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Anti-Phospho-S6 Ribosomal Protein (S235/236) Rabbit Antibody [H18E15]

Catalog No.: F3530

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IF1:100 FCM1:200

Application
WB, IF, FCM

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
32 kDa

Positive Control	NIH/3T3 (PDGF, 100 ng/ml, 20 min); HeLa (20% serum-treated); Jurkat
Negative Control	NIH/3T3

Datasheet & SDS

Biological Description

Specificity
Phospho-S6 Ribosomal Protein (Ser235/236) (H18E15) Rabbit mAb detects endogenous levels of S6 Ribosomal Protein only when phosphorylated at Ser235/236.

Clone
H18E15

Synonym(s)
Small ribosomal subunit protein eS6; 40S ribosomal protein S6; Phosphoprotein NP33; RPS6; OK/SW-cl.2

Background
Phospho-S6 Ribosomal Protein (Ser235/236) is the phosphorylated form of ribosomal protein S6 (RPS6), a 249-amino acid (~28.6 kDa) protein and essential component of the 40S small ribosomal subunit within the S6E family. RPS6 plays a central role in regulating protein synthesis and cell growth, with phosphorylation occurring primarily at serine residues 235 and 236 in its carboxy-terminal region. These sites are among the earliest and most prominently modified in response to external stimuli and are phosphorylated mainly by p70/p85 S6 kinases (S6K1/2) downstream of the mTORC1 pathway, as well as by RSK, PKA, and PKC. Phosphorylation at Ser235/236 modulates ribosome function and fine-tunes translational control in response to growth factors, nutrients, and cellular stress, contributing to increased global translation, cell size regulation, and progression through the cell cycle. Phospho-S6 (Ser235/236) serves as a widely used biomarker of PI3K/AKT/mTOR pathway activation in diverse physiological and pathological contexts, including cancer proliferation, metabolic control, and neuronal function. Dysregulation of RPS6 phosphorylation or its upstream pathways is implicated in various diseases such as cancer, diabetes, and neurodegeneration.

Background

Phospho-S6 Ribosomal Protein (Ser235/236) is the phosphorylated form of ribosomal protein S6 (RPS6), a 249-amino acid (~28.6 kDa) protein and essential component of the 40S small ribosomal subunit within the S6E family. RPS6 plays a central role in regulating protein synthesis and cell growth, with phosphorylation occurring primarily at serine residues 235 and 236 in its carboxy-terminal region. These sites are among the earliest and most prominently modified in response to external stimuli and are phosphorylated mainly by p70/p85 S6 kinases (S6K1/2) downstream of the mTORC1 pathway, as well as by RSK, PKA, and PKC. Phosphorylation at Ser235/236 modulates ribosome function and fine-tunes translational control in response to growth factors, nutrients, and cellular stress, contributing to increased global translation, cell size regulation, and progression through the cell cycle. Phospho-S6 (Ser235/236) serves as a widely used biomarker of PI3K/AKT/mTOR pathway activation in diverse physiological and pathological contexts, including cancer proliferation, metabolic control, and neuronal function. Dysregulation of RPS6 phosphorylation or its upstream pathways is implicated in various diseases such as cancer, diabetes, and neurodegeneration.

References
https://pubmed.ncbi.nlm.nih.gov/18157089/ https://pubmed.ncbi.nlm.nih.gov/26733799/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%