla, and kidneys. Alternative splicing of the RET gene generates isoforms with different C-terminal tails that modulate the specificity and strength of downstream signaling. In adult tissues, RET maintains neurotrophic signaling and contributes to renal function and metabolic regulation, including body weight control via the GDF15-GFRAL-RET axis. Mutations or dysregulation of RET signaling are implicated in developmental disorders such as Hirschsprung disease and medullary thyroid carcinoma.

References
https://pubmed.ncbi.nlm.nih.gov/16849421/ https://pubmed.ncbi.nlm.nih.gov/36947256/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%