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5 Lipoxygenase/5-LO Rabbit mAb

Catalog No.: F1022

Application: Reactivity: Rat, Human

Usage Information

Dilution
WB1:1000 - 1:2000 IHC1:100 IF1:100

Application
WB, IHC, IF, FCM

Reactivity
Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
78 kDa

Datasheet & SDS

Biological Description

Specificity
5 Lipoxygenase/5-LO Rabbit mAb detects endogenous levels of total 5 Lipoxygenase/5-LO protein.

Clone
C10C20

Synonym(s)
LOG5, ALOX5, Polyunsaturated fatty acid 5-lipoxygenase, Arachidonate 5-lipoxygenase, 5-LO, 5-lipoxygenase

Background
5-Lipoxygenase (5-LO) is a non-heme iron-containing monomeric enzyme of the lipoxygenase family that catalyzes the initial steps of leukotriene biosynthesis by oxygenating arachidonic acid into 5-HPETE and subsequently dehydrating it to leukotriene A4 (LTA4). 5-LO is composed of two main domains: an N-terminal β-sandwich C2-like domain responsible for calcium and membrane binding, and a C-terminal catalytic domain housing a non-heme iron coordinated by a 2-His-1-carboxylate facial triad essential for its dioxygenase function. The enzyme is activated by calcium ions, membrane association, and ATP binding, which stabilizes its conformation and enhances enzymatic activity. 5-LO interacts with accessory proteins like coactosin-like protein (CLP) to promote catalysis. The enzyme plays a fundamental role in producing leukotrienes, which are potent lipid mediators regulating inflammation, immune cell chemotaxis, vasoconstriction, and bronchoconstriction. 5-LO expression is primarily in leukocytes, including neutrophils, eosinophils, macrophages, and mast cells. Its activity is tightly regulated transcriptionally and post-translationally through phosphorylation, calcium signaling, and intracellular localization to maintain leukotriene production in response to stimuli. Additionally, 5-LO translocates to the nucleus, where it associates with euchromatin and influences gene expression and microRNA processing independent of its enzymatic role. Dysregulated 5-LO activity is implicated in diseases such as asthma, atherosclerosis, and cancer.

Background

5-Lipoxygenase (5-LO) is a non-heme iron-containing monomeric enzyme of the lipoxygenase family that catalyzes the initial steps of leukotriene biosynthesis by oxygenating arachidonic acid into 5-HPETE and subsequently dehydrating it to leukotriene A4 (LTA4). 5-LO is composed of two main domains: an N-terminal β-sandwich C2-like domain responsible for calcium and membrane binding, and a C-terminal catalytic domain housing a non-heme iron coordinated by a 2-His-1-carboxylate facial triad essential for its dioxygenase function. The enzyme is activated by calcium ions, membrane association, and ATP binding, which stabilizes its conformation and enhances enzymatic activity. 5-LO interacts with accessory proteins like coactosin-like protein (CLP) to promote catalysis. The enzyme plays a fundamental role in producing leukotrienes, which are potent lipid mediators regulating inflammation, immune cell chemotaxis, vasoconstriction, and bronchoconstriction. 5-LO expression is primarily in leukocytes, including neutrophils, eosinophils, macrophages, and mast cells. Its activity is tightly regulated transcriptionally and post-translationally through phosphorylation, calcium signaling, and intracellular localization to maintain leukotriene production in response to stimuli. Additionally, 5-LO translocates to the nucleus, where it associates with euchromatin and influences gene expression and microRNA processing independent of its enzymatic role. Dysregulated 5-LO activity is implicated in diseases such as asthma, atherosclerosis, and cancer.

References
https://pubmed.ncbi.nlm.nih.gov/10591081/ https://pubmed.ncbi.nlm.nih.gov/18987389/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%