Anti-Olig1 Rabbit Antibody [K24N2]

Catalog No.: F3999

Application: Reactivity: Human, Rat

Usage Information

Dilution
WB1:1000-1:10000 FCM1:10 - 1:100

Application
WB, FCM

Reactivity
Human, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
27 kDa 35 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	MOLT4; SH-SY5Y; 293T; C6
Negative Control

Datasheet & SDS

Biological Description

Specificity
Olig1 Rabbit mAb detects endogenous levels of total Olig1 protein.

Clone
K24N2

Synonym(s)
BHLHB6, BHLHE21, OLIG1, Oligodendrocyte transcription factor 1, Oligo1, Class B basic helix-loop-helix protein 6, Class E basic helix-loop-helix protein 21, bHLHb6, bHLHe21

Background
Olig1 is a basic helix-loop-helix (bHLH) transcription factor encoded on human chromosome 21q22 that plays a critical role in oligodendrocyte lineage progression. Structurally, it contains a conserved bHLH domain that mediates dimerization and DNA binding at E-box motifs to regulate target gene expression. Olig1 is expressed in the developing and adult central nervous system, particularly in oligodendrocyte progenitor cells (OPCs), where it localizes to the nucleus but undergoes acetylation-dependent nuclear-to-cytoplasmic translocation as oligodendrocytes mature. Functionally, Olig1 promotes OPC differentiation, myelin gene expression, and remyelination after injury, with its nuclear presence required for remyelination and its cytoplasmic relocation linked to terminal maturation and morphological differentiation of oligodendrocytes.

Background

Olig1 is a basic helix-loop-helix (bHLH) transcription factor encoded on human chromosome 21q22 that plays a critical role in oligodendrocyte lineage progression. Structurally, it contains a conserved bHLH domain that mediates dimerization and DNA binding at E-box motifs to regulate target gene expression. Olig1 is expressed in the developing and adult central nervous system, particularly in oligodendrocyte progenitor cells (OPCs), where it localizes to the nucleus but undergoes acetylation-dependent nuclear-to-cytoplasmic translocation as oligodendrocytes mature. Functionally, Olig1 promotes OPC differentiation, myelin gene expression, and remyelination after injury, with its nuclear presence required for remyelination and its cytoplasmic relocation linked to terminal maturation and morphological differentiation of oligodendrocytes.

References
https://pubmed.ncbi.nlm.nih.gov/26631469/ https://pubmed.ncbi.nlm.nih.gov/33859549/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%