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Anti-PKCδ Rabbit Antibody [G9A5]

Catalog No.: F3769

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:5000 IHC1:2000 IF1:800 FCM1:250

Application
WB, IHC, IF, FCM

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
78 kDa 78 kDa, 80 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human fetal brain; Human fetal heart; Human transitional cell carcinoma of bladder; Human spleen; Mouse brain; Mouse thymus; Mouse liver; Rat thymus; Rat brain; Rat spleen; Rat testis; HEK-293T; HeLa; HAP1; Jurkat; C6; NIH/3T3; A431; U-87 MG
Negative Control

Datasheet & SDS

Biological Description

Specificity
PKCδ (G9A5) Rabbit mAb detects endogenous levels of total PKCδ protein.

Clone
G9A5

Synonym(s)
PKCD, PRKCD, Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta

Background
PKCδ (Protein kinase C δ), encoded by the PRKCD gene on chromosome 3, is a serine/threonine kinase of the novel PKC subfamily that plays a pivotal role in immune regulation, cell survival, proliferation, and apoptosis. Structurally, PKCδ consists of an N-terminal regulatory domain containing C1 and C2-like regions, a pseudosubstrate sequence, and a C-terminal catalytic domain with ATP- and substrate-binding sites, separated by a hinge (V3) region that undergoes phosphorylation and caspase-mediated cleavage during apoptosis. PKCδ is calcium-independent but activated by diacylglycerol (DAG), phorbol esters, serine/threonine phosphorylation (e.g., Thr505, Ser643, Ser662), and tyrosine phosphorylation by Src family kinases, with context-dependent lipid-dependent and lipid-independent activity. PKCδ is ubiquitously expressed, especially in lymphocytes, where it functions as an essential regulator of B-cell tolerance, it also functions as a critical regulator of B-cell development, immune tolerance, cell survival, proliferation, apoptosis, and stress responses, with PRKCD deficiency causing systemic lupus erythematosus (SLE)-like autoimmunity in humans and mice. Its dual role as both pro-survival and pro-apoptotic kinase underscores its importance in maintaining immune homeostasis and preventing autoimmunity.

Background

PKCδ (Protein kinase C δ), encoded by the PRKCD gene on chromosome 3, is a serine/threonine kinase of the novel PKC subfamily that plays a pivotal role in immune regulation, cell survival, proliferation, and apoptosis. Structurally, PKCδ consists of an N-terminal regulatory domain containing C1 and C2-like regions, a pseudosubstrate sequence, and a C-terminal catalytic domain with ATP- and substrate-binding sites, separated by a hinge (V3) region that undergoes phosphorylation and caspase-mediated cleavage during apoptosis. PKCδ is calcium-independent but activated by diacylglycerol (DAG), phorbol esters, serine/threonine phosphorylation (e.g., Thr505, Ser643, Ser662), and tyrosine phosphorylation by Src family kinases, with context-dependent lipid-dependent and lipid-independent activity. PKCδ is ubiquitously expressed, especially in lymphocytes, where it functions as an essential regulator of B-cell tolerance, it also functions as a critical regulator of B-cell development, immune tolerance, cell survival, proliferation, apoptosis, and stress responses, with PRKCD deficiency causing systemic lupus erythematosus (SLE)-like autoimmunity in humans and mice. Its dual role as both pro-survival and pro-apoptotic kinase underscores its importance in maintaining immune homeostasis and preventing autoimmunity.

References
https://pubmed.ncbi.nlm.nih.gov/27541826/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%