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Anti-CBP Rabbit Antibody [M3F20]

Catalog No.: F0471

Application: Reactivity: Human, Mouse, Rat, Monkey

Usage Information

Dilution
WB1:1000 IP1:200 IF1:100 - 1:200 CHIP1:50

Application
WB, IP, IF, ChIP

Reactivity
Human, Mouse, Rat, Monkey

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
300 kDa

Positive Control	HEK293T; 293; 293T; NIH/3T3; C6; HeLa
Negative Control

Datasheet & SDS

Biological Description

Specificity
CBP Rabbit mAb detects endogenous levels of total CBP protein.

Clone
M3F20

Synonym(s)
CREB-binding protein, Histone lysine acetyltransferase CREBBP, Protein lactyltransferas CREBBP, Protein-lysine acetyltransferase CREBBP, CREBBP, CBP,

Background
CBP (CREB-binding protein) is a large, multifunctional nuclear transcriptional coactivator that interacts with diverse transcription factors, including CREB, c-Fos, c-Jun, c-Myb, and nuclear receptors, to regulate gene expression. Structurally, CBP contains multiple protein–protein interaction domains—such as KIX (CREB-binding), CH1/CH3 (transcription factor binding), bromodomain (acetyl-lysine recognition), and several transcriptional activation domains (TADs) located in both N- and C-terminal regions—along with intrinsic histone acetyltransferase (HAT) activity that remodels chromatin. It is ubiquitously expressed in mammalian cells, functioning as a “signal integrator” at the convergence of various signaling pathways. CBP’s primary role is to bridge sequence-specific transcription factors with the basal transcription machinery (e.g., TBP, TFIIB, RNA Pol II) and to acetylate histones, facilitating transcription initiation. It is essential in processes such as cell growth, differentiation, neuronal plasticity, and homeostasis, with dysregulation linked to developmental disorders (e.g., Rubinstein–Taybi syndrome) and cancers.

Background

CBP (CREB-binding protein) is a large, multifunctional nuclear transcriptional coactivator that interacts with diverse transcription factors, including CREB, c-Fos, c-Jun, c-Myb, and nuclear receptors, to regulate gene expression. Structurally, CBP contains multiple protein–protein interaction domains—such as KIX (CREB-binding), CH1/CH3 (transcription factor binding), bromodomain (acetyl-lysine recognition), and several transcriptional activation domains (TADs) located in both N- and C-terminal regions—along with intrinsic histone acetyltransferase (HAT) activity that remodels chromatin. It is ubiquitously expressed in mammalian cells, functioning as a “signal integrator” at the convergence of various signaling pathways. CBP’s primary role is to bridge sequence-specific transcription factors with the basal transcription machinery (e.g., TBP, TFIIB, RNA Pol II) and to acetylate histones, facilitating transcription initiation. It is essential in processes such as cell growth, differentiation, neuronal plasticity, and homeostasis, with dysregulation linked to developmental disorders (e.g., Rubinstein–Taybi syndrome) and cancers.

References
https://pubmed.ncbi.nlm.nih.gov/8910428/ https://pubmed.ncbi.nlm.nih.gov/10893273/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%