Anti-C1q Rabbit Antibody [G8H3]

Catalog No.: F1660

Application: Reactivity: Mouse

Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain tissue with F1660 at 1:1000 dilution.

Usage Information

Dilution
IHC1:1000

Application
IHC

Reactivity
Mouse

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Positive Control	Mouse cerebral cortex
Negative Control

Datasheet & SDS

Biological Description

Specificity
C1q Rabbit mAb detects endogenous levels of total C1q protein.

Clone
G8H3

Synonym(s)
Complement C1q subcomponent subunit A, C1qa

Background
C1q is a hexameric glycoprotein and the recognition molecule of the classical complement pathway, composed of 18 polypeptide chains forming six heterotrimers (C1qA, C1qB, C1qC), each with a collagen-like N-terminal domain and a globular C-terminal head (gC1q). Mainly produced by cells of the monocyte/macrophage lineage, it is also expressed by dendritic cells, endothelial cells, fibroblasts, and trophoblasts. Structurally resembling a “bouquet of tulips,” C1q plays a central role in innate and adaptive immunity by recognizing immune complexes, apoptotic cells, pathogens, and altered self-antigens via its gC1q domain, while its collagen domain interacts with receptors and serine proteases (C1r/C1s) to initiate the classical complement cascade. Beyond its complement-activating function, C1q performs diverse non-canonical roles including clearance of apoptotic cells, regulation of inflammation, neurodevelopment via synaptic pruning, pro-angiogenic activity in pregnancy and wound healing, tumor surveillance, and potential involvement in aging and neurodegeneration.

Background

C1q is a hexameric glycoprotein and the recognition molecule of the classical complement pathway, composed of 18 polypeptide chains forming six heterotrimers (C1qA, C1qB, C1qC), each with a collagen-like N-terminal domain and a globular C-terminal head (gC1q). Mainly produced by cells of the monocyte/macrophage lineage, it is also expressed by dendritic cells, endothelial cells, fibroblasts, and trophoblasts. Structurally resembling a “bouquet of tulips,” C1q plays a central role in innate and adaptive immunity by recognizing immune complexes, apoptotic cells, pathogens, and altered self-antigens via its gC1q domain, while its collagen domain interacts with receptors and serine proteases (C1r/C1s) to initiate the classical complement cascade. Beyond its complement-activating function, C1q performs diverse non-canonical roles including clearance of apoptotic cells, regulation of inflammation, neurodevelopment via synaptic pruning, pro-angiogenic activity in pregnancy and wound healing, tumor surveillance, and potential involvement in aging and neurodegeneration.

References
https://pubmed.ncbi.nlm.nih.gov/26175731/ https://pubmed.ncbi.nlm.nih.gov/29692784/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%