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Anti-KIFC1 Rabbit Antibody [F10F16]

Catalog No.: F2452

Application: Reactivity: Human

Usage Information

Dilution
WB1:10000 - 1:50000 IP1:10 - 1:100 IHC1:16000 IF1:50 - 1:100 FCM1:20

Application
WB, IP, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
74 kDa 74 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human stomach tissue; Human tonsil tissue; Human cervical carcinoma tissue; Jurkat; HepG2; 293T; HeLa; HAP1
Negative Control

Datasheet & SDS

Biological Description

Specificity
KIFC1 Rabbit mAb detects endogenous levels of total KIFC1 protein.

Clone
F10F16

Synonym(s)
HSET, KNSL2, KIFC1, Kinesin-like protein KIFC1, Kinesin-like protein 2, Kinesin-related protein HSET

Background
KIFC1 is a minus-end-directed motor protein from the kinesin-14 family, comprising three main domains: an N-terminal tail (residues 1–138), a central coiled-coil stalk (141–297), and a C-terminal motor domain (317–663) that contains ATPase and microtubule-binding activities essential for motor function. KIFC1 is vital for clustering supernumerary centrosomes into a bipolar mitotic spindle, preventing multipolar divisions and ensuring accurate chromosome segregation, processes critical in maintaining genomic stability. It also contributes to Golgi apparatus positioning by anchoring Golgi membranes near centrosomes through microtubule interaction. During the S phase of the cell cycle, KIFC1 transiently localizes to the nucleus, where it plays roles in DNA synthesis and chromatin maintenance. The protein regulates progression through the cell cycle by modulating the expression of cyclins A, B, and D, and interacts with spindle assembly checkpoint components to control mitotic timing. Overexpression of KIFC1 is frequently observed in cancers like lung, breast, ovarian, and liver cancers, where it promotes tumorigenesis by enhancing proliferation, stabilizing anti-apoptotic surviving protein, and facilitating invasion and metastasis, often via epithelial-mesenchymal transition (EMT). KIFC1 generates microtubule-associated forces that oppose kinesin-5 to cluster centrosomes and works cooperatively with NuMA to organize spindle poles and chromosome alignment.

Background

KIFC1 is a minus-end-directed motor protein from the kinesin-14 family, comprising three main domains: an N-terminal tail (residues 1–138), a central coiled-coil stalk (141–297), and a C-terminal motor domain (317–663) that contains ATPase and microtubule-binding activities essential for motor function. KIFC1 is vital for clustering supernumerary centrosomes into a bipolar mitotic spindle, preventing multipolar divisions and ensuring accurate chromosome segregation, processes critical in maintaining genomic stability. It also contributes to Golgi apparatus positioning by anchoring Golgi membranes near centrosomes through microtubule interaction. During the S phase of the cell cycle, KIFC1 transiently localizes to the nucleus, where it plays roles in DNA synthesis and chromatin maintenance. The protein regulates progression through the cell cycle by modulating the expression of cyclins A, B, and D, and interacts with spindle assembly checkpoint components to control mitotic timing. Overexpression of KIFC1 is frequently observed in cancers like lung, breast, ovarian, and liver cancers, where it promotes tumorigenesis by enhancing proliferation, stabilizing anti-apoptotic surviving protein, and facilitating invasion and metastasis, often via epithelial-mesenchymal transition (EMT). KIFC1 generates microtubule-associated forces that oppose kinesin-5 to cluster centrosomes and works cooperatively with NuMA to organize spindle poles and chromosome alignment.

References
https://pubmed.ncbi.nlm.nih.gov/31127080/ https://pubmed.ncbi.nlm.nih.gov/35111813/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%