Anti-RANK Mouse Antibody [E4N12]

Catalog No.: F2425

Application: Reactivity: Human, Mouse

Usage Information

Dilution
IP1:500-1:1000 IHC1:200

Application
IP, IHC

Reactivity
Human, Mouse

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Positive Control	Human skin; Human t cell lymphoma; RAW cell
Negative Control

Datasheet & SDS

Biological Description

Specificity
RANK Mouse mAb detects endogenous levels of total RANK protein.

Clone
E4N12

Synonym(s)
CD265, RANK, TNFRSF11A, Tumor necrosis factor receptor superfamily member 11A, Osteoclast differentiation factor receptor, Receptor activator of NF-KB, ODFR

Background
RANK (Receptor Activator of Nuclear Factor κB) is a type I transmembrane glycoprotein of 616 amino acids, comprising a 28-amino-acid signal peptide, a 184-amino-acid extracellular domain, a 21-amino-acid transmembrane region, and a 383-amino-acid intracellular domain containing three functional cytoplasmic motifs—PFQEP(369–373), PVQEET(559–564), and PVQEQG(604–609)—that mediate interactions with adaptor proteins such as TRAF6 to activate NF-κB, MAPK (JNK, ERK, p38), and Akt/PKB pathways. Encoded by the TNFRSF11A gene, RANK is expressed in osteoclast precursors, dendritic cells, monocytes, macrophages, activated T cells, natural killer cells, thymic medullary epithelial cells, mammary epithelial cells, and certain epithelial stem cell populations. Functionally, RANK regulates osteoclast differentiation, activation, and survival in bone remodeling; supports lymph node organogenesis, thymic epithelial development, and immune tolerance; maintains epithelial stem cell homeostasis; mediates progesterone-driven mammary epithelial proliferation; and, when dysregulated, contributes to tumorigenesis, metastasis, and cancer stem cell expansion.

Background

RANK (Receptor Activator of Nuclear Factor κB) is a type I transmembrane glycoprotein of 616 amino acids, comprising a 28-amino-acid signal peptide, a 184-amino-acid extracellular domain, a 21-amino-acid transmembrane region, and a 383-amino-acid intracellular domain containing three functional cytoplasmic motifs—PFQEP(369–373), PVQEET(559–564), and PVQEQG(604–609)—that mediate interactions with adaptor proteins such as TRAF6 to activate NF-κB, MAPK (JNK, ERK, p38), and Akt/PKB pathways. Encoded by the TNFRSF11A gene, RANK is expressed in osteoclast precursors, dendritic cells, monocytes, macrophages, activated T cells, natural killer cells, thymic medullary epithelial cells, mammary epithelial cells, and certain epithelial stem cell populations. Functionally, RANK regulates osteoclast differentiation, activation, and survival in bone remodeling; supports lymph node organogenesis, thymic epithelial development, and immune tolerance; maintains epithelial stem cell homeostasis; mediates progesterone-driven mammary epithelial proliferation; and, when dysregulated, contributes to tumorigenesis, metastasis, and cancer stem cell expansion.

References
https://pubmed.ncbi.nlm.nih.gov/33552088/ https://pubmed.ncbi.nlm.nih.gov/26749530/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%