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Anti-Nucleolin Rabbit Antibody [K8A13]

Catalog No.: F3782

Application: Reactivity: Mouse, Rat, Human

Experiment Essentials

WB
Recommended WB dilution ratio: 1:10000

Usage Information

Dilution
WB1:10000 - 1:50000 IHC1:250 - 1:500 IF1:100 - 1:250 FCM1:2000

Application
WB, IHC, IF, FCM

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
76 kDa 100 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Fetal brain; Human colon; Mouse liver; Rat liver; HeLa; HT-29; K562; HuT-78
Negative Control

Datasheet & SDS

Biological Description

Specificity
Nucleolin (K8A13) Rabbit mAb detects endogenous levels of total Nucleolin protein.

Clone
K8A13

Synonym(s)
Nucleolin, Protein C23, NCL

Background
Nucleolin (NCL) is a multifunctional and dynamically localized protein that participates in a wide range of cellular processes. It plays a central role in the regulation of both DNA and RNA metabolism. As a histone chaperone and chromatin remodeler, it is involved in DNA replication, repair, and recombination. Nucleolin also contributes to ribosomal RNA transcription and processing, as well as ribosome assembly and nuclear export. Beyond this, it regulates mRNA transcription, splicing, stability, trafficking, and translation. In the cytoplasm, nucleolin assists in anchoring microtubules to centrosomes during interphase and promotes microtubule polymerization. At the plasma membrane, it modulates Ras protein assembly, protein–protein interactions, and MAPK signaling cascades. On the cell surface, nucleolin functions as a co-receptor for a variety of molecules, including cytokines, growth factors, and extracellular matrix proteins. Structurally, it contains two intrinsically disordered domains (IDDs), located at the N- and C-termini, which contribute to its versatility. Importantly, surface-expressed nucleolin acts as a receptor for several viruses, bacteria, and toxins. It has the unique ability to shuttle between the nucleus and cell surface, mediating bidirectional transport. This property allows nucleolin to facilitate the entry of pathogens into the nucleus, where they can exploit host cell functions to their advantage.

Background

Nucleolin (NCL) is a multifunctional and dynamically localized protein that participates in a wide range of cellular processes. It plays a central role in the regulation of both DNA and RNA metabolism. As a histone chaperone and chromatin remodeler, it is involved in DNA replication, repair, and recombination. Nucleolin also contributes to ribosomal RNA transcription and processing, as well as ribosome assembly and nuclear export. Beyond this, it regulates mRNA transcription, splicing, stability, trafficking, and translation. In the cytoplasm, nucleolin assists in anchoring microtubules to centrosomes during interphase and promotes microtubule polymerization. At the plasma membrane, it modulates Ras protein assembly, protein–protein interactions, and MAPK signaling cascades. On the cell surface, nucleolin functions as a co-receptor for a variety of molecules, including cytokines, growth factors, and extracellular matrix proteins. Structurally, it contains two intrinsically disordered domains (IDDs), located at the N- and C-termini, which contribute to its versatility. Importantly, surface-expressed nucleolin acts as a receptor for several viruses, bacteria, and toxins. It has the unique ability to shuttle between the nucleus and cell surface, mediating bidirectional transport. This property allows nucleolin to facilitate the entry of pathogens into the nucleus, where they can exploit host cell functions to their advantage.

References
https://pubmed.ncbi.nlm.nih.gov/35503380/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%