Choose Your Country or Region

Acetyl-Histone H4 (Lys5) Rabbit mAb

Catalog No.: F1090

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:500000 IP1:30 IHC1:500 IF1:5000

Application
WB, IP, IHC, IF, ChIP, ELISA

Source
Rabbit

Reactivity
Mouse, Rat, Human

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
11 kDa 11 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Datasheet & SDS

Biological Description

Specificity
Acetyl-Histone H4 (Lys5) Rabbit mAb detects endogenous levels of Histone H4 only when Acetylated at Lys5.

Clone
K4A18

Synonym(s)
H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H

Background
Acetyl-Histone H4 (Lys5) refers to the post-translationally modified form of histone H4 in which the lysine residue at position 5 on its N-terminal tail is acetylated. Histone H4 is a core component of the nucleosome, the fundamental unit of chromatin in eukaryotic cells, and consists of a globular histone fold domain for nucleosome assembly and an N-terminal tail rich in lysine residues (including Lys5, Lys8, Lys12, and Lys16) that are targets for dynamic acetylation. Acetylation at Lys5, one of the earliest histone H4 modifications, neutralizes the positive charge of the lysine side chain, reducing the histone's affinity for negatively charged DNA. This leads to a relaxation of chromatin structure, facilitating key nuclear processes such as transcription, DNA replication, and DNA repair. Lys5 acetylation is associated with active chromatin states, partly through its role in recruiting bromodomain-containing proteins and chromatin remodeling complexes. This mark frequently occurs alongside Lys12 acetylation during nucleosome assembly, suggesting a coordinated mechanism for regulating chromatin maturation and epigenetic memory. Dysregulation of histone H4 Lys5 acetylation is linked to developmental disorders and cancer.

Background

Acetyl-Histone H4 (Lys5) refers to the post-translationally modified form of histone H4 in which the lysine residue at position 5 on its N-terminal tail is acetylated. Histone H4 is a core component of the nucleosome, the fundamental unit of chromatin in eukaryotic cells, and consists of a globular histone fold domain for nucleosome assembly and an N-terminal tail rich in lysine residues (including Lys5, Lys8, Lys12, and Lys16) that are targets for dynamic acetylation. Acetylation at Lys5, one of the earliest histone H4 modifications, neutralizes the positive charge of the lysine side chain, reducing the histone's affinity for negatively charged DNA. This leads to a relaxation of chromatin structure, facilitating key nuclear processes such as transcription, DNA replication, and DNA repair. Lys5 acetylation is associated with active chromatin states, partly through its role in recruiting bromodomain-containing proteins and chromatin remodeling complexes. This mark frequently occurs alongside Lys12 acetylation during nucleosome assembly, suggesting a coordinated mechanism for regulating chromatin maturation and epigenetic memory. Dysregulation of histone H4 Lys5 acetylation is linked to developmental disorders and cancer.

References
[1] Owen DJ, et al. EMBO J. 2000 Nov 15;19(22):6141-9. [2] Zhang K, et al. Mol Cell Proteomics. 2002 Jul;1(7):500-8.

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%