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Anti-Phospho-RNA Pol II CTD repeat YSPTSPS (S2) Rabbit Antibody [C9K19]

Catalog No.: F3793

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:5000 IP1:50 IHC1:100 IF1:100

Application
WB, IP, IHC, IF, FCM, ChIP

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
192 kDa 270 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Mouse kidney; Mouse spleen; Mouse testis; HeLa; PC-12; RAW 264.7; MCF7
Negative Control

Datasheet & SDS

Biological Description

Specificity
Phospho-RNA polymerase II CTD repeat YSPTSPS (Ser2) Rabbit mAb detects endogenous levels of RNA polymerase II only when the CTD repeat YSPTSPS only when phosphorylated at Ser2.

Clone
C9K19

Synonym(s)
RPB1, RPB220, SUA8, YDL140C, D2150, RPO21, DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit 1, RNA polymerase II subunit B1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B220

Background
Phospho‑RNA polymerase II CTD repeat YSPTSPS (Ser2) refers to the form of the C‑terminal domain (CTD) of RNA polymerase II’s largest subunit in which the serine residue at position 2 of the heptapeptide motif YSPTSPS is phosphorylated. The CTD contains multiple tandem repeats of this motif, and Ser2 is a major regulatory phosphorylation site. During the transcription cycle, Ser2 phosphorylation is primarily mediated by cyclin‑dependent kinases CDK9 (a component of the P‑TEFb complex) and CDK12, particularly during the elongation phase. This phosphorylation is typically low near transcription start sites and increases progressively toward the 3′ ends of genes. Ser2 phosphorylation is a molecular hallmark of RNA polymerase II engaged in productive elongation and is essential for recruiting RNA processing factors, including those involved in splicing, 3′ end cleavage, and polyadenylation. It helps coordinate transcription with chromatin modifications and mRNA export. The CTD itself is an intrinsically disordered, flexible domain that acts as a dynamic scaffold for regulatory proteins, with its phosphorylation pattern changing through the transcription cycle, Ser5 phosphorylation predominates during initiation, while Ser2 phosphorylation characterizes elongation. Although other residues, such as Ser5, Ser7, Tyr1, and Thr4, can also be phosphorylated, Ser2 is particularly critical for linking transcription elongation to RNA maturation. Dysregulation of Ser2 phosphorylation is implicated in cancers and neurodegenerative disorders.

Background

Phospho‑RNA polymerase II CTD repeat YSPTSPS (Ser2) refers to the form of the C‑terminal domain (CTD) of RNA polymerase II’s largest subunit in which the serine residue at position 2 of the heptapeptide motif YSPTSPS is phosphorylated. The CTD contains multiple tandem repeats of this motif, and Ser2 is a major regulatory phosphorylation site. During the transcription cycle, Ser2 phosphorylation is primarily mediated by cyclin‑dependent kinases CDK9 (a component of the P‑TEFb complex) and CDK12, particularly during the elongation phase. This phosphorylation is typically low near transcription start sites and increases progressively toward the 3′ ends of genes. Ser2 phosphorylation is a molecular hallmark of RNA polymerase II engaged in productive elongation and is essential for recruiting RNA processing factors, including those involved in splicing, 3′ end cleavage, and polyadenylation. It helps coordinate transcription with chromatin modifications and mRNA export. The CTD itself is an intrinsically disordered, flexible domain that acts as a dynamic scaffold for regulatory proteins, with its phosphorylation pattern changing through the transcription cycle, Ser5 phosphorylation predominates during initiation, while Ser2 phosphorylation characterizes elongation. Although other residues, such as Ser5, Ser7, Tyr1, and Thr4, can also be phosphorylated, Ser2 is particularly critical for linking transcription elongation to RNA maturation. Dysregulation of Ser2 phosphorylation is implicated in cancers and neurodegenerative disorders.

References
https://pubmed.ncbi.nlm.nih.gov/23028141/ https://pubmed.ncbi.nlm.nih.gov/18079404/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%