Luteolin

For research use only.

Catalog No.S2320 Synonyms: Luteoline, Luteolol, Digitoflavone

12 publications

Luteolin Chemical Structure

CAS No. 491-70-3

Luteolin (Luteoline, Luteolol, Digitoflavone) is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.

Size Price Stock Quantity  
10mM (1mL in DMSO) RMB 582.73 In stock
RMB 630.63 In stock
RMB 1417.78 In stock
RMB 4686.02 In stock
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Selleck's Luteolin has been cited by 12 publications

1 Customer Review

  • Bioactive compounds inhibit recombinant HDAC activity. Recombinant HDACs were incubated with flavonoid Luteolin (C) for 2 h prior to the addition of cell permeable HDAC substrates. Luteolin inhibited activity of most classes I, IIa, and IIb HDAC isoforms.

    Mol Nutr Food Res, 2017, doi: 10.1002/mnfr.201600744. Luteolin purchased from Selleck.

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Biological Activity

Description Luteolin (Luteoline, Luteolol, Digitoflavone) is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.
Targets
PDE2 [1]
(Cell-free assay)
PDE5 [1]
(Cell-free assay)
PDE4 [1]
(Cell-free assay)
PDE3 [1]
(Cell-free assay)
PDE1 [1]
(Cell-free assay)
6.4 μM(Ki) 9.5 μM(Ki) 11.1 μM(Ki) 13.9 μM(Ki) 15.0 μM(Ki)
In vitro

Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. [1] Luteolin inhibits LPS-stimulated TNF-alpha production with an IC50 of less than 1 μM. Luteolin inhibites LPS-induced phosphorylation of Akt as well as IkappaBalpha. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NXv2V5k5TnWwY4Tpc44h[XO|YYm= NEDPZYQzKGh? MmnpTY5pcWKrdHnvckBw\iCQTzDwdo9lfWO2aX;uJIlvKEySUz3zeIlufWyjdHXkJI1wfXOnIGLBW|I3PC55IHPlcIx{KHC{ZT3pcoN2[mG2ZXSg[o9zKDJiaILzJIJm\m:{ZTDMVHMhe3SrbYXsZZRqd25iZn;yJFI1KGi{czDifUBIemmnc4OgZZN{[XlibXX0bI9lNCCLQ{WwQVAvOjFizszN NHvWeHMzPTF5NkG4Oy=>
HEK293 FS cells M{P4UGZ2dmO2aX;uJIF{e2G7 M3ni[mlvcGmkaYTpc44hd2ZiTl;YOEBmgHC{ZYPz[YQhcW5iSFXLNlk{KE[VIHPlcIx{KGG|c3Xzd4VlKGG|IFiyU|IheHKxZIXjeIlwdiCkeTDINm8zN1S7cj;MVG8h[XO|YYmsJGlEPTB;MD64OUDPxE1? NEOweZAzODd|MUO1Oy=>
CHO cells MkHzSpVv[3Srb36gZZN{[Xl? M1PaemFod26rc4SgZYN1cX[rdImgZZQhemG2IFTBWEBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|LDDFR|UxRTFwNEWg{txO NFTSeJMzODl5MU[1NC=>
human MV4-11 cells NIjLNmZEgXSxdH;4bYNqfHliYYPzZZk> NEHne4I4OiCq NHPhW2VEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXjRvMUGgZ4VtdHNiaHHyZo9zcW6pIF\MWFMhdXW2YYTpc44h[W[2ZYKgO|IhcHK|IHL5JJRmfHKjen;sbZVuKGKjc3XkJGV7KEO7VH;4JINmdGxidnnhZoltcXS7IHHzd4F6NCCJSUWwQVEvPzZizszN NHfLemkzOzRzMUC3Ny=>
HEK293 cells NULMbHZJTnWwY4Tpc44h[XO|YYm= NH3EcpgzPCCq M2r0dWFod26rc4SgZYN1cX[rdImgZZQhdW:3c3WgVHBCWmejbX3hJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZ48u\XiycnXzd4lv\yC5aYToJGdidDRicnXwc5J1\XJidnXjeI9zKGGodHXyJFI1KGi{czDifUBlfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKgZZN{[XluIFXDOVA:Oi5|IN88US=> M4nidlI1QTV3OEi5
human U2OS cells M3vVdGZ2dmO2aX;uJIF{e2G7 M4XK[lUhcA>? M3;2b2Fod26rc4SgZYN1cX[rdImgZZQhT1CUM{WgdoVk\XC2b4KgbY4hcHWvYX6gWVJQWyClZXzsd{Bkd2W6cILld5NqdmdiR3HsOE1XWDF4LWTFWkBie3Onc4Pl[EBieyCkZYThJIFzemW|dHnuJJRz[W6|bH;jZZRqd25iYX\0[ZIhPSCqcoOgZpkh[mW2YTDsZYN1[W2jc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhTUN3ME2zMlIh|ryP NULWNpQ4OjR7MEC0OFc>
RBL-2H3 cells M2Hjb2Z2dmO2aX;uJIF{e2G7 M2PBbWlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBKVC12IIDyc4R2[3Srb36gbY4hWkKOLULIN{Bk\WyuczD3ZZMh\GW2ZYLtbY5m\CxiSVO1NF0{NjdizszN NYXHZoNJOTJ7NUGwPVI>
human mast cells NWO0Rm53TnWwY4Tpc44h[XO|YYm= MXjJcohq[mm2aX;uJI9nKFO\SzDpckBpfW2jbjDtZZN1KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDtZZN1KGOnbHyg[IVoemGwdXzheIlwdixiRVO1NF01NjVizszN MWGyNlI2PzJzMx?=
human LNCAP cells MUTGeY5kfGmxbjDhd5NigQ>? M2fYOmRwf26{ZXf1cIF1cW:wIH;mJJBzd3O2YYTlJJNx\WOrZnnjJIFvfGmpZX6gd4VkemW2aX;uJIlvKGi3bXHuJGxPS0GSIHPlcIx{NCCLQ{WwQVUh|ryP NFjLTnAzPTBzOUS3PC=>
rat H9c2 cells NUTVU3hQTnWwY4Tpc44h[XO|YYm= Mnv5NlQhcA>? NETXUpJEgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTvfI9zfWKrY3nuMYlv\HWlZXSgZ5l1d3SxeHnjbZR6KGmwIILheEBJQWN{IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEWFNUC9OU42OyEQvF2= NGCzUFgzODl|Mke2Ni=>
human HT-29 cells NFfTfVFHfW6ldHnvckBie3OjeR?= NIqwOoEyOCCvaX7z M3voWGFod26rc4SgZYN1cX[rdImgZZQhT1CUM{WgdoVk\XC2b4KgbY4hcHWvYX6gTHQuOjliY3XscJMh[W[2ZYKgNVAhdWmwczDifUBlgW6jbXnjJI1ie3NicnXkbZN1emmkdYTpc44h[XO|YYmsJGVEPTB;Nz6yOEDPxE1? NVW5V3h5OjR7MEC0OFc>
human RS4:11 cells M4TDUWN6fG:2b4jpZ4l1gSCjc4PhfS=> M{izZlczKGh? MnHyR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVnM1QjFzIHPlcIx{KGijcnLvdolv\yC5aXzkJJR6eGViRlzUN{Bi\nSncjC3NkBpenNiYomgeIV1emG8b3zpeY0h[mG|ZXSgSZohS3mWb4igZ4VtdCC4aXHibYxqfHliYYPzZZktKEeLNUC9O{4zPSEQvF2= NH\MN5gzOzRzMUC3Ny=>
NCI-H460 cells NYnNfWFrTnWwY4Tpc44h[XO|YYm= NULHe3lSOi1{MDDo M{nLdWlvcGmkaYTpc44hd2ZiQVLDS|Ih\XiycnXzd4VlKGmwIHj1cYFvKE6FST3IOFYxKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gVIhCKGGlY4XteYxifGmxbjDh[pRmeiB{IITvJFIxKGi{czDy[YxifGm4ZTD0c{BnfW2rdILlcY9z\2mwIFOsJGlEPTB;OD65JO69VQ>? MkHkNlEzPzV|OE[=
human H9 cells NEPlV|NHfW6ldHnvckBie3OjeR?= NYP4XGo5OyCmYYnz NYrtc5VNSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEmYMTCzRkBqdm[nY4Tl[EBqdiCqdX3hckBJQSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIILldIxq[2G2aX;uJIFnfGW{IEOg[IF6eyCkeTDwNlQh[W62aXflckBk[XC2dYLlJIF{e2G7LDDFR|UxRTFyIN88US=> MmHiPFE2QDF4NB?=
MDCK cells NYXUV|duS3m2b4TvfIlkcXS7IHHzd4F6 NW\sS|VmS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTDT{Bk\WyuczDifUBOXFRiYYPzZZktKEOFNUC9NVIvPDRizszN M1PtNVE5PjRyMESy
mouse B16-4A5 cells MYnGeY5kfGmxbjDhd5NigQ>? NIPmOZM4OiCq MWjJcohq[mm2aX;uJI9nKHSqZX;wbJltdGmwZT3zeIlufWyjdHXkJI1mdGGwb3flcoV{cXNiaX6gcY92e2ViQkG2MVRCPSClZXzsd{Bi\nSncjC3NkBpenNuIFnDOVA:OTRizszN NIT2[pUzODF6OUO5PS=>
K562 cells NFSybJZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4TSRVUh\GG7cx?= MoHsS5Jwf3SqIHnubIljcXSrb36gc4YhUzV4MjDj[YxteyCkeTDYWHQh[XO|YYmgZYZ1\XJiNTDkZZl{NCCLQ{WwQVE1NjZ3IN88US=> MlPKNVc1OTFyOUK=
human H9 cells NGj1UYhEgXSxdH;4bYNqfHliYYPzZZk> M2fubFMh\GG7cx?= NH[4ZY1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJQSClZXzsd{Bi\nSncjCzJIRigXNuIFnDOVA:OTZizszN MX24NVU5OTZ2
human HT-29 cells NGrIOWxHfW6ldHnvckBie3OjeR?= MojqNVAhdWmwcx?= NGPtVnlF\XOnboPpeIl7[XSrb36gc4YhT1CUM{WgdoVk\XC2b4KgbY4hcHWvYX6gTHQuOjliY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB7[XC{aX7hd5QucW6mdXPl[EBlgW6jbXnjJI1ie3NicnXkbZN1emmkdYTpc44h[W[2ZYKgNVAhdWmwczygTWM2OD1zOD62JO69VQ>? Mn75NlQ6ODB2NEe=
human U937 cells MlP6VJJwdGmoZYLheIlwdiCjc4PhfS=> MYW3NkBp NX34fZhSSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPVM4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDXV3QuQCCjc4PhfUwhUUN3ME2yNEDPxE1? MXmxO|E2QDB3NB?=
mouse HT22 cells NI\hOYxHfW6ldHnvckBie3OjeR?= MUCzJIg> NUXTeZdsVmW3cn;wdo91\WO2aY\lJIFkfGm4aYT5JIlvKG2xdYPlJGhVOjJiY3XscJMh[XO|ZYPz[YQh[XNidD3CU29JNWmwZIXj[YQhfG:6aXPpeJkh[XRiNECgeW0heHKnaX7jeYJifGWmIH\vdkA{KGi{czDmc4xtd3enZDDifUB1NUKRT1igbY5lfWO2aX;uJI1m[XO3cnXkJIFnfGW{IEmgbJJ{KGK7IF3UWEBie3OjeR?= Mn;BNlQzPDV7M{m=
human THP1 cells NX20[ohNTnWwY4Tpc44h[XO|YYm= NX3aWJk2OjBizszN NEWwWWIyKGh? Ml\HSI94dnKnZ4XsZZRqd25ib3[gWHBCNWmwZIXj[YQhVk:[MjDtVm5CKGW6cILld5Nqd25iaX6gbJVu[W5iVFjQNUBk\WyuczDheEAzOCC3TTDpcoN2[mG2ZXSg[o9zKDFiaIKgdJJqd3JidH:gWHBCKGOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAzPCCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nz MknvNlM4QDZ3MkC=
MDA-MB-231 cells NVqxWZp2TnWwY4Tpc44h[XO|YYm= NHvWNY42KM7:TR?= M4TENFE3KGh? MmXjTY5pcWKrdHnvckBw\iCSTVGtd5RqdXWuYYTl[EBPTi2tYYDwZWIhe2mpbnHsbY5oKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOUB2VSCrbnP1ZoF1\WRiZn;yJFE3KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? MVyyOVE6ODR4Nh?=
HL60 cells NGfDbllRem:uaX\ldoF1cW:wIHHzd4F6 NYi2V2dlOzBizszN MonwOFghcA>? NYG4b5dvSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIF1KDNyIIXNJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO|YYm= NX:5XIFSOTh{NUi0OFA>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FADD / PARP / Cleaved PARP ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of FADD, PARP, and cleaved PARP were then measured by Western blot analysis.

Caspase-3 / Cleaved Caspase-3 / Caspase-8 / Cleaved Caspase-8 ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of Caspase-8, cleaved Caspase-8, Caspase-3, and cleaved Caspase-3 were measured by Western blot analysis. 

ERK / p-ERK / JNK / p-JNK / p38 / p-p38 / Bax / Bcl-2 ; 

PubMed: 30992674     


LN229 cells were treated with luteolin (0, 10, 20, and 30 µM) for 24 hours, and Western blot analysis was performed to measure the expression levels of ERK, P-ERK, JNK, P-JNK, p38, P-p38, Bax, and Bcl-2. 

p-VEGFR2 / p-mTOR / pS6K1 / p70S6K1 / pAKT / AKT / MMP-2 / MMP-9 ; 

PubMed: 23300633     


Luteolin suppressed the activation of VEGFR2 and their downstream signaling pathway triggered by VEGF in HUVECs. Proteins from different treatments was tested by Western blotting and probed with specific antibodies. Experiments were repeated for three times. 

p21 / Survivin / Cyclin D1; 

PubMed: 30930986     


Total protein was isolated from control and luteolin-treated MDA-MB-231 cells, and subjected to immunoblotting of p21, Survivin and cyclin D1 proteins. Membranes were stripped and re-probed with anti-GAPDH antibody to ensure equal protein loading. p21 was upregulated, whereas Survivin and cyclin D1 were downregulated in MDA-MB-231 cell in a dose-dependent manner. *P<0.05 vs. the control group (0 µM luteolin); #P<0.05 vs. the 10 µM luteolin group.

DNMT1 / DMNT3A / DNMT3B ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against DNMT1, DNMT3A, DNMT3B.

TET1 / TET2 / TET3 ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against TET1, TET2, and TET3.

30992674 23300633 30930986 30988303
Immunofluorescence
5-hmC ; 

PubMed: 30988303     


Formation of 5-hmC by TETs was assessed using confocal imaging.

30988303
Growth inhibition assay
Cell viability; 

PubMed: 30992674     


(B) LN229 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (C) U251 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (D) HEB cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay.

30992674
In vivo LD50: Mice >2500mg/kg (i.g.) [3]

Protocol

Solubility (25°C)

In vitro DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL warmed (10.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 286.24
Formula

C15H10O6

CAS No. 491-70-3
Storage powder
in solvent
Synonyms Luteoline, Luteolol, Digitoflavone
Smiles C1=CC(=C(C=C1C2=CC(=O)C3=C(C=C(C=C3O2)O)O)O)O

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    Would you please suggest a suitable vehicle to dissolve Luteolin for in vivo i.p. use?

  • Answer:

    Formula: 5% DMSO+40% PEG 300+5% Tween80+ddH2O, working Solution concentration: up to 7.5mg/ml, stable for 30min.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID