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Anti-RAB10 Rabbit Antibody [K22D15]

Catalog No.: F3768

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 IP1:30 IF1:500 FCM1:600

Application
WB, IP, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
22 kDa 25 kDa, 22 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	A549; HCT 116; C6; PC-12; NIH/3T3; MCF7; HeLa
Negative Control

Datasheet & SDS

Biological Description

Specificity
Rab10 (K22D15) Rabbit mAb detects endogenous levels of total Rab10 protein.

Clone
K22D15

Synonym(s)
Ras-related protein Rab-10, RAB10

Background
Rab10 is a conserved small GTPase of the Rab subfamily within the Ras superfamily, functioning as a molecular switch that regulates intracellular membrane trafficking. It contains a highly conserved GTPase domain with essential motifs for guanine nucleotide binding and hydrolysis, including the phosphate-binding loop (P-loop), switch I, and switch II regions, which control its cycling between active (GTP-bound) and inactive (GDP-bound) states. These conformational changes guide Rab10’s interactions with guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), and specific effectors. Localized to the endoplasmic reticulum (ER), recycling endosomes, and Golgi apparatus, Rab10 promotes tubule elongation and maintenance of the ER network by recruiting effectors that link membranes to the microtubule cytoskeleton. It mediates basolateral cargo transport and regulates trafficking from the Golgi to the plasma membrane, and also participates in recycling endosome morphology and cargo sorting through effectors such as EHBP-1. Rab10 supports the polarized delivery of vesicles required for vital cellular processes, including axon growth and maintenance. Dysregulation of Rab10 disrupts membrane trafficking and leads to defects in cell polarity, organelle structure, and targeted cargo delivery.

Background

Rab10 is a conserved small GTPase of the Rab subfamily within the Ras superfamily, functioning as a molecular switch that regulates intracellular membrane trafficking. It contains a highly conserved GTPase domain with essential motifs for guanine nucleotide binding and hydrolysis, including the phosphate-binding loop (P-loop), switch I, and switch II regions, which control its cycling between active (GTP-bound) and inactive (GDP-bound) states. These conformational changes guide Rab10’s interactions with guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), and specific effectors. Localized to the endoplasmic reticulum (ER), recycling endosomes, and Golgi apparatus, Rab10 promotes tubule elongation and maintenance of the ER network by recruiting effectors that link membranes to the microtubule cytoskeleton. It mediates basolateral cargo transport and regulates trafficking from the Golgi to the plasma membrane, and also participates in recycling endosome morphology and cargo sorting through effectors such as EHBP-1. Rab10 supports the polarized delivery of vesicles required for vital cellular processes, including axon growth and maintenance. Dysregulation of Rab10 disrupts membrane trafficking and leads to defects in cell polarity, organelle structure, and targeted cargo delivery.

References
https://pubmed.ncbi.nlm.nih.gov/23263280/ https://pubmed.ncbi.nlm.nih.gov/20573983/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%