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Anti-nmt55/p54nrb Rabbit Antibody [C24P7]

Catalog No.: F3856

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:250 - 1:500 IF1:1500

Application
WB, IHC, IF

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
54 kDa 54 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Rat kidney tissue; Mouse liver tissue; Human prostate tissue; Human bladder carcinoma tissue; Human kidney tissue; HEK293T; MOLT-4; PC-12; RAW264.7; A673; PC3; MCF7; HeLa; NIH/3T3
Negative Control

Datasheet & SDS

Biological Description

Specificity
nmt55/p54nrb Rabbit mAb detects endogenous levels of total nmt55/p54nrb protein.

Clone
C24P7

Synonym(s)
NRB54, NONO, Non-POU domain-containing octamer-binding protein, NonO protein, 54 kDa nuclear RNA- and DNA-binding protein, 55 kDa nuclear protein, p54(nrb), p54nrb, NMT55

Background
NMT55/p54nrb, also known as NONO (Non-POU domain-containing octamer-binding protein), is a multifunctional nuclear DNA- and RNA-binding protein playing essential roles in transcription regulation, pre-mRNA splicing, RNA retention, DNA repair, and chromatin remodeling. As a member of the DBHS (Drosophila behavior/human splicing) protein family, NONO contains two RNA recognition motifs (RRMs) that facilitate interactions with RNA and protein partners, alongside coiled-coil domains enabling dimerization with related proteins SFPQ and PSPC1. It also possesses domains critical for DNA binding and nuclear localization. Acting as a core component of the spliceosome complex, NONO is vital for pre-mRNA processing, interacting with splicing factors such as PSF and topoisomerase I. Its activity is finely regulated through post-transcriptional modifications and the expression of different isoforms. Alterations or dysregulation of NONO results in some breast cancer subtypes. It also regulates transcription more broadly, influences circadian rhythm genes, and participates in innate immune responses by interacting with viral proteins and components of the cGAS-STING pathway. It stabilizes HIF-1/2 complexes, promoting the hypoxia response, angiogenesis, and tumor progression. Through its RNA-binding capabilities and intricate protein interactions, NONO coordinates complex gene expression networks essential for cell proliferation, differentiation, and stress responses. Mutations or dysfunction in NONO disrupt RNA metabolism and gene regulation, contributing to cancer.

Background

NMT55/p54nrb, also known as NONO (Non-POU domain-containing octamer-binding protein), is a multifunctional nuclear DNA- and RNA-binding protein playing essential roles in transcription regulation, pre-mRNA splicing, RNA retention, DNA repair, and chromatin remodeling. As a member of the DBHS (Drosophila behavior/human splicing) protein family, NONO contains two RNA recognition motifs (RRMs) that facilitate interactions with RNA and protein partners, alongside coiled-coil domains enabling dimerization with related proteins SFPQ and PSPC1. It also possesses domains critical for DNA binding and nuclear localization. Acting as a core component of the spliceosome complex, NONO is vital for pre-mRNA processing, interacting with splicing factors such as PSF and topoisomerase I. Its activity is finely regulated through post-transcriptional modifications and the expression of different isoforms. Alterations or dysregulation of NONO results in some breast cancer subtypes. It also regulates transcription more broadly, influences circadian rhythm genes, and participates in innate immune responses by interacting with viral proteins and components of the cGAS-STING pathway. It stabilizes HIF-1/2 complexes, promoting the hypoxia response, angiogenesis, and tumor progression. Through its RNA-binding capabilities and intricate protein interactions, NONO coordinates complex gene expression networks essential for cell proliferation, differentiation, and stress responses. Mutations or dysfunction in NONO disrupt RNA metabolism and gene regulation, contributing to cancer.

References
https://pubmed.ncbi.nlm.nih.gov/32168434/ https://pubmed.ncbi.nlm.nih.gov/11710964/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%