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Luteolin

For research use only.

Catalog No.S2320

11 publications

Luteolin Chemical Structure

CAS No. 491-70-3

Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.

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Selleck's Luteolin has been cited by 11 publications

1 Customer Review

  • Bioactive compounds inhibit recombinant HDAC activity. Recombinant HDACs were incubated with flavonoid Luteolin (C) for 2 h prior to the addition of cell permeable HDAC substrates. Luteolin inhibited activity of most classes I, IIa, and IIb HDAC isoforms.

    Mol Nutr Food Res, 2017, doi: 10.1002/mnfr.201600744. Luteolin purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.
Targets
PDE2 [1]
(Cell-free assay)
PDE5 [1]
(Cell-free assay)
PDE4 [1]
(Cell-free assay)
PDE3 [1]
(Cell-free assay)
PDE1 [1]
(Cell-free assay)
6.4 μM(Ki) 9.5 μM(Ki) 11.1 μM(Ki) 13.9 μM(Ki) 15.0 μM(Ki)
In vitro

Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. [1] Luteolin inhibits LPS-stimulated TNF-alpha production with an IC50 of less than 1 μM. Luteolin inhibites LPS-induced phosphorylation of Akt as well as IkappaBalpha. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells M2rmN2Z2dmO2aX;uJIF{e2G7 MW[yJIg> M1nscGlvcGmkaYTpc44hd2ZiTl:gdJJw\HWldHnvckBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDSRXczPjRwNzDj[YxteyCycnWtbY5kfWKjdHXkJIZweiB{IHjyd{Bj\W[xcnWgUHBUKHO2aX31cIF1cW:wIH\vdkAzPCCqcoOgZpkhT3KrZYPzJIF{e2G7IH3leIhw\CxiSVO1NF0xNjJzIN88US=> MkHCNlUyPzZzOEe=
HEK293 FS cells NVnpVYZvTnWwY4Tpc44h[XO|YYm= NGfIellKdmirYnn0bY9vKG:oIF7PXFQh\XiycnXzd4VlKGmwIFjFT|I6OyCIUzDj[YxteyCjc4Pld5Nm\CCjczDINm8zKHC{b3T1Z5Rqd25iYomgTFJQOi:WeYKvUHBQKGG|c3H5MEBKSzVyPUCuPFUh|ryP MmHDNlA4OzF|NUe=
CHO cells MYLGeY5kfGmxbjDhd5NigQ>? NIHQU|hC\2:waYP0JIFkfGm4aYT5JIF1KHKjdDDERXQh\XiycnXzd4VlKGmwIFPIU{Bk\WyuczygSWM2OD1zLkS1JO69VQ>? NULJdYF1OjB7N{G2OVA>
human MV4-11 cells MVrDfZRwfG:6aXPpeJkh[XO|YYm= MV:3NkBp NWnOdGNLS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVVZ2LUGxJINmdGy|IHjhdoJwemmwZzDGUHQ{KG23dHH0bY9vKGGodHXyJFczKGi{czDifUB1\XS{YYrvcIl2dSCkYYPl[EBGgiCFeWTvfEBk\WyuII\pZYJqdGm2eTDhd5NigSxiR1m1NF0yNjd4IN88US=> MmTTNlM1OTFyN{O=
HEK293 cells M3v0[WZ2dmO2aX;uJIF{e2G7 MnHVNlQhcA>? NFG5RnBC\2:waYP0JIFkfGm4aYT5JIF1KG2xdYPlJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJINwNWW6cILld5Nqdmdid3n0bEBI[Wx2IILldI9zfGW{II\lZ5RweiCjZoTldkAzPCCqcoOgZpkh\HWjbD3seYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7LDDFR|UxRTJwMzFOwG0> MVWyOFk2PTh6OR?=
human U2OS cells MoPGSpVv[3Srb36gZZN{[Xl? NETSV3c2KGh? NGrW[WxC\2:waYP0JIFkfGm4aYT5JIF1KEeSUkO1JJJm[2WydH;yJIlvKGi3bXHuJHUzV1NiY3XscJMh[2:neIDy[ZN{cW6pIFfhcFQuXlBzNj3USXYh[XO|ZYPz[YQh[XNiYnX0ZUBienKnc4TpckB1emGwc3zvZ4F1cW:wIHHmeIVzKDViaILzJIJ6KGKndHGgcIFkfGGvYYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEWFNUC9N{4zKM7:TR?= NGjvZpozPDlyMES0Oy=>
RBL-2H3 cells MmnYSpVv[3Srb36gZZN{[Xl? M1v2U2lvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBKVC12IIDyc4R2[3Srb36gbY4hWkKOLULIN{Bk\WyuczD3ZZMh\GW2ZYLtbY5m\CxiSVO1NF0{NjdizszN MX:xNlk2OTB7Mh?=
human mast cells M{K0b2Z2dmO2aX;uJIF{e2G7 M2jxRmlvcGmkaYTpc44hd2ZiU2nLJIlvKGi3bXHuJI1ie3RiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJI1ie3RiY3XscEBl\We{YX71cIF1cW:wLDDFR|UxRTRwNTFOwG0> MX6yNlI2PzJzMx?=
human LNCAP cells MV3GeY5kfGmxbjDhd5NigQ>? Mlr2SI94dnKnZ4XsZZRqd25ib3[gdJJwe3SjdHWgd5Bm[2moaXOgZY51cWenbjDz[YNz\XSrb36gbY4hcHWvYX6gUG5ESVBiY3XscJMtKEmFNUC9OUDPxE1? MXmyOVAyQTR5OB?=
rat H9c2 cells NIDw[JVHfW6ldHnvckBie3OjeR?= M17kXFI1KGh? NVG1V41DS3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDkc5hwenWkaXPpck1qdmS3Y3XkJIN6fG:2b4jpZ4l1gSCrbjDyZZQhUDmlMjDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCHQ{WwQVUvPTNizszN NVvKdIVEOjB7M{K3OlI>
human HT-29 cells NYSwbGY4TnWwY4Tpc44h[XO|YYm= MoTiNVAhdWmwcx?= MlfpRYdwdmm|dDDhZ5Rqfmm2eTDheEBIWFJ|NTDy[YNmeHSxcjDpckBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjCxNEBucW6|IHL5JIR6dmGvaXOgcYF{eyC{ZXTpd5RzcWK3dHnvckBie3OjeTygSWM2OD15LkK0JO69VQ>? MYWyOFkxODR2Nx?=
human RS4:11 cells MoGzR5l1d3SxeHnjbZR6KGG|c3H5 M{jN[FczKGh? MlKxR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVnM1QjFzIHPlcIx{KGijcnLvdolv\yC5aXzkJJR6eGViRlzUN{Bi\nSncjC3NkBpenNiYomgeIV1emG8b3zpeY0h[mG|ZXSgSZohS3mWb4igZ4VtdCC4aXHibYxqfHliYYPzZZktKEeLNUC9O{4zPSEQvF2= M4qx[|I{PDFzMEez
NCI-H460 cells MV\GeY5kfGmxbjDhd5NigQ>? M1nqSlIuOjBiaB?= MWXJcohq[mm2aX;uJI9nKEGEQ1eyJIV5eHKnc4Pl[EBqdiCqdX3hckBPS0lvSES2NEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGDoRUBi[2O3bYXsZZRqd25iYX\0[ZIhOiC2bzCyNEBpenNicnXsZZRqfmVidH:g[pVucXS{ZX3vdodqdiCFLDDJR|UxRThwOTFOwG0> NVjIb|NWOjF{N{WzPFY>
human H9 cells NGnXb4NHfW6ldHnvckBie3OjeR?= Mmj0N{Bl[Xm| NUG1OXJsSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTCzRkBqdm[nY4Tl[EBqdiCqdX3hckBJQSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEOg[IF6eyCkeTDwNlQh[W62aXflckBk[XC2dYLlJIF{e2G7LDDFR|UxRTFyIN88US=> M3\5VlgyPThzNkS=
MDCK cells NXTO[ZBZS3m2b4TvfIlkcXS7IHHzd4F6 NHHhcYdEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFPLJINmdGy|IHL5JG1VXCCjc4PhfUwhS0N3ME2xNk41PCEQvF2= Ml7TNVg3PDByNEK=
mouse B16-4A5 cells MoDsSpVv[3Srb36gZZN{[Xl? NIP5VGg4OiCq NVjOeGduUW6qaXLpeIlwdiCxZjD0bIVweGi7bHzpcoUue3SrbYXsZZRm\CCvZXzhco9o\W6nc3nzJIlvKG2xdYPlJGIyPi12QUWgZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUG0JO69VQ>? NX\vdHVyOjBzOEmzPVk>
K562 cells MlfBS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2rEclUh\GG7cx?= NF;uVolIem:5dHigbY5pcWKrdHnvckBw\iCNNU[yJINmdGy|IHL5JHhVXCCjc4PhfUBi\nSncjC1JIRigXNuIFnDOVA:OTRwNkWg{txO Mn3jNVc1OTFyOUK=
human H9 cells NVv4dmJWS3m2b4TvfIlkcXS7IHHzd4F6 NEXrTJQ{KGSjeYO= MYPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIPUBk\WyuczDh[pRmeiB|IHThfZMtKEmFNUC9NVYh|ryP M2PUUFgyPThzNkS=
human HT-29 cells NXnWWZpjTnWwY4Tpc44h[XO|YYm= NXXse5JQOTBibXnudy=> NHPXcW1F\XOnboPpeIl7[XSrb36gc4YhT1CUM{WgdoVk\XC2b4KgbY4hcHWvYX6gTHQuOjliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB7[XC{aX7hd5QucW6mdXPl[EBlgW6jbXnjJI1ie3NicnXkbZN1emmkdYTpc44h[W[2ZYKgNVAhdWmwczygTWM2OD1zOD62JO69VQ>? NYHvc4RIOjR7MEC0OFc>
human U937 cells MUXQdo9tcW[ncnH0bY9vKGG|c3H5 MXq3NkBp M4PtPWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVUmzO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliV2PUMVgh[XO|YYmsJGlEPTB;MkCg{txO NVHHV|ZWOTdzNUiwOVQ>
mouse HT22 cells MkD2SpVv[3Srb36gZZN{[Xl? NVjtVYxXOyCq MVPO[ZVzd3C{b4TlZ5RqfmViYXP0bZZqfHliaX6gcY92e2ViSGSyNkBk\WyuczDhd5Nme3OnZDDhd{B1NUKRT1itbY5lfWOnZDD0c5hq[2m2eTDheEA1OCC3TTDwdoVqdmO3YnH0[YQh\m:{IEOgbJJ{KG[xbHzve4VlKGK7IIStRm9QUCCrbnT1Z5Rqd25ibXXhd5Vz\WRiYX\0[ZIhQSCqcoOgZpkhVVSWIHHzd4F6 MXKyOFI1PTl|OR?=
human THP1 cells NGjB[WpHfW6ldHnvckBie3OjeR?= NWLYOmI{OjBizszN Mom3NUBp M1jpd2Rwf26{ZXf1cIF1cW:wIH;mJHRRSS2rbnT1Z4VlKE6RWEKgcXJPSSCneIDy[ZN{cW:wIHnuJIh2dWGwIGTIVFEh[2WubIOgZZQhOjBidV2gbY5kfWKjdHXkJIZweiBzIHjyJJBzcW:{IITvJHRRSSClaHHscIVv\2VibXXhd5Vz\WRiYX\0[ZIhOjRiaILzJIJ6KFKWLWDDVkBidmGueYPpdy=> M{Tib|I{Pzh4NUKw
MDA-MB-231 cells MmjnSpVv[3Srb36gZZN{[Xl? MknJOUDPxE1? MoXYNVYhcA>? MoT4TY5pcWKrdHnvckBw\iCSTVGtd5RqdXWuYYTl[EBPTi2tYYDwZWIhe2mpbnHsbY5oKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOUB2VSCrbnP1ZoF1\WRiZn;yJFE3KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? MXSyOVE6ODR4Nh?=
HL60 cells Mmj0VJJwdGmoZYLheIlwdiCjc4PhfS=> MUezNEDPxE1? NWj0fVY6PDhiaB?= NVm4[HVMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIF1KDNyIIXNJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO|YYm= NGTRfoQyQDJ3OES0NC=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FADD / PARP / Cleaved PARP ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of FADD, PARP, and cleaved PARP were then measured by Western blot analysis.

Caspase-3 / Cleaved Caspase-3 / Caspase-8 / Cleaved Caspase-8 ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of Caspase-8, cleaved Caspase-8, Caspase-3, and cleaved Caspase-3 were measured by Western blot analysis. 

ERK / p-ERK / JNK / p-JNK / p38 / p-p38 / Bax / Bcl-2 ; 

PubMed: 30992674     


LN229 cells were treated with luteolin (0, 10, 20, and 30 µM) for 24 hours, and Western blot analysis was performed to measure the expression levels of ERK, P-ERK, JNK, P-JNK, p38, P-p38, Bax, and Bcl-2. 

p-VEGFR2 / p-mTOR / pS6K1 / p70S6K1 / pAKT / AKT / MMP-2 / MMP-9 ; 

PubMed: 23300633     


Luteolin suppressed the activation of VEGFR2 and their downstream signaling pathway triggered by VEGF in HUVECs. Proteins from different treatments was tested by Western blotting and probed with specific antibodies. Experiments were repeated for three times. 

p21 / Survivin / Cyclin D1; 

PubMed: 30930986     


Total protein was isolated from control and luteolin-treated MDA-MB-231 cells, and subjected to immunoblotting of p21, Survivin and cyclin D1 proteins. Membranes were stripped and re-probed with anti-GAPDH antibody to ensure equal protein loading. p21 was upregulated, whereas Survivin and cyclin D1 were downregulated in MDA-MB-231 cell in a dose-dependent manner. *P<0.05 vs. the control group (0 µM luteolin); #P<0.05 vs. the 10 µM luteolin group.

DNMT1 / DMNT3A / DNMT3B ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against DNMT1, DNMT3A, DNMT3B.

TET1 / TET2 / TET3 ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against TET1, TET2, and TET3.

30992674 23300633 30930986 30988303
Immunofluorescence
5-hmC ; 

PubMed: 30988303     


Formation of 5-hmC by TETs was assessed using confocal imaging.

30988303
Growth inhibition assay
Cell viability; 

PubMed: 30992674     


(B) LN229 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (C) U251 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (D) HEB cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay.

30992674
In vivo LD50: Mice >2500mg/kg (i.g.) [3]

Protocol

Solubility (25°C)

In vitro DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL warmed (10.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 286.24
Formula

C15H10O6

CAS No. 491-70-3
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=C(C=C1C2=CC(=O)C3=C(C=C(C=C3O2)O)O)O)O

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    Would you please suggest a suitable vehicle to dissolve Luteolin for in vivo i.p. use?

  • Answer:

    Formula: 5% DMSO+40% PEG 300+5% Tween80+ddH2O, working Solution concentration: up to 7.5mg/ml, stable for 30min.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID