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Anti-KIF1A Rabbit Antibody [F10B19]

Catalog No.: F3710

Application: Reactivity: Rat, Human

Usage Information

Dilution
WB1:5000 - 1:10000 IP1:50 IF1:100 FCM1:100

Application
WB, IP, IF, FCM

Reactivity
Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
191 kDa 191 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Rat hypothalamus lysate; Rat brain lysate; Human fetal brain lysate; HEK-293; 293; SH-SY5Y; U-87 MG
Negative Control

Datasheet & SDS

Biological Description

Specificity
KIF1A Rabbit mAb detects endogenous levels of total KIF1A protein.

Clone
F10B19

Synonym(s)
ATSV, C2orf20, KIF1A, Kinesin-like protein KIF1A, Axonal transporter of synaptic vesicles, Microtubule-based motor KIF1A, Unc-104- and KIF1A-related protein, hUnc-104

Background
KIF1A is a neuron-specific kinesin-3 family motor protein essential for anterograde transport of membranous cargo along microtubules in axons and dendrites. It facilitates the movement of synaptic vesicle precursors (SVPs), dense core vesicles (DCVs), and other cargoes toward microtubule plus ends. Structurally, KIF1A consists of an N-terminal motor domain with a catalytic core and neck linker that binds microtubules and hydrolyzes ATP to drive motility. A key feature of KIF1A is its K-loop (loop 12), a lysine-rich region enhancing binding to the polyglutamylated C-terminal tails of β-tubulin, thereby promoting strong microtubule attachment and high processivity. The neck coil supports dimerization and coordinated movement, while the tail domain contains coiled-coil motifs, a forkhead-associated (FHA) domain for phosphoprotein interactions, and a pleckstrin homology (PH) domain for cargo vesicle binding via lipid interactions. KIF1A transports critical neuronal components such as synaptophysin, synaptotagmin, Rab3A, and TrkA receptors, ensuring proper synaptic function and neuronal signaling. Mutations in KIF1A disrupt cargo transport, leading to KIF1A-associated neurological disorder (KAND), a group of progressive conditions marked by spasticity, intellectual disability, peripheral neuropathy, and brain abnormalities due to impaired axonal trafficking and synaptic dysfunction.

Background

KIF1A is a neuron-specific kinesin-3 family motor protein essential for anterograde transport of membranous cargo along microtubules in axons and dendrites. It facilitates the movement of synaptic vesicle precursors (SVPs), dense core vesicles (DCVs), and other cargoes toward microtubule plus ends. Structurally, KIF1A consists of an N-terminal motor domain with a catalytic core and neck linker that binds microtubules and hydrolyzes ATP to drive motility. A key feature of KIF1A is its K-loop (loop 12), a lysine-rich region enhancing binding to the polyglutamylated C-terminal tails of β-tubulin, thereby promoting strong microtubule attachment and high processivity. The neck coil supports dimerization and coordinated movement, while the tail domain contains coiled-coil motifs, a forkhead-associated (FHA) domain for phosphoprotein interactions, and a pleckstrin homology (PH) domain for cargo vesicle binding via lipid interactions. KIF1A transports critical neuronal components such as synaptophysin, synaptotagmin, Rab3A, and TrkA receptors, ensuring proper synaptic function and neuronal signaling. Mutations in KIF1A disrupt cargo transport, leading to KIF1A-associated neurological disorder (KAND), a group of progressive conditions marked by spasticity, intellectual disability, peripheral neuropathy, and brain abnormalities due to impaired axonal trafficking and synaptic dysfunction.

References
https://pubmed.ncbi.nlm.nih.gov/34487232/ https://pubmed.ncbi.nlm.nih.gov/36655764/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%