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Anti-CD36 Rabbit Antibody [B18B13]

Catalog No.: F2168

Application: Reactivity: Human

Cited by 1 Publication

Usage Information

Dilution
WB1:1000 - 1:10000 IHC1:1000

Application
WB, IHC

Reactivity
Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
53 kDa 74 kDa, 78 kDa, 88 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human Heart, Human adipose, Human Platelet, Human Hepatocellular cancer, Human cardiac muscle, THP-1 (PMA, 100ng/ml, 72h)
Negative Control	HepG2

Datasheet & SDS

Biological Description

Specificity
CD36 Rabbit mAb detects endogenous levels of total CD36 protein.

Clone
B18B13

Synonym(s)
CD36, GP3B, GP4, Platelet glycoprotein 4, Fatty acid translocase, Glycoprotein IIIb, Leukocyte differentiation antigen CD36, PAS IV, PAS-4, Platelet collagen receptor, Platelet glycoprotein IV, Thrombospondin receptor, FAT, GPIIIB, GPIV

Background
CD36 is a heavily glycosylated integral membrane protein and scavenger receptor widely expressed in various tissues, including adipocytes, myocytes, enterocytes, immune cells, and endothelial cells. Structurally, it features two transmembrane domains, two short intracellular domains at both termini (short cytoplasmic tails), and a large extracellular domain containing a conserved hydrophobic pocket and multiple ligand-binding sites. CD36 functions in high-affinity uptake and sensing of long-chain fatty acids (FAs), facilitating their transport into cells and triggering intracellular signaling via interactions with Src-family kinases. It plays a central role in fatty acid metabolism, including fat taste perception, intestinal absorption, lipoprotein regulation, and mitochondrial β-oxidation. Additionally, CD36 participates in innate immunity, inflammation, angiogenesis, and pathogen recognition, notably serving as a receptor for Plasmodium falciparum-infected erythrocytes. Post-translational modifications such as glycosylation, palmitoylation, and acetylation modulate its localization and function, and mutations or dysregulation of CD36 are implicated in metabolic disorders and susceptibility to diseases like atherosclerosis and malaria.

Background

CD36 is a heavily glycosylated integral membrane protein and scavenger receptor widely expressed in various tissues, including adipocytes, myocytes, enterocytes, immune cells, and endothelial cells. Structurally, it features two transmembrane domains, two short intracellular domains at both termini (short cytoplasmic tails), and a large extracellular domain containing a conserved hydrophobic pocket and multiple ligand-binding sites. CD36 functions in high-affinity uptake and sensing of long-chain fatty acids (FAs), facilitating their transport into cells and triggering intracellular signaling via interactions with Src-family kinases. It plays a central role in fatty acid metabolism, including fat taste perception, intestinal absorption, lipoprotein regulation, and mitochondrial β-oxidation. Additionally, CD36 participates in innate immunity, inflammation, angiogenesis, and pathogen recognition, notably serving as a receptor for Plasmodium falciparum-infected erythrocytes. Post-translational modifications such as glycosylation, palmitoylation, and acetylation modulate its localization and function, and mutations or dysregulation of CD36 are implicated in metabolic disorders and susceptibility to diseases like atherosclerosis and malaria.

References
https://pubmed.ncbi.nlm.nih.gov/24850384/ https://pubmed.ncbi.nlm.nih.gov/27667267/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%