Anti-BMP-6 Mouse Antibody [N23M12]

Catalog No.: F2722

Application: Reactivity: Human

Usage Information

Dilution
WB1:1000

Application
WB, ELISA

Reactivity
Human

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Datasheet & SDS

Biological Description

Specificity
BMP-6 Mouse mAb detects endogenous levels of total BMP6 protein.

Clone
N23M12

Synonym(s)
Bone morphogenetic protein 6, BMP-6, VG-1-related protein , VG-1-R, VGR-1, VGR

Background
BMP-6 (Bone Morphogenetic Protein-6) is a member of the TGF-β superfamily, produced as an inactive pre-pro-polypeptide comprising an N-terminal signal peptide, a pro-domain, and a C-terminal mature domain containing seven cysteines—six forming intramolecular disulfide bonds and one mediating covalent dimerization to generate the active ligand. It is expressed in multiple embryonic and adult tissues, including developing limb buds, bone, cartilage, ovaries, liver, and the central nervous system. BMP-6 plays crucial roles in skeletal biology by promoting endochondral ossification, fracture healing, and cartilage repair, and in non-skeletal functions such as regulating hepatic hepcidin expression, thereby controlling systemic iron homeostasis. In the lung, BMP-6 supports normal function and modulates inflammation, with reduced expression linked to cigarette smoke exposure, COPD progression, and altered iron metabolism.

Background

BMP-6 (Bone Morphogenetic Protein-6) is a member of the TGF-β superfamily, produced as an inactive pre-pro-polypeptide comprising an N-terminal signal peptide, a pro-domain, and a C-terminal mature domain containing seven cysteines—six forming intramolecular disulfide bonds and one mediating covalent dimerization to generate the active ligand. It is expressed in multiple embryonic and adult tissues, including developing limb buds, bone, cartilage, ovaries, liver, and the central nervous system. BMP-6 plays crucial roles in skeletal biology by promoting endochondral ossification, fracture healing, and cartilage repair, and in non-skeletal functions such as regulating hepatic hepcidin expression, thereby controlling systemic iron homeostasis. In the lung, BMP-6 supports normal function and modulates inflammation, with reduced expression linked to cigarette smoke exposure, COPD progression, and altered iron metabolism.

References
https://pubmed.ncbi.nlm.nih.gov/30542109/ https://pubmed.ncbi.nlm.nih.gov/27252362/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%