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Anti-JAB1 Mouse Antibody [A8M18]

Catalog No.: F3136

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:200-1:5000 IP1:50-1:500 IHC1:50-1:500 IF1:50-1:500

Application
WB, IP, IHC, IF

Reactivity
Mouse, Rat, Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
38 kDa

Positive Control	Human oral mucosa; Human colon; Rat liver; Mouse embryo; Cates-1b; Caki-1; Jurkat; NIH/3T3; PC-12; HeLa
Negative Control

Datasheet & SDS

Biological Description

Specificity
JAB1 Mouse mAb detects endogenous levels of total JAB1 protein.

Clone
A8M18

Synonym(s)
COP9 signalosome complex subunit 5, SGN5; Signalosome subunit 5, Jun activation domain-binding protein 1, COPS5, CSN5, JAB1

Background
Jab1/CSN5 was first identified as a coactivator of c-Jun and later recognized as the fifth subunit of the constitutive photomorphogenic-9 (COP9) signalosome (CSN), a multifunctional complex that regulates signal transduction, transcription, and protein stability. Also known as CSN5, this protein contains an Mpr1-Pad1 N-terminal (MPN) domain with a JAMM (JAB1/MPN/Mov34 metalloenzyme) motif, which is essential for the isopeptidase activity of the CSN. Through this function, CSN5 mediates deneddylation of cullin-RING ubiquitin ligases (CRLs), thereby controlling ubiquitin-dependent protein turnover. Functionally, Jab1/CSN5 promotes cell proliferation by inactivating various tumor suppressors and negative regulatory proteins through subcellular localization changes, degradation, or deneddylation. These targets include p53, Smad4/7, and the cyclin-dependent kinase inhibitor p27^Kip1. In addition, Jab1/CSN5 can stabilize specific proteins such as HIF-1α and c-Jun, and it also acts as a transcriptional cofactor for MYC, facilitating the expression of genes driving proliferation, angiogenesis, and invasion. Overexpression of Jab1/CSN5 has been reported across multiple cancer types, where it contributes to tumor initiation and progression. Its evolutionary conservation in humans, mice, fission yeast, and plants underscores its fundamental role in cell survival and growth regulation.

Background

Jab1/CSN5 was first identified as a coactivator of c-Jun and later recognized as the fifth subunit of the constitutive photomorphogenic-9 (COP9) signalosome (CSN), a multifunctional complex that regulates signal transduction, transcription, and protein stability. Also known as CSN5, this protein contains an Mpr1-Pad1 N-terminal (MPN) domain with a JAMM (JAB1/MPN/Mov34 metalloenzyme) motif, which is essential for the isopeptidase activity of the CSN. Through this function, CSN5 mediates deneddylation of cullin-RING ubiquitin ligases (CRLs), thereby controlling ubiquitin-dependent protein turnover. Functionally, Jab1/CSN5 promotes cell proliferation by inactivating various tumor suppressors and negative regulatory proteins through subcellular localization changes, degradation, or deneddylation. These targets include p53, Smad4/7, and the cyclin-dependent kinase inhibitor p27^Kip1. In addition, Jab1/CSN5 can stabilize specific proteins such as HIF-1α and c-Jun, and it also acts as a transcriptional cofactor for MYC, facilitating the expression of genes driving proliferation, angiogenesis, and invasion. Overexpression of Jab1/CSN5 has been reported across multiple cancer types, where it contributes to tumor initiation and progression. Its evolutionary conservation in humans, mice, fission yeast, and plants underscores its fundamental role in cell survival and growth regulation.

References
https://pubmed.ncbi.nlm.nih.gov/20955608/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%