Furosemide (NSC 269420)

For research use only.

Catalog No.S1603

4 publications

Furosemide (NSC 269420) Chemical Structure

CAS No. 54-31-9

Furosemide (NSC 269420) is a potent NKCC2 (Na-K-2Cl symporter) inhibitor, used in the treatment of congestive heart failure and edema.

Selleck's Furosemide (NSC 269420) has been cited by 4 publications

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Biological Activity

Description Furosemide (NSC 269420) is a potent NKCC2 (Na-K-2Cl symporter) inhibitor, used in the treatment of congestive heart failure and edema.
Targets
NKCC2 [4]
In vitro

Furosemide reversibly alters the responses to tones and clicks of the chinchilla basilar membrane in hair cells, causing response-magnitude reductions that are largest (up to 61 dB, averaging 25-30 dB) at low stimulus intensities at the characteristic frequency (CF) and small or nonexistent at high intensities and at frequencies far removed from CF. Furosemide also induces response-phase lags that are largest at low stimulus intensities (averaging 77 degrees) and are confined to frequencies close to CF. [1] Furosemide concentration- and time-dependently increases the formation of nitric oxide andprostacyclin. Furosemide leads to an enhanced release of kinins into the supernatant of the cells. [2] Furosemide reversibly suppresses low Ca2+-induced epileptiform activity in hippocampus proper and blocks or significantly reduces different types of epileptiform discharges in the low Mg2+ model and the 4-aminopyridine model. [3] Furosemide significantly inhibits cell growth in MKN45 cells, but not in MKN28 cells. Furosemide diminishes cell growth by delaying the G(1)-S phase progression in poorly differentiated gastric adenocarcinoma cells, which show high expression and activity of NKCC, but not in moderately differentiated gastric adenocarcinoma cells with low expression and NKCC activity. [4]

In vivo Furosemide (0.25 to 2 mg/kg), administered 4 hours before exercise, reduces right atrial pressure (RAP) and pulmonary arterial pressure (PAP) during exercise in dose-dependent manner in horse. [5]

Protocol

Solubility (25°C)

In vitro DMSO 66 mg/mL (199.55 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 330.74
Formula

C12H11ClN2O5S

CAS No. 54-31-9
Storage powder
in solvent
Synonyms N/A
Smiles C1=COC(=C1)CNC2=CC(=C(C=C2C(=O)O)S(=O)(=O)N)Cl

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04972617 Not yet recruiting Diagnostic Test: albumin function analysis (ABIC) Acute Renal Failure|Critical Illness University of Rostock September 2021 --
NCT04331132 Not yet recruiting Drug: Tolvaptan 15 MG Acute Heart Failure Gia Dinh People Hospital|Otsuka Pharmaceutical Vietnam August 1 2021 Not Applicable
NCT04846816 Recruiting Combination Product: SQIN-01 Heart Failure SQ Innovation Inc.|University of Glasgow|NHS Greater Glasgow and Clyde May 6 2021 Phase 1
NCT04622709 Recruiting Drug: Furosemide (loop diuretic) Tablets Chronic Kidney Disease Requiring Chronic Dialysis University of North Carolina Chapel Hill|National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) October 7 2020 Phase 2
NCT04556864 Not yet recruiting -- Covid19|Hemodynamic Instability|ARDS Human Juan Victor Lorente|Edwards Lifesciences|Andalusian Network for Design and Translation of Advanced Therapies October 1 2020 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID