Luteolin

Catalog No.S2320

Luteolin Chemical Structure

Molecular Weight(MW): 286.24

Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.

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Cited by 6 Publications

1 Customer Review

  • Bioactive compounds inhibit recombinant HDAC activity. Recombinant HDACs were incubated with flavonoid Luteolin (C) for 2 h prior to the addition of cell permeable HDAC substrates. Luteolin inhibited activity of most classes I, IIa, and IIb HDAC isoforms.

    Mol Nutr Food Res, 2017, doi: 10.1002/mnfr.201600744. Luteolin purchased from Selleck.

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Biological Activity

Description Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.
Targets
PDE2 [1]
(Cell-free assay)
PDE5 [1]
(Cell-free assay)
PDE4 [1]
(Cell-free assay)
PDE3 [1]
(Cell-free assay)
PDE1 [1]
(Cell-free assay)
6.4 μM(Ki) 9.5 μM(Ki) 11.1 μM(Ki) 13.9 μM(Ki) 15.0 μM(Ki)
In vitro

Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. [1] Luteolin inhibits LPS-stimulated TNF-alpha production with an IC50 of less than 1 μM. Luteolin inhibites LPS-induced phosphorylation of Akt as well as IkappaBalpha. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NVXQVmQ6TnWwY4Tpc44h[XO|YYm= MVuyJIg> MoLzTY5pcWKrdHnvckBw\iCQTzDwdo9lfWO2aX;uJIlvKEySUz3zeIlufWyjdHXkJI1wfXOnIGLBW|I3PC55IHPlcIx{KHC{ZT3pcoN2[mG2ZXSg[o9zKDJiaILzJIJm\m:{ZTDMVHMhe3SrbYXsZZRqd25iZn;yJFI1KGi{czDifUBIemmnc4OgZZN{[XlibXX0bI9lNCCLQ{WwQVAvOjFizszN NIDOSHMzPTF5NkG4Oy=>
HEK293 FS cells NFvET5hHfW6ldHnvckBie3OjeR?= MXXJcohq[mm2aX;uJI9nKE6RWESg[ZhxemW|c3XkJIlvKEiHS{K5N{BHWyClZXzsd{Bie3Onc4Pl[EBieyCKMl:yJJBzd2S3Y4Tpc44h[nliSELPNk9VgXJxTGDPJIF{e2G7LDDJR|UxRTBwOEWg{txO NIOzTYozODd|MUO1Oy=>
CHO cells MXrGeY5kfGmxbjDhd5NigQ>? MljXRYdwdmm|dDDhZ5Rqfmm2eTDheEBz[XRiRFHUJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFXDOVA:OS52NTFOwG0> M3;nW|IxQTdzNkWw
human MV4-11 cells M1nibGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGrsU4g4OiCq NYOxVnNWS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVVZ2LUGxJINmdGy|IHjhdoJwemmwZzDGUHQ{KG23dHH0bY9vKGGodHXyJFczKGi{czDifUB1\XS{YYrvcIl2dSCkYYPl[EBGgiCFeWTvfEBk\WyuII\pZYJqdGm2eTDhd5NigSxiR1m1NF0yNjd4IN88US=> MVKyN|QyOTB5Mx?=
HEK293 cells MYfGeY5kfGmxbjDhd5NigQ>? MVmyOEBp MnX6RYdwdmm|dDDhZ5Rqfmm2eTDheEBud3W|ZTDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDjc{1mgHC{ZYPzbY5oKHerdHigS4FtPCC{ZYDvdpRmeiC4ZXP0c5Ih[W[2ZYKgNlQhcHK|IHL5JIR2[WxvbIXjbYZmemG|ZTDy[ZBwenSncjDhd5NigSxiRVO1NF0zNjNizszN M{\ZflI1QTV3OEi5
human U2OS cells NYXZU4tUTnWwY4Tpc44h[XO|YYm= M2nLZ|UhcA>? NILoU2lC\2:waYP0JIFkfGm4aYT5JIF1KEeSUkO1JJJm[2WydH;yJIlvKGi3bXHuJHUzV1NiY3XscJMh[2:neIDy[ZN{cW6pIFfhcFQuXlBzNj3USXYh[XO|ZYPz[YQh[XNiYnX0ZUBienKnc4TpckB1emGwc3zvZ4F1cW:wIHHmeIVzKDViaILzJIJ6KGKndHGgcIFkfGGvYYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9N{4zKM7:TR?= M{PT[VI1QTByNES3
RBL-2H3 cells NIjDR2FHfW6ldHnvckBie3OjeR?= NH:wNXRKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUUxvNDDwdo9lfWO2aX;uJIlvKFKETD2yTFMh[2WubIOge4F{KGSndHXycYlv\WRuIFnDOVA:Oy55IN88US=> MkDjNVI6PTFyOUK=
human mast cells NXK3T2xoTnWwY4Tpc44h[XO|YYm= M4noO2lvcGmkaYTpc44hd2ZiU2nLJIlvKGi3bXHuJI1ie3RiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJI1ie3RiY3XscEBl\We{YX71cIF1cW:wLDDFR|UxRTRwNTFOwG0> MYWyNlI2PzJzMx?=
human LNCAP cells MnvnSpVv[3Srb36gZZN{[Xl? M4Xw[2Rwf26{ZXf1cIF1cW:wIH;mJJBzd3O2YYTlJJNx\WOrZnnjJIFvfGmpZX6gd4VkemW2aX;uJIlvKGi3bXHuJGxPS0GSIHPlcIx{NCCLQ{WwQVUh|ryP NF\0eIwzPTBzOUS3PC=>
rat H9c2 cells M3fZU2Z2dmO2aX;uJIF{e2G7 M1XmOlI1KGh? M{PYemN6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RiZH;4c5J2[mmlaX6tbY5lfWOnZDDjfZRwfG:6aXPpeJkhcW5icnH0JGg6[zJiY3XscJMh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhTUN3ME21MlU{KM7:TR?= MnHkNlA6OzJ5NkK=
human HT-29 cells NYnrWmlrTnWwY4Tpc44h[XO|YYm= MkjFNVAhdWmwcx?= MkjORYdwdmm|dDDhZ5Rqfmm2eTDheEBIWFJ|NTDy[YNmeHSxcjDpckBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjCxNEBucW6|IHL5JIR6dmGvaXOgcYF{eyC{ZXTpd5RzcWK3dHnvckBie3OjeTygSWM2OD15LkK0JO69VQ>? M2L4WFI1QTByNES3
human RS4:11 cells MV\DfZRwfG:6aXPpeJkh[XO|YYm= MXO3NkBp MmjzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVnM1QjFzIHPlcIx{KGijcnLvdolv\yC5aXzkJJR6eGViRlzUN{Bi\nSncjC3NkBpenNiYomgeIV1emG8b3zpeY0h[mG|ZXSgSZohS3mWb4igZ4VtdCC4aXHibYxqfHliYYPzZZktKEeLNUC9O{4zPSEQvF2= M4PGT|I{PDFzMEez
NCI-H460 cells MVjGeY5kfGmxbjDhd5NigQ>? MYiyMVIxKGh? MXvJcohq[mm2aX;uJI9nKEGEQ1eyJIV5eHKnc4Pl[EBqdiCqdX3hckBPS0lvSES2NEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGDoRUBi[2O3bYXsZZRqd25iYX\0[ZIhOiC2bzCyNEBpenNicnXsZZRqfmVidH:g[pVucXS{ZX3vdodqdiCFLDDJR|UxRThwOTFOwG0> MmOwNlEzPzV|OE[=
human H9 cells MlX0SpVv[3Srb36gZZN{[Xl? MXmzJIRigXN? M1HuXWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhKXjFiM1KgbY5n\WO2ZXSgbY4hcHWvYX6gTFkh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDh[pRmeiB|IHThfZMh[nlicEK0JIFvfGmpZX6gZ4FxfHW{ZTDhd5NigSxiRVO1NF0yOCEQvF2= MXG4NVU5OTZ2
MDCK cells NEHkenhEgXSxdH;4bYNqfHliYYPzZZk> NXTiSmd7S3m2b4TvfIlkcXS7IHHnZYlve3RiTVTDT{Bk\WyuczDifUBOXFRiYYPzZZktKEOFNUC9NVIvPDRizszN M37n[VE5PjRyMESy
mouse B16-4A5 cells M1:4NGZ2dmO2aX;uJIF{e2G7 NY\VdJpvPzJiaB?= MUnJcohq[mm2aX;uJI9nKHSqZX;wbJltdGmwZT3zeIlufWyjdHXkJI1mdGGwb3flcoV{cXNiaX6gcY92e2ViQkG2MVRCPSClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:OTRizszN NI\aZ3MzODF6OUO5PS=>
K562 cells M2jxUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIXnSXY2KGSjeYO= MXrHdo94fGhiaX7obYJqfGmxbjDv[kBMPTZ{IHPlcIx{KGK7IGjUWEBie3OjeTDh[pRmeiB3IHThfZMtKEmFNUC9NVQvPjVizszN Mlj1NVc1OTFyOUK=
human H9 cells MlKwR5l1d3SxeHnjbZR6KGG|c3H5 MlHCN{Bl[Xm| MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIPUBk\WyuczDh[pRmeiB|IHThfZMtKEmFNUC9NVYh|ryP MXG4NVU5OTZ2
human HT-29 cells M1;DRWZ2dmO2aX;uJIF{e2G7 M2SwUlExKG2rboO= M1G4VGRme2Wwc3n0bZpifGmxbjDv[kBIWFJ|NTDy[YNmeHSxcjDpckBpfW2jbjDIWE0zQSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJpieHKrbnHzeE1qdmS3Y3XkJIR6dmGvaXOgcYF{eyC{ZXTpd5RzcWK3dHnvckBi\nSncjCxNEBucW6|LDDJR|UxRTF6Lk[g{txO M{n5d|I1QTByNES3
human U937 cells MlzOVJJwdGmoZYLheIlwdiCjc4PhfS=> MXm3NkBp NH7J[IFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGW5N|ch[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGfTWE05KGG|c3H5MEBKSzVyPUKwJO69VQ>? NEfqc|gyPzF3OEC1OC=>
mouse HT22 cells MWDGeY5kfGmxbjDhd5NigQ>? NX:xSJd{OyCq NFLSWWFP\XW{b4Dyc5Rm[3SrdnWgZYN1cX[rdImgbY4hdW:3c3WgTHQzOiClZXzsd{Bie3Onc4Pl[EBieyC2LVLPU2gucW6mdXPl[EB1d3irY3n0fUBifCB2MDD1UUBxemWrbnP1ZoF1\WRiZn;yJFMhcHK|IH\vcIxwf2WmIHL5JJQuSk:RSDDpcoR2[3Srb36gcYVie3W{ZXSgZYZ1\XJiOTDodpMh[nliTWTUJIF{e2G7 MlLyNlQzPDV7M{m=
human THP1 cells M3;tfGZ2dmO2aX;uJIF{e2G7 MUSyNEDPxE1? NHqyfXgyKGh? MmjNSI94dnKnZ4XsZZRqd25ib3[gWHBCNWmwZIXj[YQhVk:[MjDtVm5CKGW6cILld5Nqd25iaX6gbJVu[W5iVFjQNUBk\WyuczDheEAzOCC3TTDpcoN2[mG2ZXSg[o9zKDFiaIKgdJJqd3JidH:gWHBCKGOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAzPCCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nz MoLINlM4QDZ3MkC=
MDA-MB-231 cells MULGeY5kfGmxbjDhd5NigQ>? MX61JO69VQ>? NX\0S2ZsOTZiaB?= MoezTY5pcWKrdHnvckBw\iCSTVGtd5RqdXWuYYTl[EBPTi2tYYDwZWIhe2mpbnHsbY5oKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOUB2VSCrbnP1ZoF1\WRiZn;yJFE3KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? M3T2e|I2OTlyNE[2
HL60 cells NELENJhRem:uaX\ldoF1cW:wIHHzd4F6 MlPWN|Ah|ryP MVO0PEBp NX;HV2Z7SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIF1KDNyIIXNJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO|YYm= NEHO[oQyQDJ3OES0NC=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FADD / PARP / Cleaved PARP ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of FADD, PARP, and cleaved PARP were then measured by Western blot analysis.

Caspase-3 / Cleaved Caspase-3 / Caspase-8 / Cleaved Caspase-8 ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of Caspase-8, cleaved Caspase-8, Caspase-3, and cleaved Caspase-3 were measured by Western blot analysis. 

ERK / p-ERK / JNK / p-JNK / p38 / p-p38 / Bax / Bcl-2 ; 

PubMed: 30992674     


LN229 cells were treated with luteolin (0, 10, 20, and 30 µM) for 24 hours, and Western blot analysis was performed to measure the expression levels of ERK, P-ERK, JNK, P-JNK, p38, P-p38, Bax, and Bcl-2. 

p-VEGFR2 / p-mTOR / pS6K1 / p70S6K1 / pAKT / AKT / MMP-2 / MMP-9 ; 

PubMed: 23300633     


Luteolin suppressed the activation of VEGFR2 and their downstream signaling pathway triggered by VEGF in HUVECs. Proteins from different treatments was tested by Western blotting and probed with specific antibodies. Experiments were repeated for three times. 

p21 / Survivin / Cyclin D1; 

PubMed: 30930986     


Total protein was isolated from control and luteolin-treated MDA-MB-231 cells, and subjected to immunoblotting of p21, Survivin and cyclin D1 proteins. Membranes were stripped and re-probed with anti-GAPDH antibody to ensure equal protein loading. p21 was upregulated, whereas Survivin and cyclin D1 were downregulated in MDA-MB-231 cell in a dose-dependent manner. *P<0.05 vs. the control group (0 µM luteolin); #P<0.05 vs. the 10 µM luteolin group.

DNMT1 / DMNT3A / DNMT3B ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against DNMT1, DNMT3A, DNMT3B.

TET1 / TET2 / TET3 ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against TET1, TET2, and TET3.

30992674 23300633 30930986 30988303
Immunofluorescence
5-hmC ; 

PubMed: 30988303     


Formation of 5-hmC by TETs was assessed using confocal imaging.

30988303
Growth inhibition assay
Cell viability; 

PubMed: 30992674     


(B) LN229 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (C) U251 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (D) HEB cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay.

30992674
In vivo LD50: Mice >2500mg/kg (i.g.) [3]

Protocol

Solubility (25°C)

In vitro DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL warmed (10.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 286.24
Formula

C15H10O6

CAS No. 491-70-3
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    Would you please suggest a suitable vehicle to dissolve Luteolin for in vivo i.p. use?

  • Answer:

    Formula: 5% DMSO+40% PEG 300+5% Tween80+ddH2O, working Solution concentration: up to 7.5mg/ml, stable for 30min.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID