Luteolin

For research use only.

Catalog No.S2320 Synonyms: Luteoline, Luteolol, Digitoflavone

14 publications

Luteolin Chemical Structure

CAS No. 491-70-3

Luteolin (Luteoline, Luteolol, Digitoflavone) is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.

Selleck's Luteolin has been cited by 14 publications

1 Customer Review

  • Bioactive compounds inhibit recombinant HDAC activity. Recombinant HDACs were incubated with flavonoid Luteolin (C) for 2 h prior to the addition of cell permeable HDAC substrates. Luteolin inhibited activity of most classes I, IIa, and IIb HDAC isoforms.

    Mol Nutr Food Res, 2017, doi: 10.1002/mnfr.201600744. Luteolin purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Luteolin (Luteoline, Luteolol, Digitoflavone) is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. Phase 2.
Targets
PDE2 [1]
(Cell-free assay)
PDE5 [1]
(Cell-free assay)
PDE4 [1]
(Cell-free assay)
PDE3 [1]
(Cell-free assay)
PDE1 [1]
(Cell-free assay)
6.4 μM(Ki) 9.5 μM(Ki) 11.1 μM(Ki) 13.9 μM(Ki) 15.0 μM(Ki)
In vitro

Luteolin is a flavonoid found in Terminalia chebula, which is a non-selective phisphodiesterase PDE inhibitor for PDE1-5 with Ki of 15.0 μM, 6.4 μM, 13.9 μM, 11.1 μM and 9.5 μM, respectively. [1] Luteolin inhibits LPS-stimulated TNF-alpha production with an IC50 of less than 1 μM. Luteolin inhibites LPS-induced phosphorylation of Akt as well as IkappaBalpha. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NGDCfodHfW6ldHnvckBie3OjeR?= M2TONlIhcA>? M2HTcWlvcGmkaYTpc44hd2ZiTl:gdJJw\HWldHnvckBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDSRXczPjRwNzDj[YxteyCycnWtbY5kfWKjdHXkJIZweiB{IHjyd{Bj\W[xcnWgUHBUKHO2aX31cIF1cW:wIH\vdkAzPCCqcoOgZpkhT3KrZYPzJIF{e2G7IH3leIhw\CxiSVO1NF0xNjJzIN88US=> M2XCW|I2OTd4MUi3
HEK293 FS cells MVrGeY5kfGmxbjDhd5NigQ>? MW\Jcohq[mm2aX;uJI9nKE6RWESg[ZhxemW|c3XkJIlvKEiHS{K5N{BHWyClZXzsd{Bie3Onc4Pl[EBieyCKMl:yJJBzd2S3Y4Tpc44h[nliSELPNk9VgXJxTGDPJIF{e2G7LDDJR|UxRTBwOEWg{txO MYGyNFc{OTN3Nx?=
CHO cells M3jNb2Z2dmO2aX;uJIF{e2G7 MnO3RYdwdmm|dDDhZ5Rqfmm2eTDheEBz[XRiRFHUJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFXDOVA:OS52NTFOwG0> NVrQVZJGOjB7N{G2OVA>
human MV4-11 cells MkfUR5l1d3SxeHnjbZR6KGG|c3H5 MWq3NkBp NI\sVoVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXjRvMUGgZ4VtdHNiaHHyZo9zcW6pIF\MWFMhdXW2YYTpc44h[W[2ZYKgO|IhcHK|IHL5JJRmfHKjen;sbZVuKGKjc3XkJGV7KEO7VH;4JINmdGxidnnhZoltcXS7IHHzd4F6NCCJSUWwQVEvPzZizszN MkO3NlM1OTFyN{O=
HEK293 cells MWTGeY5kfGmxbjDhd5NigQ>? M{jENFI1KGh? Mn3TRYdwdmm|dDDhZ5Rqfmm2eTDheEBud3W|ZTDQVGFT\2GvbXGg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDjc{1mgHC{ZYPzbY5oKHerdHigS4FtPCC{ZYDvdpRmeiC4ZXP0c5Ih[W[2ZYKgNlQhcHK|IHL5JIR2[WxvbIXjbYZmemG|ZTDy[ZBwenSncjDhd5NigSxiRVO1NF0zNjNizszN M{HHfVI1QTV3OEi5
human U2OS cells MYLGeY5kfGmxbjDhd5NigQ>? NUPsZmNJPSCq NYLpdJp4SWexbnnzeEBi[3Srdnn0fUBifCCJUGKzOUBz\WOncITvdkBqdiCqdX3hckBWOk:VIHPlcIx{KGOxZYjwdoV{e2mwZzDHZYw1NV[SMU[tWGVXKGG|c3Xzd4VlKGG|IHLleIEh[XK{ZYP0bY4hfHKjboPsc4NifGmxbjDh[pRmeiB3IHjyd{BjgSCkZYThJIxi[3SjbXHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwQVMvOiEQvF2= Mmi2NlQ6ODB2NEe=
RBL-2H3 cells MXvGeY5kfGmxbjDhd5NigQ>? NGLZPWVKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUUxvNDDwdo9lfWO2aX;uJIlvKFKETD2yTFMh[2WubIOge4F{KGSndHXycYlv\WRuIFnDOVA:Oy55IN88US=> NH7mXokyOjl3MUC5Ni=>
human mast cells NIXEeJZHfW6ldHnvckBie3OjeR?= MX3Jcohq[mm2aX;uJI9nKFO\SzDpckBpfW2jbjDtZZN1KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDtZZN1KGOnbHyg[IVoemGwdXzheIlwdixiRVO1NF01NjVizszN NVrXPGtnOjJ{NUeyNVM>
human LNCAP cells M4fn[mZ2dmO2aX;uJIF{e2G7 MnTXSI94dnKnZ4XsZZRqd25ib3[gdJJwe3SjdHWgd5Bm[2moaXOgZY51cWenbjDz[YNz\XSrb36gbY4hcHWvYX6gUG5ESVBiY3XscJMtKEmFNUC9OUDPxE1? NVfCb2RQOjVyMUm0O|g>
rat H9c2 cells MVfGeY5kfGmxbjDhd5NigQ>? MkH3NlQhcA>? NYC1SHQxS3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDkc5hwenWkaXPpck1qdmS3Y3XkJIN6fG:2b4jpZ4l1gSCrbjDyZZQhUDmlMjDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCHQ{WwQVUvPTNizszN MmLWNlA6OzJ5NkK=
human HT-29 cells M4TBdmZ2dmO2aX;uJIF{e2G7 MXixNEBucW6| NF;lSZZC\2:waYP0JIFkfGm4aYT5JIF1KEeSUkO1JJJm[2WydH;yJIlvKGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFExKG2rboOgZpkh\HmwYX3pZ{Bu[XO|IILl[Il{fHKrYoX0bY9vKGG|c3H5MEBGSzVyPUeuNlQh|ryP NYDxelhSOjR7MEC0OFc>
human RS4:11 cells NHfE[ZVEgXSxdH;4bYNqfHliYYPzZZk> NGTtdJc4OiCq M2X3[2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHJUPDpzMTDj[YxteyCqYYLic5Jqdmdid3ns[EB1gXCnIF\MWFMh[W[2ZYKgO|IhcHK|IHL5JJRmfHKjen;sbZVuKGKjc3XkJGV7KEO7VH;4JINmdGxidnnhZoltcXS7IHHzd4F6NCCJSUWwQVcvOjVizszN MofSNlM1OTFyN{O=
NCI-H460 cells NV7qZY5LTnWwY4Tpc44h[XO|YYm= MYeyMVIxKGh? Mk\TTY5pcWKrdHnvckBw\iCDQlPHNkBmgHC{ZYPz[YQhcW5iaIXtZY4hVkOLLVi0OlAh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDQbGEh[WOldX31cIF1cW:wIHHmeIVzKDJidH:gNlAhcHK|IILlcIF1cX[nIITvJIZ2dWm2cnXtc5JocW5iQzygTWM2OD16Lkmg{txO M3XXUFIyOjd3M{i2
human H9 cells NIfTbnBHfW6ldHnvckBie3OjeR?= NH\XXYc{KGSjeYO= NILzfnNCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKGi3bXHuJGg6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IICyOEBidnSrZ3XuJINieHS3cnWgZZN{[XluIFXDOVA:OTBizszN MV24NVU5OTZ2
MDCK cells M1LNXmN6fG:2b4jpZ4l1gSCjc4PhfS=> NEHNemdEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFPLJINmdGy|IHL5JG1VXCCjc4PhfUwhS0N3ME2xNk41PCEQvF2= NXHqUI8xOTh4NECwOFI>
mouse B16-4A5 cells NEnub4pHfW6ldHnvckBie3OjeR?= M2\IbVczKGh? M1PyO2lvcGmkaYTpc44hd2ZidHjlc5BpgWyuaX7lMZN1cW23bHH0[YQhdWWuYX7v[4Vv\XOrczDpckBud3W|ZTDCNVYuPEF3IHPlcIx{KGGodHXyJFczKGi{czygTWM2OD1zNDFOwG0> MYOyNFE5QTN7OR?=
K562 cells NUXT[nRUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWrFR21tPSCmYYnz MX3Hdo94fGhiaX7obYJqfGmxbjDv[kBMPTZ{IHPlcIx{KGK7IGjUWEBie3OjeTDh[pRmeiB3IHThfZMtKEmFNUC9NVQvPjVizszN MW[xO|QyOTB7Mh?=
human H9 cells Moq2R5l1d3SxeHnjbZR6KGG|c3H5 MVezJIRigXN? MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIPUBk\WyuczDh[pRmeiB|IHThfZMtKEmFNUC9NVYh|ryP NHnxflY5OTV6MU[0
human HT-29 cells MVXGeY5kfGmxbjDhd5NigQ>? NFixeXQyOCCvaX7z MlX6SIV{\W6|aYTpfoF1cW:wIH;mJGdRWjN3IILlZ4VxfG:{IHnuJIh2dWGwIFjUMVI6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gfoFxemmwYYP0MYlv\HWlZXSg[Jlv[W2rYzDtZZN{KHKnZHnzeJJq[nW2aX;uJIFnfGW{IEGwJI1qdnNuIFnDOVA:OThwNjFOwG0> NYmxXHl7OjR7MEC0OFc>
human U937 cells MWLQdo9tcW[ncnH0bY9vKGG|c3H5 NXHYWo1WPzJiaB?= NI\XfmdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGW5N|ch[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IGfTWE05KGG|c3H5MEBKSzVyPUKwJO69VQ>? NXrDVpUyOTdzNUiwOVQ>
mouse HT22 cells NYG3XohqTnWwY4Tpc44h[XO|YYm= MlH6N{Bp NFu2epJP\XW{b4Dyc5Rm[3SrdnWgZYN1cX[rdImgbY4hdW:3c3WgTHQzOiClZXzsd{Bie3Onc4Pl[EBieyC2LVLPU2gucW6mdXPl[EB1d3irY3n0fUBifCB2MDD1UUBxemWrbnP1ZoF1\WRiZn;yJFMhcHK|IH\vcIxwf2WmIHL5JJQuSk:RSDDpcoR2[3Srb36gcYVie3W{ZXSgZYZ1\XJiOTDodpMh[nliTWTUJIF{e2G7 MWiyOFI1PTl|OR?=
human THP1 cells MVPGeY5kfGmxbjDhd5NigQ>? NHm1R2YzOCEQvF2= MmjINUBp NFLLO5FFd3ewcnXneYxifGmxbjDv[kBVWEFvaX7keYNm\CCQT2iyJI1TVkFiZYjwdoV{e2mxbjDpckBpfW2jbjDUTHAyKGOnbHzzJIF1KDJyIIXNJIlv[3WkYYTl[EBnd3JiMTDodkBxemmxcjD0c{BVWEFiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZM> NE\2fGQzOzd6NkWyNC=>
MDA-MB-231 cells M4H5emZ2dmO2aX;uJIF{e2G7 NHS2WIk2KM7:TR?= M3rLZVE3KGh? NV\lfHR5UW6qaXLpeIlwdiCxZjDQUWEue3SrbYXsZZRm\CCQRj3rZZBx[UJic3nncoFtcW6pIDj1cotvd3ewIH;ybYdqdiliZYjwdoV{e2WmIHnuJG1FSS2PQj2yN|Eh[2WubIOgZZQhPSC3TTDpcoN2[mG2ZXSg[o9zKDF4IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= MkXlNlUyQTB2Nk[=
HL60 cells NITMe3BRem:uaX\ldoF1cW:wIHHzd4F6 MlLDN|Ah|ryP MmTQOFghcA>? NFnuOFFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjMOlAh[2WubIOgZZQhOzBidV2gZYZ1\XJiNEigbJJ{KGK7IF3UV{Bie3OjeR?= MVuxPFI2QDR2MB?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
FADD / PARP / Cleaved PARP ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of FADD, PARP, and cleaved PARP were then measured by Western blot analysis.

Caspase-3 / Cleaved Caspase-3 / Caspase-8 / Cleaved Caspase-8 ; 

PubMed: 30992674     


LN229 cells were exposed to luteolin (0, 10, 20, and 30 µM) for 24 hours; the levels of Caspase-8, cleaved Caspase-8, Caspase-3, and cleaved Caspase-3 were measured by Western blot analysis. 

ERK / p-ERK / JNK / p-JNK / p38 / p-p38 / Bax / Bcl-2 ; 

PubMed: 30992674     


LN229 cells were treated with luteolin (0, 10, 20, and 30 µM) for 24 hours, and Western blot analysis was performed to measure the expression levels of ERK, P-ERK, JNK, P-JNK, p38, P-p38, Bax, and Bcl-2. 

p-VEGFR2 / p-mTOR / pS6K1 / p70S6K1 / pAKT / AKT / MMP-2 / MMP-9 ; 

PubMed: 23300633     


Luteolin suppressed the activation of VEGFR2 and their downstream signaling pathway triggered by VEGF in HUVECs. Proteins from different treatments was tested by Western blotting and probed with specific antibodies. Experiments were repeated for three times. 

p21 / Survivin / Cyclin D1; 

PubMed: 30930986     


Total protein was isolated from control and luteolin-treated MDA-MB-231 cells, and subjected to immunoblotting of p21, Survivin and cyclin D1 proteins. Membranes were stripped and re-probed with anti-GAPDH antibody to ensure equal protein loading. p21 was upregulated, whereas Survivin and cyclin D1 were downregulated in MDA-MB-231 cell in a dose-dependent manner. *P<0.05 vs. the control group (0 µM luteolin); #P<0.05 vs. the 10 µM luteolin group.

DNMT1 / DMNT3A / DNMT3B ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against DNMT1, DNMT3A, DNMT3B.

TET1 / TET2 / TET3 ; 

PubMed: 30988303     


Cells were treated with various concentrations (0–60 μM) of luteolin for 48 h Western blot analysis was performed using antibodies against TET1, TET2, and TET3.

30992674 23300633 30930986 30988303
Immunofluorescence
5-hmC ; 

PubMed: 30988303     


Formation of 5-hmC by TETs was assessed using confocal imaging.

30988303
Growth inhibition assay
Cell viability; 

PubMed: 30992674     


(B) LN229 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (C) U251 cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay. (D) HEB cells were incubated with different concentrations of luteolin for 24 and 48 hours, and then the cell viability was measured by CCK-8 assay.

30992674
In vivo LD50: Mice >2500mg/kg (i.g.) [3]

Protocol

Solubility (25°C)

In vitro DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL warmed (10.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 286.24
Formula

C15H10O6

CAS No. 491-70-3
Storage powder
in solvent
Synonyms Luteoline, Luteolol, Digitoflavone
Smiles C1=CC(=C(C=C1C2=CC(=O)C3=C(C=C(C=C3O2)O)O)O)O

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Frequently Asked Questions

  • Question 1:

    Would you please suggest a suitable vehicle to dissolve Luteolin for in vivo i.p. use?

  • Answer:

    Formula: 5% DMSO+40% PEG 300+5% Tween80+ddH2O, working Solution concentration: up to 7.5mg/ml, stable for 30min.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID