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AMH Rabbit mAb

Catalog No.: F2573

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 IP1:30 IHC1:2000

Application
WB, IP, IHC

Source
Rabbit

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
60 kDa 59 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
AMH Rabbit mAb detects endogenous levels of total AMH protein.

Clone
K2B16

Synonym(s)
Muellerian-inhibiting factor, Anti-Muellerian hormone, Muellerian-inhibiting substance, AMH, MIS, Amh.

Background
Anti-Müllerian hormone (AMH), also known as Müllerian inhibiting factor or Müllerian inhibiting substance, plays diverse and complex roles in the development and function of various human tissues. AMH is a glycoprotein belonging to the transforming growth factor beta (TGFβ) superfamily, which comprises 33 signaling proteins, including activins, inhibin A and B, bone morphogenetic proteins (BMPs), and growth differentiation factors (GDFs) such as myostatin. The AMH protein consists of two main parts: an N-terminal prodomain and a C-terminal growth factor (GF) domain. It is encoded by a gene located on the short arm of human chromosome 19, containing 5 exons and 4 introns. The gene’s regulatory regions feature binding sites for multiple transcription factors, notably SOX9 (SRY-box transcription factor 9) and NFκB (nuclear factor kappa B). Members of the TGFβ superfamily signal through seven type I and five type II receptors; however, AMH specifically binds only to its type II receptor, AMHR2. Consequently, only cells expressing AMHR2 can directly respond to AMH stimulation. In terms of expression, AMH is produced by Sertoli cells in the testes, granulosa cells (GCs) in preantral and small antral follicles of the ovaries, and the endometrium of women of reproductive age. It is also expressed in motoneurons, gonadotropin-releasing hormone (GnRH) neurons, and the hippocampus, as well as in certain tumors, including endometrial cancer (EC), sex cord-stromal tumors, and granulosa cell tumors. Additionally, trace amounts of AMH are detectable in skeletal muscles, the sciatic nerve, the spinal cord, and the mouse brain.

Background

Anti-Müllerian hormone (AMH), also known as Müllerian inhibiting factor or Müllerian inhibiting substance, plays diverse and complex roles in the development and function of various human tissues. AMH is a glycoprotein belonging to the transforming growth factor beta (TGFβ) superfamily, which comprises 33 signaling proteins, including activins, inhibin A and B, bone morphogenetic proteins (BMPs), and growth differentiation factors (GDFs) such as myostatin. The AMH protein consists of two main parts: an N-terminal prodomain and a C-terminal growth factor (GF) domain. It is encoded by a gene located on the short arm of human chromosome 19, containing 5 exons and 4 introns. The gene’s regulatory regions feature binding sites for multiple transcription factors, notably SOX9 (SRY-box transcription factor 9) and NFκB (nuclear factor kappa B). Members of the TGFβ superfamily signal through seven type I and five type II receptors; however, AMH specifically binds only to its type II receptor, AMHR2. Consequently, only cells expressing AMHR2 can directly respond to AMH stimulation. In terms of expression, AMH is produced by Sertoli cells in the testes, granulosa cells (GCs) in preantral and small antral follicles of the ovaries, and the endometrium of women of reproductive age. It is also expressed in motoneurons, gonadotropin-releasing hormone (GnRH) neurons, and the hippocampus, as well as in certain tumors, including endometrial cancer (EC), sex cord-stromal tumors, and granulosa cell tumors. Additionally, trace amounts of AMH are detectable in skeletal muscles, the sciatic nerve, the spinal cord, and the mouse brain.

References
[1] Gowkielewicz M, et al. Front Endocrinol (Lausanne). 2024 Sep 16:15:1468364.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%