Luteolin

For research use only.

Catalog No.S2320 Synonyms: Luteoline, Luteolol, Digitoflavone

14 publications

Luteolin Chemical Structure

CAS No. 491-70-3

Luteolin (Luteoline, Luteolol, Digitoflavone) is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.

Selleck's Luteolin has been cited by 14 publications

1 Customer Review

  • Bioactive compounds inhibit recombinant HDAC activity. Recombinant HDACs were incubated with flavonoid Luteolin (C) for 2 h prior to the addition of cell permeable HDAC substrates. Luteolin inhibited activity of most classes I, IIa, and IIb HDAC isoforms.

    Mol Nutr Food Res, 2017, doi: 10.1002/mnfr.201600744. Luteolin purchased from Selleck.

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Biological Activity

Description Luteolin (Luteoline, Luteolol, Digitoflavone) is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.
Targets
PDE2 [1]
(Cell-free assay)
PDE5 [1]
(Cell-free assay)
PDE4 [1]
(Cell-free assay)
PDE3 [1]
(Cell-free assay)
PDE1 [1]
(Cell-free assay)
6.4 μM(Ki) 9.5 μM(Ki) 11.1 μM(Ki) 13.9 μM(Ki) 15.0 μM(Ki)
In vitro

Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. [1] Luteolin inhibits LPS-stimulated TNF-alpha production with an IC50 of less than 1 μM. Luteolin inhibites LPS-induced phosphorylation of Akt as well as IkappaBalpha. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells MXrGeY5kfGmxbjDhd5NigQ>? NITPVVEzKGh? NF23cplKdmirYnn0bY9vKG:oIF7PJJBzd2S3Y4Tpc44hcW5iTGDTMZN1cW23bHH0[YQhdW:3c3WgVmFYOjZ2LkegZ4VtdHNicILlMYlv[3WkYYTl[EBnd3JiMjDodpMh[mWob4LlJGxRWyC|dHnteYxifGmxbjDmc5IhOjRiaILzJIJ6KEe{aXXzd{Bie3OjeTDt[ZRpd2RuIFnDOVA:OC5{MTFOwG0> MknHNlUyPzZzOEe=
HEK293 FS cells MUfGeY5kfGmxbjDhd5NigQ>? MUnJcohq[mm2aX;uJI9nKE6RWESg[ZhxemW|c3XkJIlvKEiHS{K5N{BHWyClZXzsd{Bie3Onc4Pl[EBieyCKMl:yJJBzd2S3Y4Tpc44h[nliSELPNk9VgXJxTGDPJIF{e2G7LDDJR|UxRTBwOEWg{txO NV\6bXI1OjB5M{GzOVc>
CHO cells NYLjSndWTnWwY4Tpc44h[XO|YYm= MWHB[49vcXO2IHHjeIl3cXS7IHH0JJJifCCGQWSg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiRVO1NF0yNjR3IN88US=> M1\FSlIxQTdzNkWw
human MV4-11 cells MY\DfZRwfG:6aXPpeJkh[XO|YYm= NHHqe5M4OiCq NYPaeoZ7S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVVZ2LUGxJINmdGy|IHjhdoJwemmwZzDGUHQ{KG23dHH0bY9vKGGodHXyJFczKGi{czDifUB1\XS{YYrvcIl2dSCkYYPl[EBGgiCFeWTvfEBk\WyuII\pZYJqdGm2eTDhd5NigSxiR1m1NF0yNjd4IN88US=> MU[yN|QyOTB5Mx?=
HEK293 cells M2LFeGZ2dmO2aX;uJIF{e2G7 NGSxPXMzPCCq NWHueVVYSWexbnnzeEBi[3Srdnn0fUBifCCvb4Xz[UBRWEGUZ3HtcYEh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bkdy2neIDy[ZN{cW6pIIfpeIghT2GuNDDy[ZBwenSncjD2[YN1d3JiYX\0[ZIhOjRiaILzJIJ6KGS3YXytcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeTygSWM2OD1{LkOg{txO M1zET|I1QTV3OEi5
human U2OS cells MlHxSpVv[3Srb36gZZN{[Xl? NFi1UIM2KGh? Mo\WRYdwdmm|dDDhZ5Rqfmm2eTDheEBIWFJ|NTDy[YNmeHSxcjDpckBpfW2jbjDVNm9UKGOnbHzzJINw\XiycnXzd4lv\yCJYXy0MXZROTZvVFXWJIF{e2W|c3XkJIF{KGKndHGgZZJz\XO2aX6geJJidnOub3PheIlwdiCjZoTldkA2KGi{czDifUBj\XSjIHzhZ5RidWG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUOuNkDPxE1? NXnURmV7OjR7MEC0OFc>
RBL-2H3 cells M3vO[WZ2dmO2aX;uJIF{e2G7 NUjTXXZTUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEmOLUSgdJJw\HWldHnvckBqdiCUQlytNmg{KGOnbHzzJJdieyCmZYTldo1qdmWmLDDJR|UxRTNwNzFOwG0> NWXzd|NtOTJ7NUGwPVI>
human mast cells MXnGeY5kfGmxbjDhd5NigQ>? MnT0TY5pcWKrdHnvckBw\iCVWVugbY4hcHWvYX6gcYF{fCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gcYF{fCClZXzsJIRm\3KjboXsZZRqd25uIFXDOVA:PC53IN88US=> NG\nUmMzOjJ3N{KxNy=>
human LNCAP cells M3\0OGZ2dmO2aX;uJIF{e2G7 MXHEc5dvemWpdXzheIlwdiCxZjDwdo9{fGG2ZTDzdIVkcW[rYzDhcpRq\2WwIIPlZ5JmfGmxbjDpckBpfW2jbjDMUmNCWCClZXzsd{whUUN3ME21JO69VQ>? NGrXc|IzPTBzOUS3PC=>
rat H9c2 cells NETQWlRHfW6ldHnvckBie3OjeR?= NG\FfmwzPCCq M1nSb2N6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RiZH;4c5J2[mmlaX6tbY5lfWOnZDDjfZRwfG:6aXPpeJkhcW5icnH0JGg6[zJiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhTUN3ME21MlU{KM7:TR?= MWKyNFk{Ojd4Mh?=
human HT-29 cells MmCySpVv[3Srb36gZZN{[Xl? MVSxNEBucW6| NWHGPXZ5SWexbnnzeEBi[3Srdnn0fUBifCCJUGKzOUBz\WOncITvdkBqdiCqdX3hckBJXC1{OTDj[YxteyCjZoTldkAyOCCvaX7zJIJ6KGS7bnHtbYMhdWG|czDy[YRqe3S{aXL1eIlwdiCjc4PhfUwhTUN3ME23MlI1KM7:TR?= M2XPRVI1QTByNES3
human RS4:11 cells M{fCfGN6fG:2b4jpZ4l1gSCjc4PhfS=> NXHh[mtNPzJiaB?= NXrudlVqS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWlN2OkGxJINmdGy|IHjhdoJwemmwZzD3bYxlKHS7cHWgSmxVOyCjZoTldkA4OiCqcoOgZpkhfGW2cnH6c4xqfW1iYnHz[YQhTXpiQ4nUc5gh[2WubDD2bYFjcWyrdImgZZN{[XluIFfJOVA:Py5{NTFOwG0> NFniT2QzOzRzMUC3Ny=>
NCI-H460 cells NG\nRXZHfW6ldHnvckBie3OjeR?= NEfVUHQzNTJyIHi= M4qzRmlvcGmkaYTpc44hd2ZiQVLDS|Ih\XiycnXzd4VlKGmwIHj1cYFvKE6FST3IOFYxKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gVIhCKGGlY4XteYxifGmxbjDh[pRmeiB{IITvJFIxKGi{czDy[YxifGm4ZTD0c{BnfW2rdILlcY9z\2mwIFOsJGlEPTB;OD65JO69VQ>? M2i3R|IyOjd3M{i2
human H9 cells MnfHSpVv[3Srb36gZZN{[Xl? M{nLflMh\GG7cx?= NGfWS5lCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKGi3bXHuJGg6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IICyOEBidnSrZ3XuJINieHS3cnWgZZN{[XluIFXDOVA:OTBizszN MWS4NVU5OTZ2
MDCK cells MnfFR5l1d3SxeHnjbZR6KGG|c3H5 NWS4UXVYS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTDT{Bk\WyuczDifUBOXFRiYYPzZZktKEOFNUC9NVIvPDRizszN NV\ZZm5SOTh4NECwOFI>
mouse B16-4A5 cells MlO4SpVv[3Srb36gZZN{[Xl? MmDvO|IhcA>? MVHJcohq[mm2aX;uJI9nKHSqZX;wbJltdGmwZT3zeIlufWyjdHXkJI1mdGGwb3flcoV{cXNiaX6gcY92e2ViQkG2MVRCPSClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:OTRizszN NIHKN|kzODF6OUO5PS=>
K562 cells MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1TwTVUh\GG7cx?= MWLHdo94fGhiaX7obYJqfGmxbjDv[kBMPTZ{IHPlcIx{KGK7IGjUWEBie3OjeTDh[pRmeiB3IHThfZMtKEmFNUC9NVQvPjVizszN NIe2[|kyPzRzMUC5Ni=>
human H9 cells M{LIb2N6fG:2b4jpZ4l1gSCjc4PhfS=> NGmxPXE{KGSjeYO= NVzQUld6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUDliY3XscJMh[W[2ZYKgN{Bl[Xm|LDDJR|UxRTF4IN88US=> MVu4NVU5OTZ2
human HT-29 cells NH3MRnBHfW6ldHnvckBie3OjeR?= NVv4bWFkOTBibXnudy=> MorISIV{\W6|aYTpfoF1cW:wIH;mJGdRWjN3IILlZ4VxfG:{IHnuJIh2dWGwIFjUMVI6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gfoFxemmwYYP0MYlv\HWlZXSg[Jlv[W2rYzDtZZN{KHKnZHnzeJJq[nW2aX;uJIFnfGW{IEGwJI1qdnNuIFnDOVA:OThwNjFOwG0> M37ZVFI1QTByNES3
human U937 cells NYnVTGdCWHKxbHnm[ZJifGmxbjDhd5NigQ>? NGH2T|U4OiCq NEf2O2NCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGW5N|ch[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGfTWE05KGG|c3H5MEBKSzVyPUKwJO69VQ>? NEHrbYMyPzF3OEC1OC=>
mouse HT22 cells Mn\2SpVv[3Srb36gZZN{[Xl? M1jOXlMhcA>? MYHO[ZVzd3C{b4TlZ5RqfmViYXP0bZZqfHliaX6gcY92e2ViSGSyNkBk\WyuczDhd5Nme3OnZDDhd{B1NUKRT1itbY5lfWOnZDD0c5hq[2m2eTDheEA1OCC3TTDwdoVqdmO3YnH0[YQh\m:{IEOgbJJ{KG[xbHzve4VlKGK7IIStRm9QUCCrbnT1Z5Rqd25ibXXhd5Vz\WRiYX\0[ZIhQSCqcoOgZpkhVVSWIHHzd4F6 MViyOFI1PTl|OR?=
human THP1 cells Ml;YSpVv[3Srb36gZZN{[Xl? M3jObFIxKM7:TR?= MlP1NUBp NVj5NVVWTG:5boLl[5Vt[XSrb36gc4YhXFCDLXnu[JVk\WRiTl;YNkBuWk6DIHX4dJJme3Orb36gbY4hcHWvYX6gWGhROSClZXzsd{BifCB{MDD1UUBqdmO3YnH0[YQh\m:{IEGgbJIheHKrb4KgeI8hXFCDIHPoZYxt\W6pZTDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliUmStVGNTKGGwYXz5d4l{ NVjRRmtQOjN5OE[1NlA>
MDA-MB-231 cells MoK1SpVv[3Srb36gZZN{[Xl? NUHPUIlZPSEQvF2= NVvqSZhoOTZiaB?= NHfyNpZKdmirYnn0bY9vKG:oIGDNRU1{fGmvdXzheIVlKE6ILXvhdJBiSiC|aXfuZYxqdmdiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gUWRCNU2ELUKzNUBk\WyuczDheEA2KHWPIHnuZ5Vj[XSnZDDmc5IhOTZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?= MojoNlUyQTB2Nk[=
HL60 cells MVnQdo9tcW[ncnH0bY9vKGG|c3H5 MX6zNEDPxE1? MVi0PEBp M{PPNGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFy2NEBk\WyuczDheEA{OCC3TTDh[pRmeiB2ODDodpMh[nliTWTTJIF{e2G7 MoP6NVgzPTh2NEC=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FADD / PARP / Cleaved PARP ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of FADD, PARP, and cleaved PARP were then measured by Western blot analysis.

Caspase-3 / Cleaved Caspase-3 / Caspase-8 / Cleaved Caspase-8 ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of Caspase-8, cleaved Caspase-8, Caspase-3, and cleaved Caspase-3 were measured by Western blot analysis. 

ERK / p-ERK / JNK / p-JNK / p38 / p-p38 / Bax / Bcl-2 ; 

PubMed: 30992674     


LN229 cells were treated with luteolin (0, 10, 20, and 30 µM) for 24 hours, and Western blot analysis was performed to measure the expression levels of ERK, P-ERK, JNK, P-JNK, p38, P-p38, Bax, and Bcl-2. 

p-VEGFR2 / p-mTOR / pS6K1 / p70S6K1 / pAKT / AKT / MMP-2 / MMP-9 ; 

PubMed: 23300633     


Luteolin suppressed the activation of VEGFR2 and their downstream signaling pathway triggered by VEGF in HUVECs. Proteins from different treatments was tested by Western blotting and probed with specific antibodies. Experiments were repeated for three times. 

p21 / Survivin / Cyclin D1; 

PubMed: 30930986     


Total protein was isolated from control and luteolin-treated MDA-MB-231 cells, and subjected to immunoblotting of p21, Survivin and cyclin D1 proteins. Membranes were stripped and re-probed with anti-GAPDH antibody to ensure equal protein loading. p21 was upregulated, whereas Survivin and cyclin D1 were downregulated in MDA-MB-231 cell in a dose-dependent manner. *P<0.05 vs. the control group (0 µM luteolin); #P<0.05 vs. the 10 µM luteolin group.

DNMT1 / DMNT3A / DNMT3B ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against DNMT1, DNMT3A, DNMT3B.

TET1 / TET2 / TET3 ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against TET1, TET2, and TET3.

30992674 23300633 30930986 30988303
Immunofluorescence
5-hmC ; 

PubMed: 30988303     


Formation of 5-hmC by TETs was assessed using confocal imaging.

30988303
Growth inhibition assay
Cell viability; 

PubMed: 30992674     


(B) LN229 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (C) U251 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (D) HEB cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay.

30992674
In vivo LD50: Mice >2500mg/kg (i.g.) [3]

Protocol

Solubility (25°C)

In vitro DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL warmed (10.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 286.24
Formula

C15H10O6

CAS No. 491-70-3
Storage powder
in solvent
Synonyms Luteoline, Luteolol, Digitoflavone
Smiles C1=CC(=C(C=C1C2=CC(=O)C3=C(C=C(C=C3O2)O)O)O)O

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    Would you please suggest a suitable vehicle to dissolve Luteolin for in vivo i.p. use?

  • Answer:

    Formula: 5% DMSO+40% PEG 300+5% Tween80+ddH2O, working Solution concentration: up to 7.5mg/ml, stable for 30min.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID