Anti-NFAT3 Rabbit Antibody [A4K24]

Catalog No.: F0789

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000 IP1:50

Application
WB, IP

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
120-140 kDa

Positive Control	A204
Negative Control

Datasheet & SDS

Biological Description

Specificity
NFAT3 Rabbit mAb detects endogenous levels of total NFAT3 protein.

Clone
A4K24

Synonym(s)
Nuclear factor of activated T-cells, cytoplasmic 4, NF-ATc4; NFATc4, T-cell transcription factor NFAT3, NFATC4, NFAT3

Background
The NFAT (Nuclear Factor of Activated T cells) protein family includes NFAT1 (also known as NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). These proteins function as transcription factors characterized by a Rel homology domain and work together with AP-1 (a Jun/Fos complex) to regulate gene expression critical for immune responses. While NFAT proteins are primarily found in immune cells, they are also present in other cell types such as skeletal muscle cells, keratinocytes, and adipocytes, where they play roles in controlling cell differentiation pathways. In their inactive state, NFAT proteins remain phosphorylated and sequestered in the cytoplasm. Upon an increase in intracellular calcium levels, the calcium/calmodulin-dependent phosphatase calcineurin is activated, leading to NFAT dephosphorylation and enabling their migration into the nucleus. NFAT signaling is downregulated when calcium levels drop, allowing kinases such as GSK-3 or CK1 to re-phosphorylate NFAT, prompting its return to the cytoplasm and cessation of transcriptional activity.

Background

The NFAT (Nuclear Factor of Activated T cells) protein family includes NFAT1 (also known as NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). These proteins function as transcription factors characterized by a Rel homology domain and work together with AP-1 (a Jun/Fos complex) to regulate gene expression critical for immune responses. While NFAT proteins are primarily found in immune cells, they are also present in other cell types such as skeletal muscle cells, keratinocytes, and adipocytes, where they play roles in controlling cell differentiation pathways. In their inactive state, NFAT proteins remain phosphorylated and sequestered in the cytoplasm. Upon an increase in intracellular calcium levels, the calcium/calmodulin-dependent phosphatase calcineurin is activated, leading to NFAT dephosphorylation and enabling their migration into the nucleus. NFAT signaling is downregulated when calcium levels drop, allowing kinases such as GSK-3 or CK1 to re-phosphorylate NFAT, prompting its return to the cytoplasm and cessation of transcriptional activity.

References
https://pubmed.ncbi.nlm.nih.gov/12975316/ https://pubmed.ncbi.nlm.nih.gov/11983154/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%