Catalog No.S3643 Synonyms: NSC 324552

For research use only.

Amitraz (NSC 324552) is a triazapentadiene, an α2 adrenergic agonist and a member of the amidine chemical family. It is a non-systemic acaricide and insecticide.

Amitraz Chemical Structure

CAS No. 33089-61-1

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Biological Activity

Description Amitraz (NSC 324552) is a triazapentadiene, an α2 adrenergic agonist and a member of the amidine chemical family. It is a non-systemic acaricide and insecticide.
α2 adrenergic receptor [1]
In vitro

Amitraz was not cytotoxic to human luteinized granulosa cells at concentrations of 1, 10 or 50 µg/ml for exposures of 2-72 h While the highest concentration of amitraz tested, 100 µg/ml, caused significant cell death after exposures of 24, 48 and 72 h. Amitraz decreased the amount of progesterone produced by each cell after a 4 h exposure; the reduction in progesterone concentration was not caused by decreased cell viability[1].

In vivo Amitraz is an α2-adrenergic receptor (α2-AR) agonist that adversely affects the mammalian reproductive system by binding to presynaptic α2-AR in the hypothalamus, thus inhibiting noradrenalin release and decreasing GnRH secretion. When 30 mg/kg of amitraz was administered to rats, the ovulatory LH surge was prevented. Amitraz inhibited insulin but stimulated glucagon secretion in a perfused rat pancreas model. Amitraz also decreased intestinal motility, and inhibited prostaglandin synthesis by bovine seminal vesicle microsomes. Amitraz can be absorbed through the skin and can exert systemic effects via the vascular system in humans[1]. Low doses of AMZ (l-25 mg/kg) decreased motor activity and altered the rates and patterns of responding under schedule-controlled conditions. Intermediate doses (50-100 mg/kg) affect visual-evoked potentials and lower body weight and temperature. In addition, 100 mg/kg produces slight MAO inhibition and characteristic signs of intoxication (e.g., weight loss and hyperreactivity). High doses (>lOO mg/kg) produce more pronounced MAO inhibition, extreme weight loss and hyperreactivity, aggression, and lethality[2].

Protocol (from reference)

Cell Research:


  • Cell lines: Luteinized human granulosa cells
  • Concentrations: 1, 10, 50 and 100 µg/ml
  • Incubation Time: 2, 4, 6, 24, 48 or 72 h
  • Method:

    Cells were exposed to 1, 10, 50 and 100 µg/ml amitraz for 2, 4, 6, 24, 48 or 72 h.

  • (Only for Reference)
Animal Research:


  • Animal Models: Adult male Long-Evans hooded rats
  • Dosages: 1-200 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 58 mg/mL
(197.67 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 293.41


CAS No. 33089-61-1
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=CC(=C(C=C1)N=CN(C)C=NC2=C(C=C(C=C2)C)C)C

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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