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Anti-DAZL Rabbit Antibody [D5D23]

Catalog No.: F3719

Application: Reactivity: Human, Mouse, Rat

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:8000 IF1:1000

Application
WB, IP, IHC, IF

Reactivity
Human, Mouse, Rat

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
33 kDa 37 kDa, 38 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Mouse testis; Rat testis; Human testis
Negative Control

Datasheet & SDS

Biological Description

Specificity
DAZL Rabbit mAb detects endogenous levels of total DAZL protein.

Clone
D5D23

Synonym(s)
Dazl1, Dazla, Dazl, Deleted in azoospermia-like, DAZ-like autosomal, Deleted in azoospermia-like 1

Background
DAZL (Deleted in Azoospermia-Like) is a highly conserved RNA-binding protein that belongs to the DAZ gene family and is essential for germ cell development and gametogenesis in both males and females. It contains two key domains: an RNA Recognition Motif (RRM), approximately 90 amino acids in length, which specifically binds GUU- and UGUU(U/A)-rich sequences in the 3′ untranslated regions (3′UTRs) of target mRNAs, and a DAZ repeat domain, about 24 amino acids long, enriched in asparagine, tyrosine, and glutamine residues. This repeat domain facilitates protein–protein interactions, particularly with other RNA-binding proteins and components of the translation machinery. Through these domains, DAZL forms ribonucleoprotein complexes involved in post-transcriptional regulation. It controls the translation of thousands of mRNAs that govern critical processes in germ cells, including proliferation, meiotic entry, chromatin remodeling, spindle assembly, and synaptonemal complex formation. Depending on the cellular context, DAZL can act as a translational activator or repressor. A major function of DAZL is to recruit poly(A)-binding proteins (PABPs) and translation initiation factors to promote translation of target mRNAs, even those with short poly(A) tails, ensuring timely protein synthesis necessary for germ cell survival, division, and differentiation. DAZL expression is tightly regulated by epigenetic and post-transcriptional mechanisms and shifts in localization from the nucleus in early spermatogonia to the cytoplasm during meiosis and later stages. Mutations or dysregulation of DAZL are associated with infertility and germ cell developmental abnormalities.

Background

DAZL (Deleted in Azoospermia-Like) is a highly conserved RNA-binding protein that belongs to the DAZ gene family and is essential for germ cell development and gametogenesis in both males and females. It contains two key domains: an RNA Recognition Motif (RRM), approximately 90 amino acids in length, which specifically binds GUU- and UGUU(U/A)-rich sequences in the 3′ untranslated regions (3′UTRs) of target mRNAs, and a DAZ repeat domain, about 24 amino acids long, enriched in asparagine, tyrosine, and glutamine residues. This repeat domain facilitates protein–protein interactions, particularly with other RNA-binding proteins and components of the translation machinery. Through these domains, DAZL forms ribonucleoprotein complexes involved in post-transcriptional regulation. It controls the translation of thousands of mRNAs that govern critical processes in germ cells, including proliferation, meiotic entry, chromatin remodeling, spindle assembly, and synaptonemal complex formation. Depending on the cellular context, DAZL can act as a translational activator or repressor. A major function of DAZL is to recruit poly(A)-binding proteins (PABPs) and translation initiation factors to promote translation of target mRNAs, even those with short poly(A) tails, ensuring timely protein synthesis necessary for germ cell survival, division, and differentiation. DAZL expression is tightly regulated by epigenetic and post-transcriptional mechanisms and shifts in localization from the nucleus in early spermatogonia to the cytoplasm during meiosis and later stages. Mutations or dysregulation of DAZL are associated with infertility and germ cell developmental abnormalities.

References
https://pubmed.ncbi.nlm.nih.gov/40708703/ https://pubmed.ncbi.nlm.nih.gov/26327816/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%