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Anti-Phospho-HER2/ErbB2 (Tyr1196) Rabbit Antibody [L1F9]

Catalog No.: F0853

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:1000 IP1:100

Application
WB, IP

Reactivity
Human, Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
185 kDa

Positive Control	T47D (human neuregulin-1, 100 ng/ml, 5 min); MCF7 (human neuregulin-1, 100 ng/ml, 5 min)
Negative Control	T47D; MCF7

Datasheet & SDS

Biological Description

Specificity
Phospho-HER2/ErbB2 (Tyr1196) Rabbit mAb recognizes endogenous levels of HER2/ErbB2 protein only when phosphorylated at Tyr1196. The antibody may detect other activated ErbB family members.

Clone
L1F9

Synonym(s)
Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein (MLN 19), Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, ERBB2, HER2, MLN19, NEU, NGL

Background
The ErbB2 proto-oncogene, also known as HER2, encodes a 185 kDa transmembrane glycoprotein that functions as a receptor with intrinsic tyrosine kinase activity. Although ErbB2 does not have a known ligand, its kinase activity can be triggered independently of ligand binding, particularly when overexpressed or through dimerization with other members of the ErbB receptor family. ErbB2 gene amplification and protein overexpression occur in approximately 40% of human breast cancers, making it a critical therapeutic target for breast and other epithelial cancers. One potential approach to therapy involves enhancing the degradation of ErbB2 via a c-Cbl-regulated proteolytic pathway. Phosphorylation at tyrosine residue 877, located within the kinase domain and analogous to Tyr416 in pp60c-Src, may influence ErbB2's functional activity. Key autophosphorylation sites on ErbB2 include Tyr1248 and Tyr1221/1222, which facilitate signaling through the Ras-Raf-MAPK pathway. Additionally, autophosphorylation at Tyr1196 promotes interaction with the Crk adaptor protein, leading to Erk activation through a Ras-independent mechanism.

Background

The ErbB2 proto-oncogene, also known as HER2, encodes a 185 kDa transmembrane glycoprotein that functions as a receptor with intrinsic tyrosine kinase activity. Although ErbB2 does not have a known ligand, its kinase activity can be triggered independently of ligand binding, particularly when overexpressed or through dimerization with other members of the ErbB receptor family. ErbB2 gene amplification and protein overexpression occur in approximately 40% of human breast cancers, making it a critical therapeutic target for breast and other epithelial cancers. One potential approach to therapy involves enhancing the degradation of ErbB2 via a c-Cbl-regulated proteolytic pathway. Phosphorylation at tyrosine residue 877, located within the kinase domain and analogous to Tyr416 in pp60c-Src, may influence ErbB2's functional activity. Key autophosphorylation sites on ErbB2 include Tyr1248 and Tyr1221/1222, which facilitate signaling through the Ras-Raf-MAPK pathway. Additionally, autophosphorylation at Tyr1196 promotes interaction with the Crk adaptor protein, leading to Erk activation through a Ras-independent mechanism.

References
https://pubmed.ncbi.nlm.nih.gov/17274834/ https://pubmed.ncbi.nlm.nih.gov/11238891/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%