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Anti-KAP1 Rabbit Antibody [L21A12]

Catalog No.: F4064

Application: Reactivity: Human

Usage Information

Dilution
WB1:10000-1:50000 IP1:30 IHC1:100 - 1:250 IF1:100 - 1:250 FCM1:100

Application
WB, IP, IHC, IF, FCM

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
89 kDa 110 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human colon; Human kidney; HAP1 (treated with Blaomycin); HeLa; A431; MCF7
Negative Control

Datasheet & SDS

Biological Description

Specificity
KAP1 Rabbit mAb detects endogenous levels of total KAP1 protein.

Clone
L21A12

Synonym(s)
KAP1, RNF96, TIF1B, TRIM28, Transcription intermediary factor 1-beta, TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-associated protein 1, KRAB-interacting protein 1, Nuclear corepressor KAP-1, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, Tripartite motif-containing protein 28, KAP-1, KRIP-1

Background
KAP1(KRAB-associated protein 1), also known as TRIM28 or TIF1β, is a multifunctional transcriptional regulator of the TRIM protein family that plays essential roles in development, genome stability, and disease. Structurally, KAP1 contains an N-terminal RBCC domain (with a RING E3 ubiquitin ligase, two B-boxes, and a coiled-coil for dimerization and KRAB factor binding), a central intrinsically disordered region with an HP1-binding PxVxL motif, and a C-terminal PHD-BD cassette with SUMO E3 ligase and chromatin-binding activities. It is ubiquitously expressed in all human tissues, and its knockout causes embryonic lethality, underscoring its critical role in stem cell maintenance and early development. Functionally, KAP1 acts as a scaffold to recruit chromatin-modifying complexes such as NuRD (HDACs), SETDB1 (H3K9 methyltransferase), and HP1, enforcing transcriptional repression of targets such as endogenous retroviruses. However, phosphorylation- and SUMO-dependent regulation enables KAP1 to act not only as a repressor but also as an activator, with important implications in transcriptional pausing, DNA repair, immune regulation, viral gene expression, and cancer progression.

Background

KAP1(KRAB-associated protein 1), also known as TRIM28 or TIF1β, is a multifunctional transcriptional regulator of the TRIM protein family that plays essential roles in development, genome stability, and disease. Structurally, KAP1 contains an N-terminal RBCC domain (with a RING E3 ubiquitin ligase, two B-boxes, and a coiled-coil for dimerization and KRAB factor binding), a central intrinsically disordered region with an HP1-binding PxVxL motif, and a C-terminal PHD-BD cassette with SUMO E3 ligase and chromatin-binding activities. It is ubiquitously expressed in all human tissues, and its knockout causes embryonic lethality, underscoring its critical role in stem cell maintenance and early development. Functionally, KAP1 acts as a scaffold to recruit chromatin-modifying complexes such as NuRD (HDACs), SETDB1 (H3K9 methyltransferase), and HP1, enforcing transcriptional repression of targets such as endogenous retroviruses. However, phosphorylation- and SUMO-dependent regulation enables KAP1 to act not only as a repressor but also as an activator, with important implications in transcriptional pausing, DNA repair, immune regulation, viral gene expression, and cancer progression.

References
https://pubmed.ncbi.nlm.nih.gov/35281453/ https://pubmed.ncbi.nlm.nih.gov/26293668/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%