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Anti-OxPhos Complex IV subunit IV Mouse Antibody [P16M14]

Catalog No.: F3930

Application: Reactivity: Bovine, Human, Mouse, Rat, Zebrafish

Usage Information

Dilution
WB1:2000

Application
WB

Reactivity
Bovine, Human, Mouse, Rat, Zebrafish

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

Biological Description

Specificity
OxPhos Complex IV subunit IV Mouse mAb detects endogenous levels of total OxPhos Complex IV subunit IV protein.

Clone
P16M14

Synonym(s)
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX4I1; COX4

Background
OxPhos Complex IV subunit IV (COX4) is a critical nuclear-encoded subunit of Complex IV, also known as cytochrome c oxidase (COX)—the terminal enzyme in the mitochondrial electron transport chain. Complex IV catalyzes the transfer of electrons from cytochrome c to molecular oxygen, reducing it to water and simultaneously pumping protons across the inner mitochondrial membrane to establish the proton-motive force required for ATP synthesis. The complex comprises 14 subunits, including three mitochondrial DNA-encoded catalytic core subunits (COX1–3) that contain heme and copper centers essential for electron transfer, along with several nuclear-encoded accessory subunits, such as COX4, which contribute to complex stabilization and regulation. COX4 contains functional domains that facilitate interactions with other subunits and regulatory proteins, aiding in cytochrome c binding and enhancing proton pumping efficiency. COX4 also plays a key role in the assembly and biogenesis of Complex IV. It interacts with mitochondrial chaperones like mtHsp70 and Mge1, forming a chaperone-subunit complex that supports the efficient incorporation of COX4 into the enzyme complex and promotes the formation of respiratory supercomplexes, particularly under stress conditions such as heat shock. Loss or depletion of COX4 significantly disrupts Complex IV assembly and function, leading to reduced cytochrome c oxidase activity, impaired mitochondrial respiration, compromised membrane potential, and diminished ATP production.

Background

OxPhos Complex IV subunit IV (COX4) is a critical nuclear-encoded subunit of Complex IV, also known as cytochrome c oxidase (COX)—the terminal enzyme in the mitochondrial electron transport chain. Complex IV catalyzes the transfer of electrons from cytochrome c to molecular oxygen, reducing it to water and simultaneously pumping protons across the inner mitochondrial membrane to establish the proton-motive force required for ATP synthesis. The complex comprises 14 subunits, including three mitochondrial DNA-encoded catalytic core subunits (COX1–3) that contain heme and copper centers essential for electron transfer, along with several nuclear-encoded accessory subunits, such as COX4, which contribute to complex stabilization and regulation. COX4 contains functional domains that facilitate interactions with other subunits and regulatory proteins, aiding in cytochrome c binding and enhancing proton pumping efficiency. COX4 also plays a key role in the assembly and biogenesis of Complex IV. It interacts with mitochondrial chaperones like mtHsp70 and Mge1, forming a chaperone-subunit complex that supports the efficient incorporation of COX4 into the enzyme complex and promotes the formation of respiratory supercomplexes, particularly under stress conditions such as heat shock. Loss or depletion of COX4 significantly disrupts Complex IV assembly and function, leading to reduced cytochrome c oxidase activity, impaired mitochondrial respiration, compromised membrane potential, and diminished ATP production.

References
https://pubmed.ncbi.nlm.nih.gov/17091399/ https://pubmed.ncbi.nlm.nih.gov/23864706/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%