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Anti-Phospho-p53 (Ser9) Rabbit Antibody [G23F10]

Catalog No.: F3927

Application: Reactivity: Mouse

Usage Information

Dilution
WB1:500-1:2000

Application
WB, ELISA

Reactivity
Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Positive Control	NIH/3T3 (UV treated)
Negative Control	NIH/3T3

Datasheet & SDS

Biological Description

Specificity
Phospho-p53 (Ser9) Rabbit mAb detects endogenous levels of total p53 protein only when phosphorylated at serine 9.

Clone
G23F10

Synonym(s)
Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant p53; OTTMUSP00000006194; p53 cellular tumor antigen; Tumor suppressor p53; tumor supressor p53; Tumour Protein p53; bbl; bfy; bhy; p44; P53; Tp53; Trp53

Background
Phospho-p53 (Ser9) refers to the phosphorylated form of the tumor suppressor protein p53 at serine residue 9, located within the N-terminal transactivation domain. p53 is a key transcription factor that governs cell cycle arrest, apoptosis, DNA repair, and genomic stability in response to cellular stress and DNA damage. Ser9 phosphorylation is part of a broader network of post-translational modifications that modulate p53’s function. It typically occurs early upon genotoxic stress, mediated by kinases such as CK1δ/ε and ATM/ATR. This modification plays a crucial role in disrupting p53's interaction with its negative regulator MDM2, thereby preventing ubiquitination and degradation, and leading to p53 stabilization and activation. Phosphorylation at Ser9 may also induce conformational changes that enhance the recruitment of coactivators, enhancing the expression of p53 target genes involved in cell cycle checkpoints and programmed cell death. As part of a multisite modification code, Ser9 phosphorylation fine-tunes p53 activity depending on the type and intensity of cellular stress. Dysregulation of this phosphorylation event contributes to tumorigenesis.

Background

Phospho-p53 (Ser9) refers to the phosphorylated form of the tumor suppressor protein p53 at serine residue 9, located within the N-terminal transactivation domain. p53 is a key transcription factor that governs cell cycle arrest, apoptosis, DNA repair, and genomic stability in response to cellular stress and DNA damage. Ser9 phosphorylation is part of a broader network of post-translational modifications that modulate p53’s function. It typically occurs early upon genotoxic stress, mediated by kinases such as CK1δ/ε and ATM/ATR. This modification plays a crucial role in disrupting p53's interaction with its negative regulator MDM2, thereby preventing ubiquitination and degradation, and leading to p53 stabilization and activation. Phosphorylation at Ser9 may also induce conformational changes that enhance the recruitment of coactivators, enhancing the expression of p53 target genes involved in cell cycle checkpoints and programmed cell death. As part of a multisite modification code, Ser9 phosphorylation fine-tunes p53 activity depending on the type and intensity of cellular stress. Dysregulation of this phosphorylation event contributes to tumorigenesis.

References
https://pubmed.ncbi.nlm.nih.gov/22505655/ https://pubmed.ncbi.nlm.nih.gov/10930428/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%