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Anti-SOD2/MnSOD Rabbit Antibody [M14N10]

Catalog No.: F3964

Application: Reactivity: Mouse, Rat, Human

Usage Information

Dilution
WB1:1000 - 1:2000 IHC1:100 - 1:250

Application
WB, IHC

Reactivity
Mouse, Rat, Human

Source
Rabbit

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW Observed MW
25 kDa 25 kDa
^*Why do the predicted and actual molecular weights differ? The following reasons may explain differences between the predicted and actual protein molecular weight.

Positive Control	Human liver tissue; Human heart tissue; Rat heart tissue; Rat brain lysate; SH-SY5Y; A549; HEK-293T; HAP1
Negative Control

Datasheet & SDS

Biological Description

Specificity
SOD2/MnSOD Rabbit mAb detects endogenous levels of total SOD2/MnSOD protein.

Clone
M14N10

Synonym(s)
SOD2

Background
Manganese superoxide dismutase (MnSOD or SOD2) is a key mitochondrial enzyme that defends cells against oxidative stress by converting harmful superoxide radicals (O₂⁻) into oxygen (O₂) and hydrogen peroxide (H₂O₂). Functioning as a homotetramer, each MnSOD subunit houses an active site with a manganese ion coordinated by conserved residues (His26, His74, His163, and Asp159). This metal center cycles between Mn³⁺ and Mn²⁺ states, enabling catalysis through coordinated proton and electron transfers involving key amino acids such as His30, Tyr34, and Gln143. The enzyme operates via two catalytic pathways: a fast outer-sphere electron transfer and a slower inner-sphere mechanism involving peroxy adducts. MnSOD plays a central role in limiting mitochondrial reactive oxygen species (ROS), byproducts of the electron transport chain, thus protecting mitochondrial proteins, lipids, and DNA. Its activity is finely regulated at transcriptional and post-translational levels, and changes in metal cofactor specificity (switching from manganese to iron) can impair its function. MnSOD participates in redox signaling, with H₂O₂ acting as a signaling molecule in processes like apoptosis, cell proliferation, and inflammation. Deficiency or dysfunction of MnSOD is associated with diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, due to the resulting oxidative damage.

Background

Manganese superoxide dismutase (MnSOD or SOD2) is a key mitochondrial enzyme that defends cells against oxidative stress by converting harmful superoxide radicals (O₂⁻) into oxygen (O₂) and hydrogen peroxide (H₂O₂). Functioning as a homotetramer, each MnSOD subunit houses an active site with a manganese ion coordinated by conserved residues (His26, His74, His163, and Asp159). This metal center cycles between Mn³⁺ and Mn²⁺ states, enabling catalysis through coordinated proton and electron transfers involving key amino acids such as His30, Tyr34, and Gln143. The enzyme operates via two catalytic pathways: a fast outer-sphere electron transfer and a slower inner-sphere mechanism involving peroxy adducts. MnSOD plays a central role in limiting mitochondrial reactive oxygen species (ROS), byproducts of the electron transport chain, thus protecting mitochondrial proteins, lipids, and DNA. Its activity is finely regulated at transcriptional and post-translational levels, and changes in metal cofactor specificity (switching from manganese to iron) can impair its function. MnSOD participates in redox signaling, with H₂O₂ acting as a signaling molecule in processes like apoptosis, cell proliferation, and inflammation. Deficiency or dysfunction of MnSOD is associated with diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions, due to the resulting oxidative damage.

References
https://pubmed.ncbi.nlm.nih.gov/21434856/ https://pubmed.ncbi.nlm.nih.gov/35662317/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%