Luteolin

For research use only.

Catalog No.S2320

10 publications

Luteolin Chemical Structure

CAS No. 491-70-3

Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 70 In stock
USD 50 In stock
USD 170 In stock
USD 570 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Luteolin has been cited by 10 publications

1 Customer Review

  • Bioactive compounds inhibit recombinant HDAC activity. Recombinant HDACs were incubated with flavonoid Luteolin (C) for 2 h prior to the addition of cell permeable HDAC substrates. Luteolin inhibited activity of most classes I, IIa, and IIb HDAC isoforms.

    Mol Nutr Food Res, 2017, doi: 10.1002/mnfr.201600744. Luteolin purchased from Selleck.

Purity & Quality Control

Choose Selective ADC Cytotoxin Inhibitors

Biological Activity

Description Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.
Targets
PDE2 [1]
(Cell-free assay)
PDE5 [1]
(Cell-free assay)
PDE4 [1]
(Cell-free assay)
PDE3 [1]
(Cell-free assay)
PDE1 [1]
(Cell-free assay)
6.4 μM(Ki) 9.5 μM(Ki) 11.1 μM(Ki) 13.9 μM(Ki) 15.0 μM(Ki)
In vitro

Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. [1] Luteolin inhibits LPS-stimulated TNF-alpha production with an IC50 of less than 1 μM. Luteolin inhibites LPS-induced phosphorylation of Akt as well as IkappaBalpha. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NELVSpRHfW6ldHnvckBie3OjeR?= MkjvNkBp NEDUOFJKdmirYnn0bY9vKG:oIF7PJJBzd2S3Y4Tpc44hcW5iTGDTMZN1cW23bHH0[YQhdW:3c3WgVmFYOjZ2LkegZ4VtdHNicILlMYlv[3WkYYTl[EBnd3JiMjDodpMh[mWob4LlJGxRWyC|dHnteYxifGmxbjDmc5IhOjRiaILzJIJ6KEe{aXXzd{Bie3OjeTDt[ZRpd2RuIFnDOVA:OC5{MTFOwG0> MX:yOVE4PjF6Nx?=
HEK293 FS cells NHLPVm5HfW6ldHnvckBie3OjeR?= Mn\4TY5pcWKrdHnvckBw\iCQT2i0JIV5eHKnc4Pl[EBqdiCKRVuyPVMhTlNiY3XscJMh[XO|ZYPz[YQh[XNiSELPNkBxem:mdXP0bY9vKGK7IFiyU|IwXHm{L1zQU{Bie3OjeTygTWM2OD1yLki1JO69VQ>? M2Ln[|IxPzNzM{W3
CHO cells M4GxSmZ2dmO2aX;uJIF{e2G7 MljpRYdwdmm|dDDhZ5Rqfmm2eTDheEBz[XRiRFHUJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFXDOVA:OS52NTFOwG0> MmLtNlA6PzF4NUC=
human MV4-11 cells MnXwR5l1d3SxeHnjbZR6KGG|c3H5 NGTKVYw4OiCq MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNWlQuOTFiY3XscJMhcGG{Yn;ybY5oKE[OVEOgcZV1[XSrb36gZYZ1\XJiN{KgbJJ{KGK7IITleJJigm:uaYXtJIJie2WmIFX6JGN6XG:6IHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBIUTVyPUGuO|Yh|ryP NWnJVJpqOjN2MUGwO|M>
HEK293 cells M3S2SWZ2dmO2aX;uJIF{e2G7 NXi5OG15OjRiaB?= NIHNflRC\2:waYP0JIFkfGm4aYT5JIF1KG2xdYPlJHBRSVKpYX3tZUBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJINwNWW6cILld5Nqdmdid3n0bEBI[Wx2IILldI9zfGW{II\lZ5RweiCjZoTldkAzPCCqcoOgZpkh\HWjbD3seYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7LDDFR|UxRTJwMzFOwG0> M2D2elI1QTV3OEi5
human U2OS cells M1PQ[GZ2dmO2aX;uJIF{e2G7 M1jvUVUhcA>? MlL1RYdwdmm|dDDhZ5Rqfmm2eTDheEBIWFJ|NTDy[YNmeHSxcjDpckBpfW2jbjDVNm9UKGOnbHzzJINw\XiycnXzd4lv\yCJYXy0MXZROTZvVFXWJIF{e2W|c3XkJIF{KGKndHGgZZJz\XO2aX6geJJidnOub3PheIlwdiCjZoTldkA2KGi{czDifUBj\XSjIHzhZ5RidWG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBGSzVyPUOuNkDPxE1? MVKyOFkxODR2Nx?=
RBL-2H3 cells M3:xPGZ2dmO2aX;uJIF{e2G7 MnnhTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGlNNTRicILv[JVkfGmxbjDpckBTSkxvMlizJINmdGy|IIfhd{Bl\XSncn3pcoVlNCCLQ{WwQVMvPyEQvF2= MYSxNlk2OTB7Mh?=
human mast cells M2LNeGZ2dmO2aX;uJIF{e2G7 NUL6eGFJUW6qaXLpeIlwdiCxZjDTXWshcW5iaIXtZY4hdWG|dDDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hdWG|dDDj[YxtKGSnZ4LhcpVt[XSrb36sJGVEPTB;ND61JO69VQ>? M2rXR|IzOjV5MkGz
human LNCAP cells NX7wWZRxTnWwY4Tpc44h[XO|YYm= NITOR2ZFd3ewcnXneYxifGmxbjDv[kBxem:|dHH0[UB{eGWlaX\pZ{BidnSrZ3XuJJNm[3KndHnvckBqdiCqdX3hckBNVkODUDDj[YxteyxiSVO1NF02KM7:TR?= MYCyOVAyQTR5OB?=
rat H9c2 cells MmnhSpVv[3Srb36gZZN{[Xl? NYrwS25XOjRiaB?= Ml7xR5l1d3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCmb4jvdpVjcWOrbj3pcoR2[2WmIHP5eI91d3irY3n0fUBqdiC{YYSgTFlkOiClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBGSzVyPUWuOVMh|ryP M3rOdVIxQTN{N{[y
human HT-29 cells NXW0OXVQTnWwY4Tpc44h[XO|YYm= MkPINVAhdWmwcx?= M2j0fGFod26rc4SgZYN1cX[rdImgZZQhT1CUM{WgdoVk\XC2b4KgbY4hcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgNVAhdWmwczDifUBlgW6jbXnjJI1ie3NicnXkbZN1emmkdYTpc44h[XO|YYmsJGVEPTB;Nz6yOEDPxE1? NHzNfoQzPDlyMES0Oy=>
human RS4:11 cells NXThPZNSS3m2b4TvfIlkcXS7IHHzd4F6 NUDLb2o6PzJiaB?= M2HhbGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHJUPDpzMTDj[YxteyCqYYLic5Jqdmdid3ns[EB1gXCnIF\MWFMh[W[2ZYKgO|IhcHK|IHL5JJRmfHKjen;sbZVuKGKjc3XkJGV7KEO7VH;4JINmdGxidnnhZoltcXS7IHHzd4F6NCCJSUWwQVcvOjVizszN MYqyN|QyOTB5Mx?=
NCI-H460 cells NIT5VG9HfW6ldHnvckBie3OjeR?= NVflUVZyOi1{MDDo NYPScnp7UW6qaXLpeIlwdiCxZjDBRmNIOiCneIDy[ZN{\WRiaX6gbJVu[W5iTlPJMWg1PjBiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBRcEFiYXPjeY12dGG2aX;uJIFnfGW{IEKgeI8hOjBiaILzJJJmdGG2aY\lJJRwKG[3bXn0doVud3KpaX6gR{whUUN3ME24Mlkh|ryP M3\QOlIyOjd3M{i2
human H9 cells NHnSSoFHfW6ldHnvckBie3OjeR?= NFrnfXg{KGSjeYO= NIPjSnRCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKGi3bXHuJGg6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IICyOEBidnSrZ3XuJINieHS3cnWgZZN{[XluIFXDOVA:OTBizszN M4rIVFgyPThzNkS=
MDCK cells MXLDfZRwfG:6aXPpeJkh[XO|YYm= Mn;jR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSFSzDj[YxteyCkeTDNWHQh[XO|YYmsJGNEPTB;MUKuOFQh|ryP NGTVfJUyQDZ2MEC0Ni=>
mouse B16-4A5 cells NF7rc5FHfW6ldHnvckBie3OjeR?= NWLXZ5UyPzJiaB?= MofFTY5pcWKrdHnvckBw\iC2aHXvdIh6dGyrbnWtd5RqdXWuYYTl[EBu\Wyjbn;n[Y5me2m|IHnuJI1wfXOnIFKxOk01STViY3XscJMh[W[2ZYKgO|IhcHK|LDDJR|UxRTF2IN88US=> MYmyNFE5QTN7OR?=
K562 cells NX;UPZFDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFH4VHU2KGSjeYO= NWXn[ZlQT3Kxd4ToJIlvcGmkaYTpc44hd2ZiS{W2NkBk\WyuczDifUBZXFRiYYPzZZkh[W[2ZYKgOUBl[Xm|LDDJR|UxRTF2Lk[1JO69VQ>? NH7rWHUyPzRzMUC5Ni=>
human H9 cells NVe3T4NCS3m2b4TvfIlkcXS7IHHzd4F6 M3vZU|Mh\GG7cx?= M3i0NGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGg6KGOnbHzzJIFnfGW{IEOg[IF6eyxiSVO1NF0yPiEQvF2= M2j0OVgyPThzNkS=
human HT-29 cells NHvLOpJHfW6ldHnvckBie3OjeR?= MUWxNEBucW6| NWX1OHRoTGW|ZX7zbZRqgmG2aX;uJI9nKEeSUkO1JJJm[2WydH;yJIlvKGi3bXHuJGhVNTJ7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhgmGycnnuZZN1NWmwZIXj[YQh\HmwYX3pZ{Bu[XO|IILl[Il{fHKrYoX0bY9vKGGodHXyJFExKG2rboOsJGlEPTB;MUiuOkDPxE1? NVWwdo9wOjR7MEC0OFc>
human U937 cells NHHzd3hRem:uaX\ldoF1cW:wIHHzd4F6 NVf0OplmPzJiaB?= MoS5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCXOUO3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCZU2StPEBie3OjeTygTWM2OD1{MDFOwG0> MmPlNVcyPThyNUS=
mouse HT22 cells MnXYSpVv[3Srb36gZZN{[Xl? MYCzJIg> MoHPUoV2em:ycn;0[YN1cX[nIHHjeIl3cXS7IHnuJI1wfXOnIFjUNlIh[2WubIOgZZN{\XO|ZXSgZZMhfC2ET1;IMYlv\HWlZXSgeI95cWOrdImgZZQhPDBidV2gdJJmcW6ldXLheIVlKG[xcjCzJIhzeyCob3zsc5dm\CCkeTD0MWJQV0hiaX7keYN1cW:wIH3lZZN2emWmIHHmeIVzKDliaILzJIJ6KE2WVDDhd5NigQ>? M{Xj[VI1OjR3OUO5
human THP1 cells MUfGeY5kfGmxbjDhd5NigQ>? NFzzWoQzOCEQvF2= NYX6dGhwOSCq NGXue4tFd3ewcnXneYxifGmxbjDv[kBVWEFvaX7keYNm\CCQT2iyJI1TVkFiZYjwdoV{e2mxbjDpckBpfW2jbjDUTHAyKGOnbHzzJIF1KDJyIIXNJIlv[3WkYYTl[EBnd3JiMTDodkBxemmxcjD0c{BVWEFiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZM> NGD6[ogzOzd6NkWyNC=>
MDA-MB-231 cells MnnxSpVv[3Srb36gZZN{[Xl? NXvGOotrPSEQvF2= NUnJZmtEOTZiaB?= NFu5[|VKdmirYnn0bY9vKG:oIGDNRU1{fGmvdXzheIVlKE6ILXvhdJBiSiC|aXfuZYxqdmdiKIXub45wf25ib4Lp[4lvMSCneIDy[ZN{\WRiaX6gUWRCNU2ELUKzNUBk\WyuczDheEA2KHWPIHnuZ5Vj[XSnZDDmc5IhOTZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeR?= M2DFXVI2OTlyNE[2
HL60 cells M3j2V3Bzd2yrZnXyZZRqd25iYYPzZZk> NHLuRXc{OCEQvF2= MVe0PEBp NFnVXmVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjMOlAh[2WubIOgZZQhOzBidV2gZYZ1\XJiNEigbJJ{KGK7IF3UV{Bie3OjeR?= MY[xPFI2QDR2MB?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FADD / PARP / Cleaved PARP ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of FADD, PARP, and cleaved PARP were then measured by Western blot analysis.

Caspase-3 / Cleaved Caspase-3 / Caspase-8 / Cleaved Caspase-8 ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of Caspase-8, cleaved Caspase-8, Caspase-3, and cleaved Caspase-3 were measured by Western blot analysis. 

ERK / p-ERK / JNK / p-JNK / p38 / p-p38 / Bax / Bcl-2 ; 

PubMed: 30992674     


LN229 cells were treated with luteolin (0, 10, 20, and 30 µM) for 24 hours, and Western blot analysis was performed to measure the expression levels of ERK, P-ERK, JNK, P-JNK, p38, P-p38, Bax, and Bcl-2. 

p-VEGFR2 / p-mTOR / pS6K1 / p70S6K1 / pAKT / AKT / MMP-2 / MMP-9 ; 

PubMed: 23300633     


Luteolin suppressed the activation of VEGFR2 and their downstream signaling pathway triggered by VEGF in HUVECs. Proteins from different treatments was tested by Western blotting and probed with specific antibodies. Experiments were repeated for three times. 

p21 / Survivin / Cyclin D1; 

PubMed: 30930986     


Total protein was isolated from control and luteolin-treated MDA-MB-231 cells, and subjected to immunoblotting of p21, Survivin and cyclin D1 proteins. Membranes were stripped and re-probed with anti-GAPDH antibody to ensure equal protein loading. p21 was upregulated, whereas Survivin and cyclin D1 were downregulated in MDA-MB-231 cell in a dose-dependent manner. *P<0.05 vs. the control group (0 µM luteolin); #P<0.05 vs. the 10 µM luteolin group.

DNMT1 / DMNT3A / DNMT3B ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against DNMT1, DNMT3A, DNMT3B.

TET1 / TET2 / TET3 ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against TET1, TET2, and TET3.

30992674 23300633 30930986 30988303
Immunofluorescence
5-hmC ; 

PubMed: 30988303     


Formation of 5-hmC by TETs was assessed using confocal imaging.

30988303
Growth inhibition assay
Cell viability; 

PubMed: 30992674     


(B) LN229 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (C) U251 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (D) HEB cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay.

30992674
In vivo LD50: Mice >2500mg/kg (i.g.) [3]

Protocol

Solubility (25°C)

In vitro DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL warmed (10.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 286.24
Formula

C15H10O6

CAS No. 491-70-3
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC(=C(C=C1C2=CC(=O)C3=C(C=C(C=C3O2)O)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    Would you please suggest a suitable vehicle to dissolve Luteolin for in vivo i.p. use?

  • Answer:

    Formula: 5% DMSO+40% PEG 300+5% Tween80+ddH2O, working Solution concentration: up to 7.5mg/ml, stable for 30min.

Tags: buy Luteolin | Luteolin supplier | purchase Luteolin | Luteolin cost | Luteolin manufacturer | order Luteolin | Luteolin distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID