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Anti-PEX19 Rabbit Antibody [P24A3]

Catalog No.: F2617

Application: Reactivity: Human, Mouse

Usage Information

Dilution
WB1:1000 - 1:10000 IP1:10 - 1:100 IF1:50 - 1:100 FCM1:10 - 1:100

Application
WB, IP, IF, FCM

Reactivity
Human, Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
33 kDa 35 kDa,40 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Rat brain; Rat heart; Jurkat; MOLT-4; HeLa; A549
Negative Control

Datasheet & SDS

Biological Description

Specificity
PEX19 Rabbit mAb detects endogenous levels of total PEX19 protein.

Clone
P24A3

Synonym(s)
HK33, PXF, OK/SW-cl.22, PEX19, Peroxisomal biogenesis factor 19, 33 kDa housekeeping protein, Peroxin-19, Peroxisomal farnesylated protein.

Background
Peroxisomes are essential organelles found in all eukaryotic cells, playing a crucial role in maintaining cellular homeostasis. Peroxisomal biogenesis factor 19 (PEX19) is a central component in multiple stages of peroxisomal membrane protein (PMP) trafficking. Firstly, it functions as a chaperone in the cytosol, stabilizing newly synthesized PMPs. Secondly, PEX19 transports these proteins to the peroxisomal membrane, where it binds to the transmembrane protein PEX3, serving as a shuttling receptor. Thirdly, it facilitates the insertion of PMPs into the membrane. Additionally, the early transfer of PEX3 from the endoplasmic reticulum to the peroxisome depends on PEX19. In humans, PEX19 is a 299–amino acid protein with a flexible, intrinsically disordered N-terminal region that interacts with PEX3. The C-terminal domain (CTD) of PEX19 recognizes and binds cargo PMPs, a process essential for peroxisome biogenesis in vivo. Furthermore, farnesylation of PEX19 at its C-terminal CaaX motif strengthens its interaction with PMPs.

Background

Peroxisomes are essential organelles found in all eukaryotic cells, playing a crucial role in maintaining cellular homeostasis. Peroxisomal biogenesis factor 19 (PEX19) is a central component in multiple stages of peroxisomal membrane protein (PMP) trafficking. Firstly, it functions as a chaperone in the cytosol, stabilizing newly synthesized PMPs. Secondly, PEX19 transports these proteins to the peroxisomal membrane, where it binds to the transmembrane protein PEX3, serving as a shuttling receptor. Thirdly, it facilitates the insertion of PMPs into the membrane. Additionally, the early transfer of PEX3 from the endoplasmic reticulum to the peroxisome depends on PEX19. In humans, PEX19 is a 299–amino acid protein with a flexible, intrinsically disordered N-terminal region that interacts with PEX3. The C-terminal domain (CTD) of PEX19 recognizes and binds cargo PMPs, a process essential for peroxisome biogenesis in vivo. Furthermore, farnesylation of PEX19 at its C-terminal CaaX motif strengthens its interaction with PMPs.

References
https://pubmed.ncbi.nlm.nih.gov/28281558/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%