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Anti-p44/42 MAPK (Erk1/2) Mouse Antibody [E11P7]

Catalog No.: F3509

Application: Reactivity: Human, Mouse, Rat, Monkey, Mink, Zebrafish, Bovine, Pig

Usage Information

Dilution
WB1:2000 IHC1:250 - 1:1000 IF1:100

Application
WB, IHC, IF

Reactivity
Human, Mouse, Rat, Monkey, Mink, Zebrafish, Bovine, Pig

Source
Mouse

Storage Buffer
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage (from the date of receipt)
-20°C (avoid freeze-thaw cycles), 2 years

Predicted MW
42 kDa, 44 kDa

Positive Control	Human breast carcinoma; Human colon carcinoma; NIH/3T3 (U0126, 10 μM, 2 h or PDGF, 100 ng/ml, 20 min); NIH/3T3; Jurkat (TPA, 200 nM, 20 mins)
Negative Control

Datasheet & SDS

Biological Description

Specificity
p44/42 MAP Kinase Mouse mAb detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some systems this antibody may recognize p42/Erk2 more readily than p44/Erk1. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase.

Clone
E11P7

Synonym(s)
Mitogen-activated protein kinase 3, MAP kinase 3; MAPK 3, ERT2, Extracellular signal-regulated kinase 1 (ERK-1), Insulin-stimulated MAP2 kinase, MAP kinase isoform p44 (p44-MAPK), Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 1, MAP kinase 1; MAPK 1, ERT1, Extracellular signal-regulated kina

Background
Mitogen-activated protein kinases p42 and p44 (also known as Erk2 and Erk1) are critical mediators that relay signals from the cell surface to the nucleus. The p42/p44 MAPK pathway transmits inputs from receptor tyrosine kinases, certain G protein–coupled receptors, cytokine receptors, and integrins via a Ras- Raf-MAP kinase kinase (MEK) -p42/p44 MAPK activation cascade. Among all components of this cascade, only the p44 and p42 MAPK isoforms (Erk1 and Erk2) are capable of accumulating in the nucleus following mitogenic stimulation, where they phosphorylate numerous transcription factors. This signaling pathway can be triggered by a wide array of extracellular cues, including mitogens, growth factors, and cytokines. Activation follows a conserved three-tier kinase cascade: a MAP kinase kinase kinase (MAPKKK or MAP3K) activates a MAP kinase kinase (MAPKK or MAP2K), which in turn activates a MAP kinase (MAPK). Multiple MAP3Ks can initiate this pathway, notably the Raf family kinases as well as Mos and Tpl2/COT. MEK1 and MEK2 serve as the principal MAPKKs, phosphorylating p44 and p42 on activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively, to drive their activation.

Background

Mitogen-activated protein kinases p42 and p44 (also known as Erk2 and Erk1) are critical mediators that relay signals from the cell surface to the nucleus. The p42/p44 MAPK pathway transmits inputs from receptor tyrosine kinases, certain G protein–coupled receptors, cytokine receptors, and integrins via a Ras- Raf-MAP kinase kinase (MEK) -p42/p44 MAPK activation cascade. Among all components of this cascade, only the p44 and p42 MAPK isoforms (Erk1 and Erk2) are capable of accumulating in the nucleus following mitogenic stimulation, where they phosphorylate numerous transcription factors. This signaling pathway can be triggered by a wide array of extracellular cues, including mitogens, growth factors, and cytokines. Activation follows a conserved three-tier kinase cascade: a MAP kinase kinase kinase (MAPKKK or MAP3K) activates a MAP kinase kinase (MAPKK or MAP2K), which in turn activates a MAP kinase (MAPK). Multiple MAP3Ks can initiate this pathway, notably the Raf family kinases as well as Mos and Tpl2/COT. MEK1 and MEK2 serve as the principal MAPKKs, phosphorylating p44 and p42 on activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively, to drive their activation.

References
https://pubmed.ncbi.nlm.nih.gov/9700154/ https://pubmed.ncbi.nlm.nih.gov/16170216/

Reference Table for Selecting PVDF Membrane Pore Size Specifications

Protein Size (kDa)	PVDF Transfer Membrane Pore Size (μm)
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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Protein Size (kDa)	Separating Gel Percentage
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%