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Anti-Phospho-RSK1 p90 (Ser380) Rabbit Antibody [G13M8]

Catalog No.: F2728

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:10000 IP1:20 IF1:50

Application
WB, IP, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
83 kDa 90 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	A431; C6; PC-12 (NGF, 200ng/ml, 24 h at 15 µg); HeLa (starved overnight, TPA, 200nM 4 h at 15 µg); NIH/3T3 (starved for 24 h, Calyculin A, 100nM, 30 minutes at 15 µg)
Negative Control	NIH/3T3

Datasheet & SDS

Biological Description

Specificity
Phospho-RSK1 p90 (Ser380) Rabbit mAb detects endogenous levels of RSK1 p90 only when phosphorylated at Ser380.

Clone
G13M8

Synonym(s)
MAPKAPK1A, RSK1, RPS6KA1, Ribosomal protein S6 kinase alpha-1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, MAP kinase-activated protein kinase 1a, Ribosomal S6 kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a, RSK-1

Background
Phospho-RSK1 p90 (Ser380) refers to the 90 kDa ribosomal S6 kinase 1 phosphorylated specifically at serine residue 380, a critical autophosphorylation site located in its C-terminal kinase (CTK) domain. RSK1 is a Ser/Thr protein kinase,a member of the p90 RSK family, composed of two distinct kinase domains—an N-terminal kinase (NTK) responsible for substrate phosphorylation and a CTK that regulates activation. Following ERK1/2-mediated phosphorylation of Thr573, the CTK becomes active and autophosphorylates Ser380, creating a docking site for phosphoinositide-dependent protein kinase 1 (PDK1). This event is essential for PDK1 to phosphorylate Ser221 in the NTK, fully activating RSK1. Ser380 phosphorylation thus serves as a pivotal molecular switch linking ERK signaling to downstream RSK1 activity, influencing key cellular processes such as proliferation, survival, and apoptosis. The phosphorylated Ser380 form is widely used as a biochemical marker for RSK1 activation status in studies of growth factor signaling, cancer biology, and stress responses.

Background

Phospho-RSK1 p90 (Ser380) refers to the 90 kDa ribosomal S6 kinase 1 phosphorylated specifically at serine residue 380, a critical autophosphorylation site located in its C-terminal kinase (CTK) domain. RSK1 is a Ser/Thr protein kinase,a member of the p90 RSK family, composed of two distinct kinase domains—an N-terminal kinase (NTK) responsible for substrate phosphorylation and a CTK that regulates activation. Following ERK1/2-mediated phosphorylation of Thr573, the CTK becomes active and autophosphorylates Ser380, creating a docking site for phosphoinositide-dependent protein kinase 1 (PDK1). This event is essential for PDK1 to phosphorylate Ser221 in the NTK, fully activating RSK1. Ser380 phosphorylation thus serves as a pivotal molecular switch linking ERK signaling to downstream RSK1 activity, influencing key cellular processes such as proliferation, survival, and apoptosis. The phosphorylated Ser380 form is widely used as a biochemical marker for RSK1 activation status in studies of growth factor signaling, cancer biology, and stress responses.

References
https://pubmed.ncbi.nlm.nih.gov/19808666/ https://pubmed.ncbi.nlm.nih.gov/20048145/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%