Anti-Rab1A Rabbit Antibody [B9J11]

Catalog No.: F1424

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IF1:800

Application
WB, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
22 kDa

Positive Control	U-87MG; C6; C2C12; NIH/3T3
Negative Control

Datasheet & SDS

Biological Description

Specificity
Rab1A Rabbit mAb detects endogenous levels of total Rab1A protein.

Clone
B9J11

Synonym(s)
Ras-related protein Rab-1A, YPT1-related protein, RAB1A, RAB1.

Background
Rab1A is a highly conserved small guanosine triphosphatase (GTPase) that plays a central role in regulating intracellular vesicular transport from the endoplasmic reticulum (ER) to the Golgi apparatus. Beyond its trafficking function, Rab1A is also involved in signal transduction and the regulation of cell migration. Rab1A has been identified as an oncogene in several cancer types, including gastric cancer, nasopharyngeal carcinoma, breast cancer, lung cancer, and hepatocellular carcinoma. Interestingly, its expression is reduced in androgen-independent prostate cancer, and depletion of Rab1A has been shown to enhance the proliferative capacity of prostate cancer cells. Functionally, Rab1A binds to and recruits the COPII complex tethering factor p115 to a cis-SNARE complex on COPII-coated vesicles budding from the ER. It also interacts with Golgi tethering and docking proteins, such as giantin (GOLGB1) and golgin-84, to maintain Golgi architecture and function. Through these interactions, Rab1A facilitates the export of newly synthesized proteins from the ER to the Golgi. Like other Rab family members, Rab1A activity depends on intrinsic GTPase cycling, regulated by its associated guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).

Background

Rab1A is a highly conserved small guanosine triphosphatase (GTPase) that plays a central role in regulating intracellular vesicular transport from the endoplasmic reticulum (ER) to the Golgi apparatus. Beyond its trafficking function, Rab1A is also involved in signal transduction and the regulation of cell migration. Rab1A has been identified as an oncogene in several cancer types, including gastric cancer, nasopharyngeal carcinoma, breast cancer, lung cancer, and hepatocellular carcinoma. Interestingly, its expression is reduced in androgen-independent prostate cancer, and depletion of Rab1A has been shown to enhance the proliferative capacity of prostate cancer cells. Functionally, Rab1A binds to and recruits the COPII complex tethering factor p115 to a cis-SNARE complex on COPII-coated vesicles budding from the ER. It also interacts with Golgi tethering and docking proteins, such as giantin (GOLGB1) and golgin-84, to maintain Golgi architecture and function. Through these interactions, Rab1A facilitates the export of newly synthesized proteins from the ER to the Golgi. Like other Rab family members, Rab1A activity depends on intrinsic GTPase cycling, regulated by its associated guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).

References
https://pubmed.ncbi.nlm.nih.gov/34376787/ https://pubmed.ncbi.nlm.nih.gov/23555793/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%